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Lateral Bud Culture of Papaya (carica Papaya) for Clonal Propagation

M. M. Rohman
Laboratory of Plant Stress Responses, Department of Plant Sciences, Faculty of Agriculture, Kagawa University, Japan.

Md. Nazrul Islam
Horticulture Research Centre, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.

Md. Shameul Alam
Department of Biochemistry and Food Sciences, Faculty of Agriculture, Kagawa University, Japan.

Munshi Rashid Ahmad
Horticulture Research Centre, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.

Tapan Kumar Paul
Regional Agricultural Research Station, Bangladesh Agricultural Research Institute, Hathazari, Chittagong, Bangladesh.

Abstract/ Executive Summary:

An experiment was conducted at the Tissue Culture Laboratory of Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur during June 2004 to March 2005. In vitro culture of lateral buds of field grown mature plants were tested in MS medium supplemented with different concentrations of BAP and NAA. Seasonal endophytic contamination was suppressed by shaking propagules for 2 h in 300 mg L^-1 rifampicin before surface sterilization. Maximum survival rate was found in MS medium supplement with 1.0 mg L^-1 BAP plus 0.20 mg L^-1 NAA. The highest number of shoots was produced in MS medium containing 0.50 mg L^-1 BAP and 0.20 mg L^-1 NAA. Both the highest multiplication rate and the longest shoot were found in MS medium supplemented with 0.50 mg L^-1 BAP plus 0.20 mg L^-1 NAA. On the same treatment an increasing trend was observed in multiplication rate of shoot up to 5th subculture which decreased thereafter. First subculture produced the highest shoot length which decreased with the increase in number of subculture. Half strength MS medium supplemented with 1.0 mg L^-1 IBA was found as the best treatment to produce root in the culture media. Rooted plantlets were transplanted successfully.

Keywords: In vitro, Carica papaya, Lateral bud, Clonal propagation

Location: Tissue Culture Laboratory of Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur

Start Date: 00-06-2004

End Date: 00-03-2005

Program Area: Variety and Species

Commodity: Papaya

Objectives:

To establish a suitable protocol for rapid plant regeneration in vitro from mature papaya plants.

Methodology:

The experiment was conducted in the Tissue Culture Laboratory of Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur during June 2004 to March 2005. Lateral buds consisting of the top young unexpended leaves collected from field grown mature plants were used as explants in this study. The buds were shaken in an antibiotic, rifampicin (RIF) at 300 mg L^-1 to suppress the bacterial contamination. The buds were then cleaned with soap water and treated for 7 min with mercuric chloride (0.1%) containing a few drops of Tween-20. This was followed by 3-4 washings with sterile distilled water. The explants were agitated in sterile distilled water for 2-3 h in horizontal shaker at 160 rpm before inoculation to minimize the flow of latex into medium. The MS (Murashige and Skoog, 1962) was used as basal medium with different concentration of BAP (0.1, 0.2, 0.3, 0.4, 0.5, 1.0, 2.0, 3.0 and 4.0 mg L^-1) with different concentration of NAA (0.10, 0.20 and 0.30 mg L^-1). Individual elongated shoots were excised carefully and transferred to half strength MS media with Indole-3-Butyric Acid (IBA) at different concentrations (0.10-0.5 mg L^-1). The pH of the media was adjusted at 5.8 prior to autoclave and 8 g L^-1 agar was added to solidify the media. The cultures were kept under approximately 2000 lux fluorescent light at 25±1°C with 16 h photo period.

Source of Information: Biotechnology, 6(3): 339-343, 2007, ISSN 1682-296X

Article Link (Online Journal): DOI: 10.3923/biotech.2007.339.343, URL: http://scialert.net/abstract/?doi=biotech.2007.339.343

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Although the survival rate of the plantlets were low, its improvement seemed possible for commercial purposes and the present study indicated the possibility of producing papaya plants from mature plants through later bud culture. In the culture media, formation of calli was a great problem to develop root and further study would be needed to resolve the problem.

Knowledge Management: Journal