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Isolation, Identification and Determination of Antibiotic Susceptibility of Vibrio Parahaemolyticus from Shrimp at Khulna Region of Bangladesh

Abdul Wahab Khan
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh.

S. J. Hossain
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh.

Sarder Nasir Uddin
Assistant Professor
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh.

Abstract/ Executive Summary:

To investigate the antibiotic susceptibility, Vibrio parahaemolyticus were isolated and identified from the sample of shrimps collected from different shrimp processing plants located at Rupsha, Khulna. Among the 27 samples, 10 strains were identified as Vibrio parahaemolyticus. Susceptibility of these Vibrio parahaemolyticus was assayed by disc diffusion method against 11 antibiotics. All these strains were highly sensitive to Tetracycline, Norfloxacin, Gentamicin, Riphampicin and Neomycin, but were resistant to Erythromycin, Penicillin, Ampicillin and Kanamycin. Vibrio parahaemolyticus showed both resistant and susceptible character against Cephalexin and Streptomycin. 7 strains were resistant and 3 were sensitive to Cephalexin on the other hand 8 were resistant and 2 were sensitive to Streptomycin, among the 10 Vibrio parahaemolyticus strains. The antibiotics named Tetracycline, Norfloxacin, Gentamicin, Riphampicin and Neomycin should be the choice to control diseases due to Vibrio parahaemolyticus as well as enteric bacteria.

Keywords: Antibiotic, Shrimp, Therapy

Location: Khulna district

Start Date:

End Date:

Program Area: Pest Management

Commodity: Shrimp

Objectives:

(a) To Isolate and identification of Vibrio parahaemolyticus from shrimp

(b) To study of antibiotic susceptibility of Vibrio parahaemolyticus species.

Methodology:

Shrimp samples were collected from different shrimp processing plant in Khulna district. For the collection of samples some clean, dry and sterile polythene bags were taken to the sampling site. Sufficient amount of fish was collected from each site; kept in the polythene bag and tagged. Measures were taken to avoid contamination as far as possible. Samples were processed immediately in the laboratory. Samples were kept refrigerated (4°C) until culture. Aseptically weigh a 25 g shrimp into a sterile stomacher bag. Cut large sample into smaller pieces before stomaching. After stomach, add additional Alkaline Peptone Water (APW) to bring the total amount added to 225 mL (10^-1 dilution). Now APW was incubated for 24 h at 37°C. Plating on Selective Agar Media, Maintenance and Preservation of the Selected Strains, Identification of Vibrio parahaemolyticus, Identification of Vibrio parahaemolyticus, Oxidase Test, Lysine Decarboxylase Test, Arginine Dihydrolase Test, Salt Tolerance Test, Voges-proskauer Test, Carbohydrate Fermentation Test, Gelatin Hydrolysis Test, Urease Test, Antibiotic Susceptibility of Isolated Vibrio parahaemolyticus, Preparation of Müeller-Hinton Agar, Turbidity Standard for Inoculum Preparation, Preparation of Inoculum for Performing the Disc Diffusion Test, Inoculation of Test Plates, Application of Discs to Inoculated Agar Plates were discuss in this study. After 16 to 18 h of incubation, each plate was examined. The diameters of the zones of complete inhibition (as judged by the unaided eye) were measured, including the diameter of the disc. Zones were measured to the nearest whole millimeter, using a ruler, which was held on the back of the inverted petri plate. The zone margin was taken as the area showing no obvious, visible growth that can be detected with the unaided eye. Faint growth of tiny colonies, which can be detected only with a magnifying lens at the edge of the zone of inhibited growth, was ignored. However, discrete colonies growing within a clear zone of inhibition were subcultured, re-identified and retested.

Source of Information: Research Journal of Microbiology, 2: 216-227, 2007, ISSN 1816 - 4919

Article Link (Online Journal): DOI: 10.3923/jm.2007.216.227; URL: http://scialert.net/abstract/?doi=jm.2007.216.227

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Vibrio parahaemolyticus isolated in this study showed good susceptibility to most of the antimicrobial agents that are commercially available for the treatment of causing disease. From this study, there was a significantly larger number of resistant isolates for Erythromycin, Penicillin, Ampicillin and Kanamycin suggesting that antibiotic use in these shrimp’s farms may be related to increased resistance.

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