Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Isolation and Characterization of Arsenic Resistant Bacteria from Different Environment in South-west Region of Bangladesh

M. A. Salam
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh.

M. S. Hossain
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh.

M. E. Ali
Gaco Pharmaceuticals and Research Laboratory, Dhaka-1000, Bangladesh.

M. A. Asad
Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna, Bangladesh.

M. H. Ali
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh.

Abstract/ Executive Summary:

An experiment was carried out to determine the presence and number(s) of arsenic resistant bacteria population. In this study, soil samples were collected from different contaminated sites of Khulna shipyard, Rupsha, Baghmara and Ramnagor in Khulna district of Bangladesh. Twenty arsenic resistant bacterial strains were isolated from the soil samples. They were isolated by growing them on Nutrient Broth (NB) medium impregnated with high concentration of arsenic. From them, six strains Bacillus lichnefomis (1/10), Listeria murrayi (2/9), Bacillus polymyxa (3/6), Moraxella urethralis (4/9) and Planococcus citreus (5/8) and Pseudomonas fluorescens (6/8) were finally selected and their morphological and biochemical characters studied in details. All six strains were able to tolerate very high concentration (>100 ppm) of Arsenic.The optimum pH and temperature for the growth of all six strains were 8.5 and 37°C, respectively. It was concluded that, bacteria living in arsenic free environment must posses a mechanism necessary to resist other toxic levels of arsenic.

Keywords: Biochemical, Concentration, Spectrophotometric, Environment, Bangladesh

Location: Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh

Start Date: 00-08-2007

End Date: 00-04-2008

Program Area: Risk Management in Agriculture

Commodity: Virus

Objectives:

1. To isolate bacteria which are resistant to arsenic from arsenic polluted environment,

2. To characterize the putative arsenic resistant bacteria.

Methodology:

The experiment was conducted through August 2007 to April 2008 at the Microbiology Laboratory of Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh. Soil samples were collected from different arsenic contaminated sites of Khulna shipyard (Dry soil - near the ship), Bagmara (Dry soil -side of the river, Wet soil -near the tube well, Wet soil -near the tube pond), Ramnagar (Wet soil near the tube well, Wet soil near the pond), Rupsha, Khulna in sterile polyethylene bags. Soil samples were best collected from top to 20 cm. Every time of collection hands were sterile with alcohol (95%) and then the bags were partially tilled with materials and labeled. Special care was always taken to avoid contamination of arsenic after collection the samples were brought to the laboratory carefully and put it in the freeze for immediate use. For long time preservation the sample will be storage at 4°C. Soil dilution technique is used for the isolation of arsenic resistance bacteria. At first 34 different bacterial colonies were isolated from the soil samples by culturing them in Nutrient Agar (NA) medium containing 5 ppm Arsenic. From this 6 bacterial strains were finally selected for detail study. The strains were inoculated in NA slant using a sterile loop and incubated at 37 °C for 18-27 h. After growth the slants were stored at 4 °C for short term storage and for uses. For long term preservation, bacterial growth was harvested from culture slant by sterile inoculating loop. Dense cell suspensions were prepared into 10% (v/v) aqueous glycerol in one vial and stored at 15 °C. The selected bacterial strains were morphological characterized through non-microscopic such as agar colony, agar slant, broth culture and microscopic fixed stained smear such as gram stain and spore stain modified method and biochemical characterized. Tubes of nutrient broth containing different concentration of arsenic (0, 1, 5, 10, 20, 50 and 100 ppm) were equally inoculated with fresh culture and incubated at 37 °C. After 24 h, growth on nutrient broth measured by spectrophotometric reader (600 nm). Tubes of nutrient broth were equally inoculated with fresh culture and incubated at 4, 20, 37 and 60 °C. After 24 h, growth on nutrient broth measured by spectrophotometric reader (600 nm) in four times. Tubes of nutrient broth at different pH (4.5, 6.5 and 8.5) were prepared in duplicates and after inoculation incubated at 37 °C. After 24 h, growth on nutrient broth measured by spectrophotometric reader (600 nm) in four times.

Source of Information: Research Journal of Environmental Sciences, 3(1): 110-115, 2009, ISSN 1819-3412.

Article Link (Online Journal): DOI: 10.3923/rjes.2009.110.115, URL: http://scialert.net/abstract/?doi=rjes.2009.110.115

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

All six strains were able to tolerate very high concentration (>100 ppm) of Arsenic.The optimum pH and temperature for the growth of all six strains were 8.5 and 37°C, respectively.

Knowledge Management: Journal