Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Establishment of in Vitro Regeneration Protocol for Adhatoda Vasica Nees.

S Khan
Plant Tissue Culture Section, Biological Research Division, BCSIR Laboratories, Dhaka, Bangladesh Council ofScientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

S Akter
Plant Tissue Culture Section, Biological Research Division, BCSIR Laboratories, Dhaka, Bangladesh Council ofScientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

A Habib
Plant Tissue Culture Section, Biological Research Division, BCSIR Laboratories, Dhaka, Bangladesh Council ofScientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

T A Banu
Plant Tissue Culture Section, Biological Research Division, BCSIR Laboratories, Dhaka, Bangladesh Council ofScientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

M Islam
Plant Tissue Culture Section, Biological Research Division, BCSIR Laboratories, Dhaka, Bangladesh Council ofScientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

NF Khan
Plant Tissue Culture Section, Biological Research Division, BCSIR Laboratories, Dhaka, Bangladesh Council ofScientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

S Afrin
Plant Tissue Culture Section, Biological Research Division, BCSIR Laboratories, Dhaka, Bangladesh Council ofScientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

A Ferdousi
Plant Tissue Culture Section, Biological Research Division, BCSIR Laboratories, Dhaka, Bangladesh Council ofScientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

S Islam
Plant Tissue Culture Section, Biological Research Division, BCSIR Laboratories, Dhaka, Bangladesh Council ofScientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

Abstract/ Executive Summary:

An in vitro regeneration protocol of Adhatoda vasica was developed using excised nodal segments and juvenile leaves for multiple shoots regeneration directly or through callus induction. Explants were cultured on MS medium with different concentrations of IAA, NAA, BAP, GA₃ and Kn singly or in combinations. MS medium supplemented with BAP (10.0 mg/l) was found best for multiple shoot formation, in which 93.33% explants produced multiple shoots. After two months, maximum number of multiple shoots were 10.6 ± 1.82, highest length of plantlets was 5.2 ± 2.20 cm. 100% calli formation were observed on MS medium supplemented with IAA (0.05 mg/l) + NAA (0.05 mg/l) + BAP (1.0 mg/l). Callus initiation started after 14 days and gave light green colored callus. Best callus mediated shoot regeneration was found on MS+10.0 mg/l BAP medium. Root induction of in vitro raised shoots was best on ½ MS + IBA (1.0 mg/l). Well rooted plantlets were transferred to plastic pots containing garden soil and compost in a ratio of 2:1 for hardening. The ultimate survival rate under natural condition was about 80%.

Keywords: Adhatoda vasica,Nodal segment, Leaf explants, Multiple shoot, Callus induction

Location: Plant Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka.

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

To develop a suitable in vitro regeneration protocol of Adhatoda vasica Nees.

Methodology:

The present experiment was conducted in Plant Tissue Culture Section of the Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka. Fresh young leaves and nodal segments of Adhatoda vasica were collected from the plants grown in the BCSIR research field. The fresh juvenile leave, nodal segments and split nodal halves were then used for callus induction and direct regeneration respectively. Leaves and nodal segments of Adhatoda vasica were taken in a 500 ml conical flask for surface sterilization. These explants were kept under running tap water for 30 minutes, then washed with mild detergent and washed with tap water. After this, the explants were taken into laminar airflow cabinet, added 70% alcohol for 1 minute then rinsed off with sterile distilled water. Finally added 0.1% HgCl₂ (w/v) for 5 minutes for nodal explants and 3 minutes for leaf explants with continuous stirring followed by washing 3 times with sterile distilled water. Surface sterilized leaves were cut into small pieces and 3-4 excised pieces were taken into a conical flask containing agar solidified MS medium supplemented with various concentrations of BAP, NAA, Kn, IAA and GA₃ alone or in combinations for callus induction. All media contained 3% sucrose and 7.0 gm/l agar with pH 5.8 adjusted before autoclaving. Green callus was excised and transferred to fresh MS medium containing similar compositions of plant growth regulators for organogenesis. Nodal explants were cut into one single node and inoculated vertically onto test tubes containing shoot regeneration media. In each test tube a single cutting was placed. All in vitro grown cultures were maintained under illumination on a 12h photo period at 25 ± 2 ºC. Regenerated shoots were sub- cultured every four weeks on the freshly prepared same medium. When the regenerated shoots were 4-5 cm in length, they were transferred to freshly prepare medium containing half strength of MS and MS medium with different concentrations of IBA for root induction. Rooted plantlets were transferred to small plastic pots containing sterilized soil and covered with polythene bags to maintain high humidity. After 50 days the acclimatized plants were transferred to larger pots containing garden soil and compost in ratio of 2 : 1 and moist them adequately for proper hardening.

Source of Information: Bangladesh J. Sci. Ind. Res. 51(1), 75-80, 2016, ISSN 0304-9809

Article Link (Online Journal): DOI: http://dx.doi.org/10.3329/bjsir.v51i1.27077

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In the study. MS media in combination with different hormonal supplements namely, IAA, NAA, IBA, BAP, GA₃ and Kn were used singly or in combinations to observe their effect on multiple shoot development form the shoot tip and nodal segment. Among the media components used, MS medium supplemented with BAP (10.0 mg/l) showed best response for multiple shoot formation.

Knowledge Management: Journal