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Effect of Different Pathotypes of Bipolaris Sorokiniana on Leaf Blight Severity and Yield Contributing Characters of Wheat Cv. Kanchan Inoculated at Maximum Tillering Stage

F. Ahmed
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

F.M. Aminuzzaman
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh..

Hossain
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

A total of representatives eleven isolates of six different pathotypes of Bipolaris sorokiniana were compared for their effect on leaf blight severity and yield contributing characters by inoculating plants of wheat cv. kanchan at maximum tillering stage under control condition in pot trials. The pathotypes differed among themselves in respect of leaf blight severity that scored from 61 to 81. In case of yield contributing characters, the pathotypes did not differ among themselves that indicates all available pathotypes of B. sorokiniana in Bangladesh are of equal value in respect of wheat cultivation.

Keywords: Wheat, Bipolaris sorokiniana, Pathotype, Inoculation., Maximum tillering stage

Location: Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh

Start Date: 00-00-1999

End Date: 00-00-2000

Program Area: Crop-Soil-Water Management

Commodity: Wheat

Objectives:

To compare the effect of different pathotypes of BipoIaris sorokiniana on leaf blight severity and its influence on yield contributing characters when plants get infection at maximum tillering stage.

Methodology:

The experiments were conducted during the period of 1999 to 2000. The representative isolates of six different pathotypes of Bipolaris sorokiniana were collected from the Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh. The pure culture of the isolates of B. sorokiniana were transferred to slants and stored in refrigerator at 5^oC for further research work following the method. The soil was collected from the Agronomy Farm, Bangladesh Agricultural University (BAU), Mymensingh. The compost was collected from the Dairy Farm, BAU, Mymensingh. The collected soil and compost (4:1) were mixed thoroughly and the earthen pots (20 cm dia) were filled with the mixture of soil and compost. Wheat seeds (10 Pot^-1) were sown in pots and finally 5 seedlings were allowed to grow. Plants were watered whenever necessary. Three pots were used for each treatment. Eleven representatives selected isolates of six pathotypes of BipoIaris sorokiniana were transferred from stock cultures and were grown PDA in petriplates for 10 days at room temperatures (22-24°C). Then the petriplates were kept under NUV light for 5 days for formation of maximum conidia. Conidial suspension were prepared with distilled water by collecting the conidia from the agar surface of PDA. The conidial suspension was then filtered through cheese cloth to remove mycelial fragments and lumps of agar. The conidial suspension was then prepared to 10⁴ml conidia ml^-1 following the ClMMYT method. The inoculation was done by spraying conidial suspension at maximum tillering stage of wheat plants with a spore suspension (10⁴ conidia ml^-1) using a self compressed sprayer. Just after inoculation, the pots were covered with previously moistened polyethylene bags for 36 h for maintaining moisture. After removing the polyethylene bags the leaf surfaces were exposed but watering of pots were continued for supplying moisture to the seedlings. Control treatment was maintained by protecting the plants with Tilt (0.1%) following spray method as Tilt was found most effective for controlling Bipolaris leaf blight of wheat. Bipolaris leaf blight (BpLB) severity was recorded after 15 days of flowering using (BpLB terminal score), double digit scale proposed by CIMlMYT. Yield contributing characters were determined following the method. Data were collected from each plants on Plant height (cm), ear length (cm), number of grains/ear, healthy and diseased grains/ear, grading of seeds (0-5 scale) following CIMMYT method, weight of grains/ear (g), 1000 grains weight (g) and yield of grains/pot (g).

Source of Information: Pakistan Journal of Biological Sciences 6 (7): 693-696, 2003 (ISSN 1028-8880)

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From this study it has clearly been found that the pathotypes of Bipolaris sorokiniana showed similar effect on grain formation though variation in respect of leaf blight were severity recorded. This leaf blight disease is of serious concern to wheat growers as well as wheat researchers in the country. This information is highly required to wheat Pathologist as well as wheat growers is the country for developing a new wheat variety in Bangladesh when they will use B. sorokiniana as a test pathogen.

Knowledge Management: Journal