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Effect of Different Storage Containers Used for Storing Seeds and Management Practices on Seed Quality and Seed Health in Cvl-1 Variety

S.M.A. Haque
Department of Plant Pathology, Pest Management Division, Bangladesh Jute Research Institute, Dhaka.

I. Hossain
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh.

M.A. Rahman
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh.

Abstract/ Executive Summary:

The experiments were conducted in the laboratory of Department of Plant Pathology, Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka; Seed Pathology Center, Bangladesh Agricultural University (BAU), Mymensingh, Oil Seed Division, Bangladesh Agricultural Research Institute (BARI). The experiments were conducted during the period 15 January 2010 to 5 March 2012. Nine different types of containers viz. tin pot, plastic pot, poly bag, gunny bag, gunny bag lined with polythene, earthen pot, cloth bag, brown paper and IRRI poly bag, two level of moisture contents viz. farmers’ condition (16%) and recommended moisture condition (9.5%) by Bangladesh Gazette in 2010 and seed treatment with Provax-200 and control (untreated) were used for the present study. Seeds were stored for 12 months and examined after 4, 8 and 12 months of storage. Among the nine containers, tin pot was found better in respect of moisture content, germination, mean germination time, vigour index and 1000 seed weight. Health condition of seeds was also superior compared to other containers. The poorest performance was observed in earthen pot regarding moisture content, germination, mean germination time, vigour index, 1000 seed weight and seed borne infection. The findings of the present study also reveal that recommended moisture content (9.5% moisture content in seed) was better than moisture content at farmers’ condition. Provax-200 treated seed also resulted better performance than non treated (control condition) seed. So, quality of jute seeds can be maintained by storage in tin pot with provax-200 treated and recommendation moisture content (9.5%).

Keywords: Effect, Storage container, Management, Storage condition, Seed quality and health

Location: Laboratory of Plant Pathology Department, Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka

Start Date: 15-01-2010

End Date: 05-03-2012

Program Area: Postharvest and Agro-processing

Commodity: Jute

Objectives:

To develop an eco-friendly model of safe storing methods for quality jute seeds.

Methodology:

The experiments were conducted in the laboratory of Plant Pathology Department, Bangladesh Jute Research Institute (BJRI), Manik Mia Avenue, Dhaka; Seed Pathology Center, Bangladesh Agricultural University (BAU), Mymensingh, Oil Seed Division, Bangladesh Agricultural Research Institute (BARI). The experiments were conducted during the period 15 January 2010 to 5 March 2012. Varieties of seed: Seed of CVL-1 was used in this study and 110 kg CVL-1 seeds collected from one farmer in Savar, Dhaka. Containers for experiment: For this experiment, nine different types of storage containers were used, viz. T₁= Tin pot, T₂= Plastic pot, T₃ = Poly bag having 25 μm thickness, T₄ = Gunny bag, T₅ = Gunny bag lined with polythene, T₆= Earthen pot, T₇ = Cloth bag, T₈= Brown paper and T₉ = IRRI Poly bag (Super Grain bag II Z) having 78 μm thickness. Seed samples were obtained from the seed lots of each variety. Seed samples were mixed thoroughly and were divided equally into two parts. Half of seeds of both samples were mixed thoroughly with Provax-200 WP (0.4% of seed weight) and then kept into nine different containers and rest of unmixed seeds of samples treated as control kept in other nine different containers. All the containers had three replications and were labeled properly and preserved in room temperature till the samples were used for further study. Before storing, quality and health status of collected seed samples were tested. The quality and health test of stored seeds were done for three times viz. (1) After 4 months of storing, (2) After 8 months of storing and (3) After 12 months of storing. Jute seed moisture was determined by constant temperature oven dry method. The amounts of working samples depended on the diameter of the container which was used for determination of the moisture of the seed samples. The diameter of the container was less than 8 cm and the amount of seed samples were 4-5 g. The working sample was evenly distributed over the surface of the container. The weight of the container and its lid were recorded before and after filling the container with the seed samples. The container was placed rapidly on the tops of its lid and placed in an oven maintaining the temperature of 103±2°C and dried for 17±1 h. At the end of the prescribed period, the containers were covered with lids and placed in the desiccators to make it cool for 30-40 min. After cooling, weight of the container with its lid and contents were recorded. The relative humidity of the ambient air in the laboratory at the time of moisture determination was less than 70%. Two hundred seeds were taken randomly from the well mixed seed sample. The working samples were divided into eight replications and thus one replication contained 25 seeds. The seeds were germinated on top of three layers of Whatmann No. 1 filter paper. The filter papers were soaked in water and placed at the bottom of 9 cm diameter plastic petridish and thereafter 25 seeds were placed on the top of filter paper. Evaporation of water was minimized by tightly fitting the lids of the petridishes. The petridishes were placed inside the incubator maintaining the temperature at 30°C for five days. Seeds producing both plumule and radical after incubation were counted as germinating seeds. Seed health analysis was conducted by blotter method following the International Rules for Seed Health Testing. In this method, 9 cm diameter plastic petridish and locally packed Whatman No. 1 filter paper was used. Two hundred seeds from each sample were taken randomly and placed on the moist filter paper in eight replicate petridish. The petridishes with seeds were incubated at 22+2°C, 12/12 h alternating cycles of near ultraviolet (NUV) light and darkness in the incubation chamber of Plant Pathology Department of BJRI for seven days. The presence of seed borne fungal pathogens was identified by observing their growth characteristics on the incubated seeds in blotter under stereomicroscope at 25X magnification. If there were any difficulty for identification of pathogens, temporary slides were prepared and the fungi were identified accordingly. Results were expressed as percentage of seeds infected by the pathogens. Data was analysed statistically and treatments effects were compared by Duncan’s Multiple Range Test (DMRT). Relation between seed borne fungal pathogens and germination was observed with regression equations .

Source of Information: International Journal of Plant Pathology, 5 (2): 28-53, 2014, (ISSN 1996-0719)

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Therefore, the following conclusion was drawn for storage jute seeds from the findings of this study:

• In storage period, germination, viability, moisture of seeds and health status depend upon storage containers, duration and environmental condition

So, the following recommendation may be drawn for storage and production of quality of healthy jute seeds from the findings of this study:

• Quality of jute seeds can be maintained by storage in tin pot with provax-200 treated and recommendation moisture content (9.5%)

Knowledge Management: Journal