Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Evaluation of Antinociceptive and Antioxidant Properties of the Ethanolic Extract of Sonneratia Caseolaris Leaves

Md. Sariful I. Howlader
Department of Pharmacy, World University of Bangladesh, Dhaka-1205, Bangladesh.

Shubhra K. Dey
Pharmacy Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh.

Arpona Hira
Pharmacy Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh.

Arif Ahmed
Pharmacy Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh.

Abstract/ Executive Summary:

The ethanolic extract of leaves of Sonneratia caseolaris exhibited statistically significant (p>0.01 & p>0.001) writhing inhibition in acetic acid induced writhing model in white albino mice (Swiss-webstar strain). The crude extract produced 24.37% inhibition of writhing at the dose of 250 mg/kg body weight & 43.15 % inhibition of writhing at the dose of 500 mg/kg body weight while the standard drug diclofenac inhibition was found to 65.48 % at a dose of 25 mg/kg body weight. The antioxidant property of ethanolic extract of S. caseolaris was assessed by DPPH (1, 1-diphenyl-2-picryl hydrazyl) free radical scavenging activity. In DPPH scavenging assay the IC 50 value was found to be (68 µg/ml) which was comparable to the standard ascorbic acid (13 µg/ml). Phytochemical nature (group determination of plant constituent) and selected phytochemical analysis of the ethanolic extract of the leaves of S. caseolaris indicated the presence of flavonoid, reducing sugars, tannins & saponins types of compounds. The obtained results provide a support for the use of this plant in traditional medicine and its further investigation. The present study tends to suggest the antinociceptive and antioxidant activities of the crude ethanolic extract of the leaves of S. caseolaris and justify its use in folkloric remedies.

Keywords: Sonneratia caseolaris, Antinociceptive, Antioxidant

Location: Khulna, Bangladesh

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Mangrove

Objectives:

To investigate the antinociceptive and antioxidant activities that confirm its use as pain killer and as an antioxidant.

Methodology:

S. caseolaris was collected from Karamjal, Sundarban Khulna, Bangladesh. A specimen copy was deposited to Bangladesh National Herbarium for identification & the accession number DACB-29787. The leaves of S. caseolaris were freed from any of the foreign materials. Then the leaves were air-dried under shed temperature followed by drying in an electric oven at 40º C. The dried plant materials were then ground into powder. About 350 g of powdered material was taken in a clean, flat-bottomed glass container and soaked in 900 ml of 95% ethanol. The container with its contents was sealed and kept for a period of 10 days accompanying occasional shaking and stirring. The whole mixture then underwent a coarse filtration by a piece of clean, white cotton material. Then it was filtered through whatman filter paper. Which was concentrated with rotary evaporator at bath temperature not exceeding 40? to have gummy concentrate of extract (yield approx. 2.77%). The freshly prepared crude extract was qualitatively tested for the presence of chemical constituents, by using the following reagents and chemicals, for example, alkaloids were identified by the dragendorff’s reagent, flavonoids with the use of Mg and HCl, tannins with ferric chloride and potassium dichromate solutions, and steroids with Libermann-Burchard reagent. Reducing sugars with benedict’s reagent Young Swiss-albino mice male sex,3-4 weeks of age, weighing 20 -25g, were used for in vivo pharmacological screening. Mice were purchased from the Animal Research Branch of the International Centre for Diarrhoeal Disease and Research, Bangladesh (ICDDR,B). They were housed in standard environmental conditions at animal house of Khulna University animal lab and fed with rodent diet and water ad libitum. All experimental protocols were in compliance with BCSIR Ethics Committee on Research in Animals as well as internationally accepted principles for laboratory animal use and care. The standard drug Diclofenac Na was used for this study and purchased from Square Pharmaceuticals Ltd, Bangladesh. The antinociceptive activity of the crude ethanolic extract of S. caseolaris was studied using acetic acid induced writhing model in mice. The animals were divided into control, positive control and test groups with five mice in each group. The animals of test groups received test substance at the dose of 250 and 500 mg/kg body weight. Positive control group was administered with Diclofenac Na (standard drug) at the dose of 25 mg/kg body weight and vehicle control group was treated with 1% Tween 80 in water at the dose of 10 ml/kg body weight. Test samples, standard drug and control vehicle were administered orally 30 min before intraperitoneal administration of 0.7% acetic acid. After an interval of 15 min, the mice were observed writhing (constriction of abdomen, turning of trunk and extension of hind legs) for 5 min. Quantitative assay was performed on the basis of the modified method. Stock solutions (10 mg/ml) of the plant extracts were prepared in ethanol from which serial dilutions were carried out to obtain concentrations of 1, 5, 10, 25, 50, 100, 200 and 500 mg/ml. Diluted solutions (2 ml) were added to 2 ml of a 0.004% ethanol solution of DPPH, mixed and allowed to stand for 30 min for reaction to occur. The absorbance was determined at 517 nm and from these values corresponding percentage of inhibitions were calculated. Then % inhibitions were plotted against concentration and from the graph IC 50 was calculated. The experiment was performed in duplicate and average absorption was noted for each concentration. Ascorbic acid was used as positive control. (DPPH) free radical scavenging activity was determined by the method. Plant extract (0.1 mL) was added to 3 mL of a 0.004% MeOH solution of DPPH. For analgesic determination, data were presented as mean ± Standard deviation (S.D). Statistical analysis for animal experiment was carried out using one-way ANOVA followed by Dunnet’s multiple comparisons. The results obtained were compared with the control group p values < 0.01 & < 0.001 were considered to be statistically significant (p indicates probability).

Source of Information: Der Pharmacia Sinica, 2012, 3 (5):536-541, ISSN: 0976-8688

Article Link (Online Journal): www.pelagiaresearchlibrary.com

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It can be revealed that the crude ethanolic extract of S. caseolaris leaves possess significant antinociceptive as well as antioxidant activities. The potential extract of S. caseolaris as antinociceptive and antioxidant agents may be due to the presence of phytoconstituents like tannins, phenolics etc and might be responsible for its activity and justify its use as a traditional folk remedy.

Knowledge Management: Journal