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Effect of Flushed Feeding and Age on Estrus Synchronization and Conception Rate of Holstein × Local Crossbred Cows After Using Analogue GnRH and Prostaglandin F2? Hormone

M. Sydur Rahman
Remount Veterinary and Farm Core Depo, Savar, Dhaka, Bangladesh.

M. Kabirul Islam Khan
Department of Genetic and Animal Breedinge, Chittagong Veterinary and Animal Science University, Khulshi, Chittagong ? 4225, Bangladesh.

T. Bilkis
Department of Genetic and Animal Breedinge, Chittagong Veterinary and Animal Science University, Khulshi, Chittagong ? 4225, Bangladesh.

Abstract/ Executive Summary:

A study was conducted to understand and determine the effect of flushed feeding on estrus synchronization and conception rate of Holstein X Local crossbred cow’s after using the GnRH (fertagyl) and PGF₂α hormone. As a representative sample, 20 Holstein X Local crossbred cows were chosen from the Chittagong Cantonment Military Dairy Farm,Bangladesh and the experimental work was carried out for 60 days. Ten cows were flushed with high energy feed (10.5 mega Joule metabolisable en ergy/kg feed dry matter) and considered as treated group and 10 cows was allowed for normal feeding. Both the control and treated group cows were injected intramuscularly by the GnRH and PGF₂α hormone. After injection of these hormones, 100% cows from both groups showed estrus. Fixed time artificial insemination (AI) was done and the cows were diagnosed in pregnancy after 60 days of AI. It was observed that 20% cows were conceived after AI from control group, but treated group cows showed more conception/pregnancy rate (40%). After using the hormones, age of the cow was observed not to influence in estrus synchronization, but in case of conception rate, it was observed that, as obvious, the younger cows showed greater response than the older ones.

Keywords: Artificial insemination, Dairy cows, Conception rate, Crossbred, Estrus synchronization, Hormones.

Location: Military Dairy Farm,Chittagong Cantonment, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Cow

Objectives:

(i) To understand the effect of flushed feeding on estrus synchronization and conception rate of crossbred cow’s on using hormone.

(ii) To investigate on the role of age on conception rate of flushed Holstein X Local crossbred cows after using hormones.

Methodology:

The research was conducted at the Military Dairy Farm of Chittagong Cantonment of Bangladesh and the duration of the experiment was 60 days. In this experiment, the effect of short term (two weeks) flushed feeding on estrus synchronization and conception rates of crossbred cows (50% L X 50% F) after using the GnRH (fertagyl) and Prostaglandin F₂α (Dinoprost) hormone. A representative sample of 20 Holstein X Local crossbred (50 to 62.5% exotic blood levels) cows of 2 and 3 lactations were chosen ba sed on their production performance, physical appearance, pedigree record, body condition score, health condition (no diseased, no ovarian cysts pre- sent) and good reported fertility. The age of the selected cows was between 50-72 months. The 20 selected cows were divided into two groups: control and treatment groups, each group having ten cows. Control group provided only hormonal treatment with normal ration of the farm and treatment group provided nutritional flushing (extra 01 kg concentrate and ad libitum fodder, having 10.5 MJ metabolisable energy per kg feed dry matter) for two weeks before hormonal treatment. After hormonal treatment the cows of the treatment group were provided normal ration as given in the control group. Green grasses were provided ad libitum throughout the period including flushing period. All cows were treated with anti- helmintics before the start of this experiment to control the parasitic infestation. The experimental cows were maintained install feeding system. Each cow was intramuscularly injected with gonadotopin releasing hormone (GnRH) analogue (Fertagy 1) manufactured by Intervet, according to the instructions provided by the manufacturer in a dose of 250 μg on day 1 and 10 mg Prostaglandin F₂α (Dinoprost) on day 8 followed by 250 μg GnRH analogue after 48 hours on day 10 was administrated. At the end of day 10 the cow exhibits sexual desire by valleys frequently and accepting the males to mount on her; furthermore the vaginal and cervical mucus was visible and hanging from the vulva or wrapped around the tail. In the early morning of day 11 (during 12-18 hour of heat) the first AI was done with frozen thawed semen were collected from Central Cattle Breeding Station (CCBS), Savar, Dhaka then after 12 hour 2^nd AI was done. The cow was observed for return of heat and pregnancy (conception) was diagnosed after 60 days of AI through rectal palpation. The estrus and conception rate of flushing Holstein × Local crossbred cows were recorded according to the age of the cows to know the age effect on estrus synchronization and conception rate. Results were expressed as the percentage and data was analyzed using the SPSS software. Least significant different (lsd) test was done for comparison the significant means difference at 5% level of significance

Source of Information: Iranian Journal of Applied Animal Science (2014) 4(3), 493-497

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It could be concluded tha, the cows exhibit 100% estrus after administration of the combination of GnRH and prostaglandin F₂α hormone. The conception rate after 1st time artificial insemination (AI) and 2nd time AI for the flushed feed group was higher than the control group. Age matter did not influence in inducing heat, but the conception rate was affected. Estrous synchronization could be a useful tool in the reproductive management of a cow herd. However, if proper levels of nutrition, body condition and health are not maintained, the program is likely to be in vein.

Knowledge Management: Journal