Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Alteration of Cellular Pigments of Papaya Leaves Infected with Seven Symptomatic Isolates of Prsv-p

H. Rahman
Department of Plant Pathology, Patuakhali Science and Technology University, Dumki, Patuakhali, Bangladesh.

M. M. Alam
Department of Genetics and Plant Breeding, Patuakhali Science and Technology University, Dumki, Patuakhali-8602, Bangladesh.

S. B. Bhyan
Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.

A. M. Akanda
Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur, Bangladesh.

Abstract/ Executive Summary:

An experiment was conducted at the Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU) experimental field, Gazipur, Bangladesh in 2003 to determine the diverse change in the amount of cellular pigment contents of papaya leaves infected with different biological variants of Papaya ring spot virus-Papaya strain (PRSV-P). Among the naturally prevailing PRSV-P strains in Bangladesh. Seven different and distinctly defined ailments of symptoms, namely Mild mosaic, mosaic, fern leaf, severe mosaic, vein clearing, leaf distortion and chlorotic leaf spot were selected to conduct the tests of this experimentation. Different partition of cellular pigments (Chlorophyll-a, Chlorophyll-b and ß-carotene) were measured to quantify the alteration of cellular components. For every partition of pigments tested, the highest alteration was found in leaf bearing leaf distortion symptom. In most of the cases the lowest shift in pigment content was determined with the leaf showing Mild Mosaic symptom followed by the mosaic, severe mosaic, vein clearing, chlorotic leaf spot, fern leaf and leaf distortion symptom respectively. The variability in all the partition of pigments contents alteration was found to be consistently correlated with 7 symptomatic variants of PRSV-P.

Keywords: Papaya, PRSV-P, Symptomatic Isolates, Cellular pigments, Partition,

Location: Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU) Farm, Gazipur, Bangladesh.

Start Date: 00-00-2003

End Date: 00-00-2003

Program Area: Pest Management

Commodity: Diseases

Objectives:

To elucidate the change in the amount of cellular pigment contents of papaya leaves infected with different biological variants

Methodology:

Papaya variety Shahi was grown at Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU) experimental field, Gazipur, Bangladesh in 2003. Papaya plants infected with Papaya ring spot virus-Papaya strain showing seven different types of symptoms named by the seven symptomatic isolates were selected and tagged. Leaf samples were collected from the healthy and infected plants from the similar position and similar age showing distinct symptoms to maintain the uniformity of the sample. Three samples were collected at three different growth stages of plants (Early = before fruit setting, mid = fruit setting to first ripening and late = first ripening onward) infected with each symptomatic isolates. The healthy and infected plants were frequently checked for PRSV-P infection using mechanical sap inoculation and DAS-ELISA methods, respectively. A double beam spectrophotometer (Model No. 1200-20, Hitachi) was used to measure the absorbance value/Optical Density (OD) when it was required. All pigment partition was measured from the both healthy and PRSV-P infected leaf samples. Chlorophyll content was determined by extraction with 80% acetone method. Statistical analysis was done using DMRT.

Source of Information: Journal of Plant Sciences, 3(1): 69-76, 2008, ISSN 1816-4951.

Article Link (Online Journal): DOI: 10.3923/jps.2008.69.76; URL: http://scialert.net/abstract/?doi=jps.2008.69.76

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The results of the present study demonstrated that seven different symptomatic isolates of PRSV-P infecting papaya in Bangladesh might be the mutant strains of PRSV-P which distinctly differed in inducing the alternations of chlorophyll in their respective infected papaya leaves.

Knowledge Management: Journal