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Brucellosis in Sheep and Goat of Bogra and Mymensingh Districts of Bangladesh

Md. Siddiqur Rahman
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

Md. Ferdous Ali Hahsin
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

Md. Shamim Ahasan
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

Moon Her
OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency, Ministry of Food, Agriculture, Forestry and Fisheries, Anyang 430-757, Korea

Jeong Yeong Kim
OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency, Ministry of Food, Agriculture, Forestry and Fisheries, Anyang 430-757, Korea

Sang Il Kang
OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency, Ministry of Food, Agriculture, Forestry and Fisheries, Anyang 430-757, Korea

Suk Chan Jung
OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency, Ministry of Food, Agriculture, Forestry and Fisheries, Anyang 430-757, Korea

Abstract/ Executive Summary:

Brucellosis is the most important bacterial disease of livestock in Bangladesh. The present study was conducted to determine the seroprevalence of brucellosis in goat and sheep in Mymensingh and Bogra districts of Bangladesh using slow agglutination test and Rose Bengal test as screening test and indirect enzyme linked immunosorbent assay as confirmatory test. Questionnaire based data on age, gender, area, client’s complaint, number of animals in herds, disease history, reproductive problems such as abnormal uterine discharge, abortion or previous abortion in sheep and goat and their reproductive diseases were recorded. A total of 200 sera samples were collected from 80 sheep and 120 goats. The prevalence of brucellosis in goat was 2.50% and 1.25% in sheep. Positive reactors were only detected in female of both goat and sheep. In this study, there existed a significant association among abortion and the prevalence of brucellosis (p < 0.01). The prevalence of brucellosis in sheep and goat in Bangladesh is not negligible, and it is therefore worth considering the adoption of preventive measures.

Keywords: Bangladesh, Brucellosis, Goat, Seroprevalence, Sheep

Location: Mymensingh and Bogra districts of Bangladesh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Goat, Sheep

Objectives:

The present study was carried out for diagnosis of brucellosis in sheep and goats by the indirect enzyme linked immunosorbent assay (I-ELISA) in Mymensingh and Bogra districts of Bangladesh.

Methodology:

A total of 200 blood samples were collected from sheep and goats of 1~3 years old in Mymensingh and Bogra districts of Bangladesh. Among them goat (50) and sheep (35) sera were collected from Mymensingh and goat (70) and sheep (45) samples were collected from Bogra. The study recorded some clinical, epidemiological and reproductive information. The questionnaire based data on age, gender, area, client’s complaint, number of animals in herds, disease history, reproductive problems such as abnormal uterine discharge, abortion or previous abortion in sheep and goat and their reproductive diseases were recorded. All blood samples were processed for sera preparation and were tested with slow agglutination test (SAT) and Rose Bengal test (RBT) as screening test. I-ELISA was used for confirmatory diagnosis. RBT was performed according to the procedure as described by Office International des Epizooties, France. The test serum samples and Rose-Bengal antigen were kept 1 h in room temperature before beginning of the test. The result was considered positive when there was any degree of agglutination noticeable and absence of agglutination considered as negative. SAT was carried out with EDTA. Briefly the slow agglutination test was performed in “U” bottom 96 well microplate and tested to duplicate well by 100 μL serial dilution (initial dilution 1/6.25) and then 100 μL antigen was added. The plate was incubated at 37^oC for 24 h (± 4 h) for reading. Agglutination reaction was observed by using a magnifying mirror against illumination source. Reading was taken on the basis of this protocol and the standardization we performed (75% agglutination of the OIEISS). I-ELISA was performed according the protocol and suppliance provided by the ELISA kit (Svanova Biotech AB, Sweden) as per manufacturer instruction. The questionnaire-based data was processed by Microsoft Excel and Static, and results were statistically analyzed for interpretation.

Source of Information: Korean J Vet Res(2011) 51(4) : 277~280

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present investigations revealed that the overall seroprevalence of brucellosis in goat was 2.50% which is similar to or lower than 2.33~14.57% reported by others. Sex, Age, location and living condition influenced the sero prevalence of brucellosis.

Knowledge Management: Journal