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Production of Synthetic Seed by Encapsulating Asexual Embryo in Eggplant (solanum Melongena L.)

A. K. M. N. Huda
Institute of Biological Sciences, University of Rajshahi, Rajshshi 6205, Bangladesh.

M. A. Bari
Institute of Biological Sciences, University of Rajshahi, Rajshshi 6205, Bangladesh.

Abstract/ Executive Summary:

The study was performed at the Institute of Biological Sciences, University of Rajshahi, Bangladesh in 2005 to investigate on the technology of synthetic seed production using somatic embryos in two varieties e.g Loda and China of eggplant. To standardize a media composition of artificial endosperm of synthetic seed, different concentrations and combinations of phytohormones with MS media were used in seed bead to achieve optimum germination rate on MS0 media. Among the different concentrations and combinations, MS+1.0 mg L^-1 BAP+0.1 mg L^-1 GA₃ gave the highest germination rate and it was 81% for Loda and 70% for China variety. The influence of storage at 4°C and 0°C temperature on germination rate was also examined. This investigation indicated that, synthetic seed could be stored at 4°C temperature for 45 days.

Keywords: Synthetic seed, Sodium alginate, Somatic embryo, Encapsulation, Eggplant, Bringal

Location: Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh

Start Date: 00-00-2005

End Date:

Program Area: Seed Technology

Commodity: Brinjal

Objectives:

To establish an efficient protocol for production of synthetic seeds of eggplant/bringal

To maintain proper genotype of resultant plant and preservation of eggplant/bringal

Methodology:

This research was conducted at the Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh in 2005. In this experiment, two varieties of Solanum melongena viz., Loda and China were used. The seeds of two varieties were cultured on MS0 medium for seedlings to have easy supply of cotyledon pair. Cotyledons were used as explants and cultured on callus inducing medium (2.0 mg L^-1 NAA+0.05 mg L^-1 BA) for callus induction. Best calli derived from cotyledonary explants of the two varieties were sub cultured separately on MS media having different concentrations and combinations of phytohormone for somatic embryogenesis. Somatic embryos formed within 7-28 days were used as explant/materials for preparing synthetic seed in the subsequent experiments. MS medium was used as the basal medium supplemented by phytohormone and carbon sources. Sodium alginate was added to the medium and mixed well to make a homogenous solution. The explants were taken in a beaker containing alginate solution and the explants were dipped into the alginate solution. Explants dipped in alginate solution were taken up by a forcep and placed to the beaker of CaCl₂ and each explant then became a hard ball encoded by alginate. To examine the germination rate, the synthetic seeds were cultured on MS0 media and data were recorded. On the other hand, to examine their viability range, the synthetic seeds were stored at 4°C and 0°C temperature in refrigerator for 15, 30, 45, 60, 90 and 120 days. Following storage in refrigerator under respective days regime seeds were cultured on MS0 medium.

Source of Information: International Journal of Agricultural Research, 2(9): 832-837, 2007, ISSN 1816-4897.

Article Link (Online Journal): DOI: 10.3923/ijar.2007.832.837; URL: http://scialert.net/abstract/?doi=ijar.2007.832.837

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The results of this study demonstrated that seeds could be germinated on MS media or even on agar beds containing distilled water which reduced the labor and operational cost. Thus,it has opened a new area of advance research for developing the conservation strategies for eggplant genetic resources by the application of biotechnological tools like chilling and Cryopreservation.

Knowledge Management: Journal