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Establishment and Growth of Potato Micro-cuttings in Sand Trays

Md. Jahangir Hossain
Research Wing, Bangladesh Agricultural Research Institute, Joydebpur, Bangladesh.

Mosammat Shamsun Nahar
Plant Pathology Section, Horticulture Re-search Center, Bangladesh Agricultural Research Institute, Joydebpur, Bangladesh.

Abstract/ Executive Summary:

Two separate experiments were conducted to evaluate the success of the establishment and growth of micro-cuttings of potato (5 - 6 cm tall) in sand trays [38 cm (L) × 28 cm (W) × 7.5 cm (H) plastic trays] under controlled environment (22?C ± 2?C, 60 - 75 μmosm^-1·s^-1 light energy for 16 h daily). In the first experiment, micro-cuttings of potato cv. Diamant were planted at six populations (500, 600, 700, 800, 900 and 1000 cuttings per tray) in treated sand (sun dry, 1% formaldehyde, 0.2% Dithane M-45 and control). The mortality percentage of micro-cuttings was nil for sun dry sand while formaldehyde and dithane M-45 treated sand had 1% - 4% against 15% in the control with the highest population density. Mortality of micro-cuttings in formaldehyde and dithane M-45 treated sand trays were found not to be related to pathogenic organism rather toxic effect of these two chemicals. Micro-cuttings in Sun dry and control treatments showed better growth performance than these in chemically treated sand trays. In the second experiment, urea @ 1, 2 and 3 g per tray was applied as solid form after 15 days of planting the micro-cuttings and as liquid form @ 0.5, 1 and 2% solution sprayed in the micro-cuttings repeatedly after 15, 30, 45 and 60 days of planting. The micro-cuttings which received urea as solid state died within 2 - 3 days and 2% urea solution was also detrimental. Urea solution @ 0.5% found to be very effective for vegetative growth of micro-cuttings in sand trays. The control was also good for vegetative growth but at a slower rate.

Keywords: Sand tray, Sun dry, Fungicides, Urea fertilizer, Plant population, Growth, Potato micro-cuttings

Location: Bangladesh Agricultural Research Institute, Joydebpur, Bangladesh.

Start Date: 00-00-2006

End Date: 00-00-2007

Program Area: Variety and Species

Commodity: Potato

Objectives:

To standardize the methods for better establishment and growth of micro-cuttings of potato in sand trays under controlled environment.

Methodology:

Micro-cuttings of three Dutch potato cultivars Cardinal, Diamant and Multa were used in two separate experiments to standardize the methods for better establishment and growth in sand trays under controlled environment during 2006 and 2007. In the experiments in vitro micro-plants (4 - 6 cm tall) of 30 days old were first transplanted in plastic trays of 38 cm (L) × 28 cm (W) × 7.5 cm (H) contained 0.013 mm size sand particle. The transplanted micro-plants were nursed under controlled environment (22?C ± 2?C, 60 - 75 µmosm^-1·s^-1 light energy for 16h daily). After 25 days of planting, top shoots (1.5 - 2.0 cm) were cut and planted in sand trays of above. In the first experiment, only one cv. Diamant was used. Sand were treated with four different (sun dry, 1% for- maldehyde solution, 0.2% dithane M-45 and untreated control) ways. Sand at 5 cm heap were sun dried for two days while sand were treated with formaldehyde and dithane M-45 before 7 and 3 days respectively of planting the micro-cuttings. Micro-cuttings population (500, 600, 700, 800, 900 and 1000 per tray which was equivalent to 4750, 5700, 6650, 7600, 8550 and 9500 m^-2) was maintained. The sand trays were kept under the controlled environment as stated previously. The experiment was laid in a randomized complete block design with four replications. The experiments were set on 15 April in both the years, 2006 and 2007. In the second experiment, three Dutch potato cultivars Cardinal, Diamant and Multa were used. Urea @ 1, 2 and 3 g per tray in solid form and 0.5, 1 and 2% urea solution were applied to standing crop of micro-cuttings. No urea was used in the control trays. Urea in solid form was applied 15 days after planting the micro-cuttings in sand trays, while spraying of urea solution started after 15 days of planting the micro-cuttings and sprayed for four times at 15-days interval. The experiment was set in a randomized complete block design with three replications. The experiments were set on June 20 in both the years, 2006 and 2007. As the recorded data on different parameters of both the experiments conducted in two years, 2006 and 2007 did not vary statistically, thus the mean data were used to analyze the experiment.

Source of Information: American Journal of Plant Sciences, 2012, 3, 1219-1224

Article Link (Online Journal): http://dx.doi.org/10.4236/ajps.2012.39147

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It may be concluded that sundry sand is the most effective soil substrate for development and growth of micro-plants under controlled environment and spraying of 0.5% urea solution on foliage can safely be done to enhance the growth of micro-cuttings.

Knowledge Management: Journal