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Effect of Naci on Germination, Plumule and Radicle Growth of Some Modern Rice Genotypes

M Z Alam
Senior Scientific Officer
Bangladesh Rice Research Institute (BRRI), Gazipur 1701, Bangladesh.

T Stuchbury
Lecturer
Department of Agriculture and Forestry, MacRobert Building, University of Aberdeen, 581 King Street, AB24 5UA, UK.

R E L Naylor
Professor
Department of Agriculture and Forestry, MacRobert Building, University of Aberdeen, 581 King Street, AB24 5UA, UK.

M J Islam
Principal Scientific Officer
Bangladesh Rice Research Institute (BRRI), Gazipur 1701, Bangladesh.

Abstract/ Executive Summary:

Salt tolerance at germination and early stages of seedling growth was examined in eight modem rice genotypes and compared with salt-tolerant check variety, BR23 and salt-sensitive check variety, IR8 at 0, 50, 100, 150, 200 and 250 mM salinity. Germination percentage decreased significantly at 100-250 mM salt concentrations and there were also significant differences between the cultivars. Germination was mainly affected at and above 150 mM salt concentrations and cultivar differences were clear at 200 and 250 mM. In BR5331-93-2-8-4, BR5778-156-1-3, BR5828-11-1-4 and BR23 more than 75% germination even at 250 mM salt were recorded. Germination rate decreased with increasing NaCl concentrations which differed between genotypes. Rice genotypes were substantially more tolerant to salt during germination than in early stages of seedling growth. Salt solutions significantly reduced plumule and radicle growth. The plumule growth was more affected than radicle. Seedling fresh weight decreased, whereas seedling dry weight increased with increasing salinity. The variation between rice genotypes in germination and in early seedling growth performances might be genetic or related to seed quality.

Keywords: Salt tolerance, Germination, Plumule, Radicle, Rice

Location:

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Fertilizer, Rice

Objectives:

To determine the level of salt tolerance at germination and early stages of seedling growth of rice
To compare the performance of six advanced salt-tolerant breeding lines with varieties known to be tolerant or susceptible

Methodology:

Ten rice (Oryza sativa L.) genotypes such as BR1192-2B-35 (V₁), BR5331-93-2-8-3 (V₂), BR5331-93-2-8-4 (V₃), BR5778-156-1-3 (V₄), BR5828-11-1-4 (V₅), BR5842-15-4-8 (V₆), BR23, BRRI dhan29, BRRI dhan31 and IR8 were assessed in this experiment of which six genotypes were salt-tolerant advanced breeding lines (V₁- V₆). Reaction to salt of BRRI dhan29 and BRRI dhan31 are not known. BR23 was included as a salt-tolerant check and IR8 as salt-sensitive variety. Seed germination was assessed using four replicates of 25 seeds. Prior to germination test, seeds were surfaced sterilized with 1% sodium hypochloride solution for one minute and rinsed with distilled water in sterile vials for two one- minute periods. Seeds were placed in line on an 80- x 18.5 cm paper towel, folded to give two layers and pre-moistened with either 30 ml of distilled water or salt solution. Seeds were covered by another fold of 40 cm long paper towel, pre- moistened with 10 ml of respective experimental solution. Each towel was then rolled and partially immersed in a beaker containing 10 ml of appropriate treatment solution. Four beakers of similar treatment solution were placed on a small plastic tray which was then kept in a tightly closed polythene bag and placed for germination at 21 ± 1°C in the dark. Germination was recorded every day for 9 days. Seeds were considered germinated when both the plumule and radicle were more than two mm, The plurnule and radicle length of 10 randomly selected seedlings in each replicate was measured at nine days. Dry weights after oven drying at 70°C for 72 hours of randomly selected 20 seedlings also at 9d were obtained including remnants. Mean germination time (MGT or T₅₀) was calculated according to Younsheng and Sziklai (1985) as MGT = Œnd/N (where, n is the number of germinated seeds on each day, d is the number of days from the beginning of the test and N is the total number of germinated seeds). Germination rate (GR) was calculated as GR = 1/T₅₀. All percentage germination data were transformed to arc sine values before statistical analysis. Plumule and radicle lengths were analysed using analysis of covariance to allow for the different germination times and therefore, different opportunities for growth of the various treatments. The data were analysed using analysis of variance in GENSTAT. The statistical significance of differences between pairs of treatments was determined by Student's t-test.

Source of Information: Bangladesh Rice J. 12 (1 & 2) : 45-50, 2007

Article Link (Online Journal):

Funding Source:

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Achievement/Findings:

The results of this study could find germination and early seedling growth stage to be influenced by rice genotypes. Further, It was indicated that, salt tolerance during germination might differ from that at other growth stages in rice

Knowledge Management: Journal