Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Regeneration of Plantlets from Node-derived Callus in Aegle Marmelos Corr.

M. R. Islam
Department of Genetics and Breeding, University of Rajshahi, Rajshshi 6205, Bangladesh.

S. Zaman
Department of Genetics and Breeding, University of Rajshahi, Rajshshi 6205, Bangladesh.

K. Nasirujjaman
Department of Genetics and Breeding, University of Rajshahi, Rajshshi 6205, Bangladesh.

Abstract/ Executive Summary:

In the present study, plant regeneration techniques of Aegle marmelos from nodal explant could be developed. For inducing callus, nodal segments derived from in vitro grown shoots were cultured on Murashige and Skoog (MS) medium supplemented with different compositions of phytohormones. The maximum callus formation was observed in MS medium having 0.3 mg L^-1 BA+2.0 mg L^-1 2,4-D. In all the cases, callus proliferation began at the cut ends of the explants. Optimum organogenesis and plantlet formation occurred when the calli were subcultured on medium fortified with 2.0 mg L^-1 l BA+0.1 mg L^-1 NAA. Callus-derived shoots produced roots when transferred to half strength of MS medium without any growth regulators. The rooted plant lets were successfully transplanted to soil.

Keywords: Callus node, Aegle marmelos, regeneration, Micropropagation

Location: Department of Genetics and Breeding, University of Rajshahi

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

To study the organogenesis and plant regeneration from node-derived callus in Aegle marmelos.

Methodology:

In vitro grown cotyledon-derived shoots of Aegle marmelos Corr. were excised aseptically and after removal of leaves, 7 mm long node was excised from the shoot. The nodal explants were placed horizontally on MS medium fortified with various concentrations and combinations of BA, 2, 4-D, NAA and IBA for callus induction. In all cases, callus proliferation began at the cut ends of the explants and the rate of growth was rapid. The 42 day-old proliferated calli were subcultured on MS medium containing BA alone and in combination with different composition of NAA and KIN for organogenesis and plantlet formation. For adventitious rooting half strength of MS medium without any growth regulator was used. Explants were cultured separately in 25 x 150 mm culture tube for each combination of growth hormone. All cultures were maintained in a growth room under 16 h photo period with a light intensity 2000 to 3000 lux provided by warm-white fluorescent lights at 26±2°C. All media were solidified with 6 g L^-1 agar (Carolina Biological Supply Co.) and supplemented with 30 g L^-1 sucrose for callus formation, regeneration and root induction. The pH was adjusted to 5.6 prior to autoclaving at 15 lbs pressure per square inch at 121°C temperature for 20 min. All experiments were repeated for twice.

Source of Information: Biotechnology, 6(1): 72-75, ISSN 1682-296X.

Article Link (Online Journal): DOI: 10.3923/biotech.2007.72.75, URL: http://scialert.net/abstract/?doi=biotech.2007.72.75

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The study demonstrated that plant regeneration could be achieved using callus cultures from nodal tissues of Aegle marmelos. Earlier, it has been shown that complete plant regeneration is possible from hypocotyls and cotyledons and leaf segments in this woody plant. Microbial contamination was never been a serious problem. Efficient recovery of Aegle marmelos plants through the process mentioned would facilitate storage and international exchange of germplasm and could be exploited for selecting valuable genotypes.

Knowledge Management: Journal