Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Microbial Study of Some Milk with Special Reference to Coliform Bacteria

Bejon Kumar Bhowmick
Microbiology Laboratory, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh.

Mihir Lal Saha
Microbiology Laboratory, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh.

Mahabubar Rahaman Khan
Microbiology Laboratory, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh.

Abstract/ Executive Summary:

Milk products namely, Cheese, Borhanii and Whey samples of Dhaka Metropolitan City studied for the justification of the quality and hygiene. The range of Coliform bacteria were ranged from 5.24 x 10⁵ to 0.5x10⁴ CFU in Borhanii, 6 x 10³ to 5.0 x 10³CFU in Cheese and 2.98x10⁵ to 1.8x10⁴ CFU in whey respectively in our samples. Antibacterial sensitivity tests were carried out with five different antibiotics against bacterial isolates. All isolates were resistant to Streptomycin and sensitive to Gentamycin. The majority of the isolates were sensitive to Chloramphenicol and Tetracycline. Four isolates were resistant to Ampicillin.

Keywords: Cheese, Borhanii, Whey, Antibiotics, Coliform, Bacteria, Milk products

Location: Dhaka Metropolitan City.

Start Date:

End Date:

Program Area: Quality and Nutrition

Commodity: Milk

Objectives:

To assess the microbial pattern of the Coliform Bacteria in milk/milk products
To document the result for dissemination as a matter of safe food for all

Methodology:

Samples of milk were collected from different community centers, hotel, restaurants and footpath vendors of the Dhaka Metropolitan City. The microbial colonies were taken by dilution factor (1:100) plate procedure. Membrane filter technique was done by the machine (Memmert Gmbh Co) and Mac.Conkey Agar medium was used for counting Coliform bacteria colonies. The dilution sample was prepared sterile conical flask under pH controlling and shaken vortex stirrer (VF1 Jurk and Kunkel Gmbh and Co Germany). Serial diluted samples were poured in duplicated and sterilized plates and then were incubated at 37°C in an incubator (Memmert Gmbh Co) maintaining a high level of humidity inside for 24 h. Lactose fermentation test was done at 44°C for 24 h to identify fecal coliforms. Nine isolates of bacteria were selected on the basis of their morphological and biochemical characteristics and nature of arrangement through microscopic studies after Gram staining with Nikon Microphot microscope (Japan). The method of Eklund and Lankford (1967) was followed regarding this staining. Among the isolates, three isolates were selected from cheese, one from borhani and five from whey. Nutrient agar, blood agar and macconkey agar were used as media. The organisms were tested for antibiotic sensitivity. Nutrient agar plates were used for this purpose. Using cotton swab, test organisms were inoculated. Different types of antibiotic disks such as tetracycline (TE-30) ampicilin (AMP-25), chloramphenicol (C-30) and streptomycin (S-25) were placed on the inoculated plates. After 24 h of incubation, inhibition zones were measured. To determine the sensitivity pattern, measured diameter of the inhibition zones were compared to that of standard.

Source of Information: International Journal of Dairy Science, 1(1): 57-62, 2006, ISSN 1816-4935.

Article Link (Online Journal): DOI: 10.3923/ijds.2006.57.62; URL: http://scialert.net/abstract/?doi=ijds.2006.57.62

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The experimental results presented here emphasized the need for establishing an efficient quality control to safe guard human health as well as careful selection of antibiotics for drug resistant strains.

Knowledge Management: Journal