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Alteration of Cellular Nutritional Elements and Nucleic Acids of Papaya Leaves Infected with Seven Symptomatic Isolates of Prsv-p

H. Rahman
Department of Plant Pathology, Patuakhali Science and Technology University, Dumki, Patuakhali, Bangladesh.

M. M. Alam
Department of Genetics and Plant Breeding, Patuakhali Science and Technology University, Dumki, Patuakhali, Bangladesh.

A. M. Akanda
Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur, Bangladesh.

Abstract/ Executive Summary:

The diverse nature of shift in the amount of cellular nutritional elements and nucleic acids as affected by the infection of different isolates of Papaya Ring Spot Virus-Papaya strain (PRSV-P) was studied in this experimentation. The symptomatic isolates used in the present study were mild mosaic, mosaic, fern leaf, severe mosaic, vein clearing, leaf distortion and chlorotic leaf spot. Parameters measured to quantify the alteration of cellular components comprised different nutritional cellular elements (C, N, P and K) and nucleic acids (DNA and RNA). In all occasion of measured parameter, the highest alteration was found in leaf distortion. Almost in all cases the lowest effect was determined with the Mild Mosaic infected plants followed by the mosaic, severe mosaic, vein clearing, chlorotic leaf spot, fern leaf and leaf distortion. Infection of seven different symptomatic isolates of PRSV-P created striking and responsive variability in all parameters studied. And the dependency of the cellular nutritional elements and nucleic acid on seven symptomatic variants of PRSV-P was found significantly correlated in respect to the corresponding yield.

Keywords: PRSV-P, Symptomatic Isolates, Cellular components, Nucleic acids

Location: Department of Plant Pathology, Patuakhali Science and Technology University, Dumki, Patuakhali

Start Date:

End Date:

Program Area: Pest Management

Commodity: Papaya

Objectives:

To picturing the diverse alteration in the cellular nutritional elements and nucleic acids from the infection of seven different symptomatic isolates of PRSV-P on papaya in Bangladesh.

Methodology:

Papaya plants infected with Papaya ring spot virus- Papaya strain showing seven different types of symptoms named by the seven symptomatic isolates were selected and tagged. Leaf samples were collected from the healthy and infected plants from the similar position and similar age showing distinct symptoms to maintain the sample homogeneity. Samples were also collected at three different growth stages of plants (Early = before fruit setting, mid = fruit setting to first ripening and late = first ripening onward) infected with each symptomatic isolates. The healthy and infected plants were frequently checked for PRSV-P infection using mechanical sap inoculation and DAS-ELISA methods, respectively. A double beam spectrophotometer (Model No. 1200-20, Hitachi) was used to measure the absorbance value/optical density (OD) at weave length given by the protocol when it was required. Organic carbon was estimated by weight dilution method. Total nitrogen was determined by Kjeldahl method using CuSO₄ and K₂SO₄mixture (1:9) as catalyst. The total phosphorus content was estimated by Perchloric acid digestion method. The content of potassium was measured by following perchloric acid digestion method for mineral elements. Content of nucleic acids (DNA and RNA) was determined by the method. Change in structural cellular nutritional elements (organic C, N, P and K) and nucleic acids (RNA and DNA) both for healthy and PRSV-P infected leaf samples were taken in every quantifying analysis. Each of the cellular elements were analyzed from 10 samples. Variation among the symptomatic isolates was measured using DMRT (Duncan`s Multiple Range Test).

Source of Information: International Journal of Agricultural Research, 3(3): 219-226, 2008, ISSN 1816-4897

Article Link (Online Journal): DOI: 10.3923/ijar.2008.219.226; URL: http://scialert.net/abstract/?doi=ijar.2008.219.226

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The results of the study with PRSV-P infecting papaya conclusively differentiated the identity of seven different symptomatic isolates of PRSV-P as distinctly different variants, each varied from the other in altering the cellular components in their respective infected papaya leaves. Since the virus PRSV-P studied in the present experiment is a RNA virus. The alteration so far obtained in nucleic acids and in ratio of nucleic acids in the papaya leaves infected with seven different symptomatic isolates of PRSV-P seemed to be consistently varied depending on the variants identified on the basis of symptoms induced in papaya.

Knowledge Management: Journal