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Fertility of Cow in Using Locally Produced Chilled and Imported Frozen Semen

A. K. Das
Department of Animal Breeding and Genetics, BAU, Mymensingh, Bangladesh.

M. Y. Ali
Livestock Development Program, Proshika, Dhaka, Bangladesh.

M. A. Islam
Poultry Research Division, BLRI, Savar, Dhaka, Bangladesh.

A. K. Hira
Livestock Development Program, Proshika, Dhaka, Bangladesh.

S. Z. Ali
Department of Animal Breeding and Genetics, BAU, Mymensingh, Bangladesh.

Abstract/ Executive Summary:

The experiment was carried out at Central Cattle Breeding Station and Dairy farm, Savar, Dhaka, and 3 sub-station and 9 points of Chandpur District in Bangladesh to evaluate the quality and fertilizing capacity of locally produced chilled and imported frozen semen. Motility, sperm concentration and mass activity of semen from different experimental bulls were almost similar. Quality of imported frozen semen was better than that of locally produced chilled semen in respect of motility, motile sperm/ Insemination dose and spermatozoa with normal head. Motility and pH value of semen decreased significantly for transportation and prolongation of preservation duration. Average conception rate of imported frozen semen (57.33) was found to be higher than locally produced chilled semen (45.33). But it was similar between imported frozen (57.33) and average of 1^st & 2^nd day preserved semen (57%).

Keywords: Cow, Chilled semen, frozen semen, Semen quality, Microscope, Transportation, Preservation, Ice, Fertility

Location: Cattle Breeding Station and Dairy farm, Savar, Dhaka

Start Date: 00-07-1998

End Date: 00-06-1999

Program Area: Variety and Species

Commodity: Cow

Objectives:

To compare the fertility of cow in inseminating by imported frozen and locally produced chilled semen.

Methodology:

The experiment was conducted between July, 1998 and June 1999 at Central Cattle Breeding Station, (CCBS) and Dairy Farm, Savar, Dhaka, 3 sub-centers and 9 points of Chandpur District, Bangladesh. Semen from different crossbred bulls was collected using artificial vagina once in a week at CCBS, and twice in a week at Chandpur district AI center, and diluted with egg yolk citrate extender remaining 20 x 10⁶ progressively motile spermatozoa per million. Individual insemination doses were transferred in glass vials covered by cork and preserved at 4-8°c until used. Volume, colour, mass activity of semen, sperm concentration and motility of fresh ejaculated semen from locally bred experimental bulls were recorded. The mass activity of semen was evaluated in a drop of fresh undiluted semen placing on a warm slide without cover slip at low magnification (400X). The mass activity was graded as (+ 1 to+4,+ 1 = no activity ,+ 2 = slow wave motion, +3 = rapid wave motion with formation of eddis at the end of waves, + 4 = eddies). Concentration of spermatozoa (million/ml) in fresh semen was determined by Kagras scale. A small drop of diluted semen was placed on a microscopic slide, covered with a cover slip and examined at magnification of 400 X under light microscope. Only sperm moving in a straight forward direction were included in the motility count. Both mass activities were examined at body temperature of 37°C. pH: Semen pH was measured using indicator paper strips (nitrazene paper). It was done by staining the smear of semen with William's method. Five hundred cells were counted under microscope of oil emersion objective (X100). Five hundred normal sperms from individual smell's were recorded. Big, narrow, pear shaped, short, broad and abnormal contour head or any other deformities of sperm were treated as abnormal sperms. Fifteen villages under 3 sub-centres having 50 cows in each were selected. After detection the sign of estrus, the cows were inseminated using locally produced chilled or imported frozen semen by the inseminators. To test the accurate pregnancy, milk samples were collected from the experimental cows and determined the progesterone level immediately before palpation. A decline level of progesterone at about days 17 - 21 after insemination indicated the non pregnancy, while a stable or rising level indicated pregnancy. In addition 60-90 days after insemination, pregnancy was confirmed by per rectal palpation of the genital tract.Data were analyzed using MSTAT computer Package program. The quality of locally produced and imported frozen semen were tested using F-test. Data for transportation and preservation effect on quality of locally chilled semen, and conception rate between locally chilled and imported frozen semen were tested using t- test.

Source of Information: Pakistan Journal of Biological Sciences 5(2): 215-217, 2002

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Non significant differences were found for the quality of semen from locally produced chilled and imported frozen semen (P>0.05). However motility, motile sperm/insemination dose, and spermatozoa with normal head tended to increase in imported frozen semen compared to locally chilled semen. Motility of spermatozoa and the pH value of semen were significantly (P<0.05) decreased for transportation and prolongation of preservation time. Conception rate of imported frozen semen was significantly higher (57.33%) than that of locally produced chilled semen (45.33%) (P<0.05). There was no significant difference between 1^st and 2^nd groups of chilled semen (P>0.05). The conception were significantly higher in cows inseminated by 1^st and 2^nd groups than those of inseminated by 3^rd groups of chilled semen. Nevertheless the difference in the proportion of conception rate between imported frozen and two groups (1^st and 2^nd) of locally chilled semen was not significant (P>0.05).

Knowledge Management: Journal