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Yield Response of Black Gram to Inoculation by Different Rhizobium Strains Using Various Types of Adhesives

M.A. Saleh
Department of Genetic Engineering and Biotechnology, University of Rajshahi, Bangladesh.

S. Zaman
Department of Genetic Engineering and Biotechnology, University of Rajshahi, Bangladesh.

G. Kabir
Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh.

Abstract/ Executive Summary:

To evaluate the effect of different Rhizobium strains on yield of Black Gram, a field experiment was conducted in the field of Rajshahi University, Bangladesh. Three Rhizobium strains (RLc 107, RCa 220 and RVm 307), four different types of adhesives (Sucrose, Peptone, Molasses and Glycerol) and two Black Gram varieties (BARI MASH-1 and BINA MASH-1) were used as an experimental material. Data were recorded on number of pods per plant, number of seeds per plant, 100-seed weight and seed yield per plant. The yield of Black gram was significantly affected by Rhizobium inoculation and the better result for all the characters were obtained in BINA MASH-1 when seeds were inoculated with RVm 307 using the adhesive Peptone.

Keywords: Adhesives, Black gram, Rhizobium Strains , Yield

Location: University of Rajshahi, Bangladesh.

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Black gram

Objectives:

To investigate the effect of three Rhizobium strains on yield of Black Gram using four different types of adhesives.

Methodology:

To evaluate the response of Black Gram to Rhizobium inoculation, a field experiment was conducted at the University of Rajshahi, Bangladesh. For this study, two Black Gram varieties i.e., BARI MASH 1 and BINA MASH 1; three Rhizobium strains RVm 307 (isolated from Black Gram), RCa 220 (isolated from Chick Pea) and Rlc107 (isolated from Lentil); four different types of adhesives namely Sucrose, Peptone, Molasses and Glycerol were used as an experimental materials. The seeds of Black Gram varieties were disinfected with 0.2% HgCl₂ (3-4 min) followed by 6-7 washings with sterile water. The disinfected seeds were then suspended in 50 mL thick suspension (10¹² cells mL^-1) of Rhizobium in presence of 1% sucrose, peptone, molasses and glycerol separately for 30 min. These seeds were air dried and sown in the field with three replications. Data on number of pods per plant, number of seeds per plant, 100-seed weight and seed yield per plant was taken and analyzed statistically.

Source of Information: Asian Journal of Biological Sciences Volume 6, Number 3, 181-186, 2013

Article Link (Online Journal): DOI: 10.3923/ajbs.2013.181.186, URL: http://scialert.net/abstract/?doi=ajbs.2013.181.186

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Among the three different Rhizobium strains, RVm 307 (isolated from Black Gram) was found to be the most effective strain to enhance the yield of Black Gram.

Knowledge Management: Journal