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Effect of Culture Media on Seed Dormancy and Callus Induction Ability of Some Wild and Cultivated Rice Genotypes

M. N. Hoque
E-31/1, Residential Area, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.

Lutfur Rahman
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.

Lutful Hassan
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.

Abstract/ Executive Summary:

An experiment was carried out at the tissue culture laboratory of the Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh in 2002.Seed dormancy status was evaluated using agar, MS media and DKW media with or without sugar (3%) in two wild (Jhora 4325 and Wild 4855) and three cultivated (Hab. Aman 2, Jagliboro and Dular) rice genotypes. Jhora 4325 showed strong seed dormancy than the wild 4855 and cultivated rice genotypes. Embryo germination percentage in the studied rice genotypes was higher in agar and MS media than DKW media. Number and percent explants with induced callus was comparatively higher in MS media in combination with 2, 4-D (1.5 mg L^-1 2, 4-D) and BAP (0.5 mg L^-1) than MS media supplemented with 2, 4-D alone. Embryogenic callus initiation capability of wild rice genotypes was higher than the cultivated genotypes. Embryo germination and callus induction were influenced by concentration of plant growth regulators, culture media and rice genotypes.

Keywords: Rice (Oryza sativa L.), Seed dormancy, Plant growth regulators, Culture media, Callus induction

Location: Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh

Start Date: 00-00-2002

End Date:

Program Area: Variety and Species

Commodity: Rice

Objectives:

To study the effect of media and growth regulators on breaking seed dormancy and callus induction ability in some selected rice genotypes representing wild and cultivated (Traditional).

Methodology:

Seeds of five rice genotypes viz., Jhora 4325, Wild 4855, Dular, Jagliboro and Hab. Aman 2 were collected from GRS-BRRI. The experiment was carried out at the tissue culture laboratory of the Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh in 2002. Dehusked seeds were surface sterilized using 0.1% HgCl₂ solution for 10-20 min and washed 4-5 times repeatedly. Agar (no sucrose), MS medium (Murashige and Skoog, 1962) (with 3% sucrose) and DKW medium (Driver and Kuniyuki, 1984) (with 3% sucrose) were used for embryo germination. Five seeds per petridish were placed with 4 replications for embryo germination and call us induction. MS medium was supplemented with 1, 1.5 and 2 mg L^-1 of 2, 4-D alone and with BAP 0.5 mg L^-1 and used for callus induction. Medium contained 0.6% Agar, 3.0 % sucrose and pH was adjusted to 5.8. Cultures were incubated at 25±2°C, 65-70% RH and 16/8 h L/D cycle with approx. 2000-3000 lux of light intensity. Germination was observed after 5 days. Data were collected on number of embryo germinated and No. of explants showing callus induction. Embryo germination percent and percent explants showing callus induction were calculated. Analyses was done using the model CRD factorial experiments with various factor and interaction effects.

Source of Information: Biotechnology, 6(1): 61-63, 2007, ISSN 1682-296X.

Article Link (Online Journal): DOI: 10.3923/biotech.2007.61.63, URL: http://scialert.net/abstract/?doi=biotech.2007.61.63

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In the present study, after adding 3% sugar in the MS media for breaking seed dormancy in rice gave better result than the culture media without sugar. Two wild rice genotypes showed better callus production ability than the cultivated genotypes. MS media supplemented with 2, 4-D in combination with BAP induced more callus. In conclusion, it is possible to break seed dormancy using tissue culture media and MS media supplemented with 0.5 mg L^-1 BAP and 1.5 mg L^-1 2, 4-D suitable for propagation of wild and cultivated rice genotypes from mature seed derived callus.

Knowledge Management: Journal