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Protocol Establishment for Micro Propagation and in Vitro Callus Regeneration of Maulavi Kachu (xanthosoma Sagittifolium L Schott.) from Cormel Axillary Bud Meristem

K. K. Paul
Institute of Biological Sciences, University of Rajshahi, Rajshshi 6205, Bangladesh.

M. A. Bari,
Institute of Biological Sciences, University of Rajshahi, Rajshshi 6205, Bangladesh.

Abstract/ Executive Summary:

The effects of various growth regulators on organogenesis from cormel axillary bud meristems of cultivated species of Maulavi kachu (Xanthosoma sagittifolium (L). Schott. were investigated. Highest percentage (65%) direct regeneration takes place within 25 days of culture but significant number of shoot per plant and length of shoot did not found significantly. Highest percentage (90%) calluses were initiated from axillary buds on MS medium supplemented with 1 mg L^-1 BAP+2.0 mg L^-1 NAA. Light green, greenish friable or semi friable calluses were found. The highest fresh weight of callus was 11.60 g. These calluses were cultured into MS media containing with 3 mg L ^-1Kn + 2 mg L^-1 NAA to induce plant lets regeneration. Highest percentage (86%) regeneration was observed with single or aggregated green shoot buds. The average number of green shoot, daughter buds and plant lets were 2.60 and average length of shoots were 7.0 cm with the same medium. Highest percentage of root induction and proliferation were also found into media containing 0.4 mg L^-1 IAA and number of roots and root length of regenerated plant lets were 7.90 No. and 6.90 cm, respectively. Plant lets were then established with normal and natural fertile soil on plastic pot showed best performance in acclimatization.

Keywords: Callus, Maulavi Kachu, Organogenesis, Meristem, Acclimatization, Regeneration,

Location: Institute of Biological Sciences, Rajshahi University, Bangladesh.

Start Date: 00-00-2004

End Date: 00-00-2006

Program Area: Crop-Soil-Water Management

Commodity: Aroids

Objectives:

To investigate the effects of various growth regulators on organogenesis from cormel axillary bud meristems of cultivated species of Maulavi kachu

Methodology:

In the present study, axillary bud meristem was used as explants of Maulavikachu; which were collected from field grown (2004-2006) cultivated species of the experimental research farm of Institute of Biological Sciences, Rajshahi University, Bangladesh. The collected axillary bud meristem attached with cormels were washed under running tap water. Then terminal sprouts were excised from cormels and surface sterilized with 1-3 drops of tween-80 and Savlon per 100 mL water for 15 min followed by several washing with sterilized distilled water.Surface sterilization was carried by dipping 0.10% HgCl₂ by gentle shaking for 15 min. The sterilized materials were washed 4-5 times with sterile distilled water immediately to remove all traces of HgCl₂. The basic culture medium was MS supplemented with various concentrations and combinations of IAA, 2, 4-D NAA, BAP and Kn and. The media were adjusted to pH 5.8 and subsequently were jelled with 0.8% agar and 3% sugar and autoclaved for 20 min at 121°C and 1.1 kg cm². Explants were inoculated longitudinally on to media containing culture bottle in a laminar flow cabinet. Cultures were incubated at 26+1°C under the warm in fluorescence light intensity varied from 2000-3000 lux. The photo period was maintained 16 h light and 8 h dark. Calluses were aseptically weighed and regenerated shoot lengths were measured.

Source of Information: Journal of Plant Sciences, 2(4): 398-406, 2007, ISSN 1816-4951.

Article Link (Online Journal): DOI: 10.3923/jps.2007.398.406; URL: http://scialert.net/abstract/?doi=jps.2007.398.406

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Regenerated plants were then cultured on to a rooting medium containing different concentration and combination of growth regulators of which only 0.4 mg L^-1 IAA showed best performance for root induction and profuse root proliferation occurred within 7-10 days from culture. Plant lets with rooting were removed from the culture vessels and then washed with running distilled water or running tap water and then transferred to wet fertile loamy soil contained plastic pots for hardening. Within one months or before the plant lets showed best performance for acclimatization.

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