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High Frequency Plant Regeneration of a Dessert Banana Cv. Mehersagar for Commercial Exploitation

M. Z. Rahman
Department of Genetics and Breeding, University of Rajshahi, Rajshshi 6205, Bangladesh.

M. G. Sharoar
Department of Genetics and Breeding, University of Rajshahi, Rajshshi 6205, Bangladesh.

Mohammad Nurul Matin
Department of Genetics and Breeding, University of Rajshahi, Rajshshi 6205, Bangladesh.

M. H. Rahman
Institute of Biological Sciences, University of Rajshahi, Rajshshi 6205, Bangladesh.

M. Mizanur Rahman
Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh.

M. Rezuanul Islam
Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh.

Abstract/ Executive Summary:

Banana cultivar ‘Mehersagar’ is a dessert banana clones cultivated all over the country. For the purpose of the production of disease free plant lets suitable for large-scale cultivation in the field, a micro propagation protocol based on multiple shoot regeneration has been established. Highly proliferative cluster of multiple shoots are obtained by culturing shoot tips from ex vitro grown healthy suckers on MS medium with different concentrations and combinations of cytokinins (viz., BAP and KIN), auxins (viz., IBA, NAA and IAA) and coconut water (cw). The efficacy of these supplements on the rate of shoot multiplication was tested. Best results were obtained on MS+0.5 mg L^-1 BAP+0.5 mg L^-1 KIN+13% cw, where highest average number of shoots per explant was 8.2±0.236. When individual shoot lets were separated and implanted in half strength MS medium with various concentrations of auxins, the better root formation were noted at 1.0 mg L^-1 IBA with 6.10±0.359 roots per shoot. Then the rooted plant lets were transferred to poly bags and about 90% of the acclimatized plant lets were survived when transferred to open field.

Keywords: Dessert banana, Micropropagation, Callus, Regeneration

Location: Puthia upazila under Rajshahi District of Bangladesh

Start Date: 00-00-2003

End Date: 00-00-2004

Program Area: Variety and Species

Commodity: Banana

Objectives:

To formulate the appropriate protocol for better shoot multiplication as well as rooting of banana cv. Mehersagar.

Methodology:

Banana (Musa sp.) cultivar “Mehersagar” growing in the cultivated field in Puthia upazila under Rajshahi District of Bangladesh was used as the source material for obtaining shoot tips and research has been done in the year 2003-2004 at Biotechnology Laboratory, Institute of Biological Sciences, Rajshahi University, Bangladesh. Small healthy sword suckers were carefully removed from field-grown banana. Then the older leaves and extraneous corm tissue were carefully removed with a stainless knife. Shoot tips containing several sheathing leave bases enclosing the auxiliary bud and subjacent corm tissue and measuring about 5-6 cm in length were isolated. These shoot tips were firstly washed in running tap water for 40 min and then with teepol for 15-20 min. All traces of teepol were removed by washing several times with autoclaved distilled water. Finally, the explants were placed on inoculation chamber and treated with 0.1% MgCl₂ for 14 min. followed by washing several times with sterile distilled water to wash out any trace or MgCl₂. After surface sterilization they were trimmed 2-3 mm from both ends and the outer sheath was peeled off gradually. Intact shoot apex and one or more pairs of leaf primordia together with 2-3 mm in size including rhizomatous base were selected for inoculation. The individual explants were cultured in agar gelled MS nutrient medium containing different concentrations and combination of cytokinins, auxins and coconut water. For rooting, half strength MS with auxins was used in all the media. The media were adjusted to pH 5.8 and autoclaved for 20 min at 121°C under 1.1 kg cm^-2 pressures. Cultures were incubated at 25±10°C under 16 h photo period with light intensity of 2000-4000 lux.

Source of Information: Biotechnology, 5(3): 296-300, 2006, ISSN 1682-296X

Article Link (Online Journal): DOI: 10.3923/biotech.2006.296.300, URL: http://scialert.net/abstract/?doi=biotech.2006.296.300

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In the present study, we observed 1.0 mg L^-1 IBA in half strength MS agar gelled medium was optimal for root induction. Acclimatized rooted shoots were planted in small polythene bags or pots containing sterile sand, soil and humus (1:2:1). The plant lets were covered by transparent polythene sheet to maintain high humidity and within 15-20 days new leave emerged from about 90% of the plant lets that resumed new growth. After 60-70 days, the plants were transplanted in the open field where 90% plants survived and grew satisfactory. The protocol, that has been established, holds great promise for commercial cultivation of disease free planting materials for the popular cultivar of “Mehersagar”.

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