Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Sterilization and Male Ratio Optimization of Peach Fruit Fly, Bactrocera Zonata (saunders) for Field Application in Sterile Insect Technique

M.R. Islam
M.Sc. Student
Department of Zoology, Dhaka College, Dhaka, Bangladesh

M. Momen
Senior Scientific Officer
Insect Biotechnology Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Ganakbari, Savar, Dhaka 1349, Bangladesh.

M.A. Hossain
Principal Scientific Officer
Insect Biotechnology Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Ganakbari, Savar, Dhaka 1349, Bangladesh.

S.A. Khan
Chief Scientific Officer
Insect Biotechnology Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Ganakbari, Savar, Dhaka 1349, Bangladesh.

N.P. Khanom
Professor
Department of Zoology, Dhaka College, Dhaka, Bangladesh

Abstract/ Executive Summary:

The experiment was conducted for the sterilization and sterile male ratio optimization of peach fruit fly, Bactrocera zonata (Saunders) for field application in Sterile Insect Technique (SIT). To determine the male sterility dose 6 days old pupae of B. zonata were irradiated with doses of 10, 30, 40, 50 and 60 Gy. It was noted that percent of adult emergence and egg hatch decreased significantly with increasing radiation dose (p<0.01). The sterility percentage by a percent of egg hatch of B. zonata increased as the dose increased. Cent percent male sterility was achieved at 60 Gy. Fixed numbers (150 females) of virgin females were allowed to mate with normal and sterile (60 Gy) males at 1:1, 1:2, 1:5 and 1:9 ratios. Comparing with control group, a sharp decrease of pupal recovery was observed when females were allowed to mate with different ratios of sterile males. Minimum pupal recovery (42.10 fold less) was obtained at 1:9 ratio. Percent of normal adult emergence was decreased significanly (p<0.01) as the sterile male ratios were increased from 1:2 to 1:9. Deformed adult emergence, partial adult emergence and un-emerged percentage of peach fruit flies were increased significantly as the ratios of sterile males were increased.

Keywords: Sterility dose, Irradiation, Ratio optimization, Bactrocera zonata

Location: laboratory, Insect Biotechnology Division, Institute of Food and Radiation Biology (IFRB), Atomic Energy Research Establishment (AERE), Savar, Dhaka.

Start Date:

End Date:

Program Area: Pest Management

Non-commodity: Insects

Objectives:

The study was conducted to study the sterilization and male ratios optimization of sterile and normal males for possible field applications of SIT.

Methodology:

Stock culture: Laboratory cultures of B. zonata were maintained in the fruit fly laboratory, Insect Biotechnology Division, Institute of Food and Radiation Biology (IFRB), Atomic Energy Research Establishment (AERE), Savar, Dhaka. Larvae were maintained on artificial diet. Adult peach flies were stocked in a wooden frame cage (60×50×45 cm) covered with nylon net. The culture was usually supplied with an artificial diet (sugar:yeast extract:casein/1:2:1) and water soaked cotton. In general, 1500-2000 adult fruit flies were maintained in a stock cage. Temperature and relative humidity of the rearing room were maintained at 28±2^oC and 70-80% respectively. Pupae collection and irradiation: The larvae of B. zonata in the artificial larval diets were kept in plastic bowls with saw dusts at the bottom for pupation. The saw dusts were sieved after pupation for the collection of dust free pupae. Same aged (6 days old) 100 pupae were transferred into Perti dishes and irradiated in a Co⁶⁰ gamma irradiator of IFRB, AERE, Savar, Dhaka. Radiation doses ranging from 10 to 60 Gy were applied with a dose rate of 10 Gy/minute. Each dose group had 4 replications and was repeated 5 times. One batch of control was also maintained with equal number of replications. Adult emergence percentage, sex ratio, fecundity data were collected and noted. Sterility dose determination: Fifty male flies were collected from each of 10, 30, 40, 50 and 60 Gy treated 6 day old pupae and fifty females were also isolated from the control batch and allowed to mate with individual dose treated males in laboratory rearing cages. This experiment had four replications. After two days of mating, eggs were collected in egging device inside rubbed with banana paste. Collected eggs were poured on a tissue paper and placed on larval diet. Hatching data were collected after 48 hours. Male ratio optimization: The six days old pupae of B. zonata were exposed to 60 Gy gamma irradiation. The adults emerged from the pupae were sexed and males and females were sorted out. The sterile and normal males were allowed to mate with 150 virgin females at 1:1 (75 normal and 75 sterile males), 1:2 (50 normal and 100 sterile males), 1:5 (25 normal and 125 sterile males) and 1:9 (15 normal and 135 sterile males) ratios in separate adult cages. Oviposition media (piece of banana) was placed in each of the cage after 2^nd days of mating. The piece of banana was removed after 24 hrs from the adult cage and placed it on artificial diet. The total numbers of pupae and adult emergence were counted and recorded. One hundred and fifty laboratory reared males and same number of females were used as control. The raw data were analyzed by statistical software (Minitab version 13.1, Microsoft Excel). For Analysis of Variance (ANOVA) of percentage data, percentage values were transformed into Arcsine values.

Source of Information: Int. J. Expt. Agric. 9(1):1-5(March 2019)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Data generated from this experiment on sterility dose, pupal duration and on the ratios of irradiated males of Bactrocera zonata clearly revealed that 60 Gy could be used as a sterilizing dose for this species and a 9 fold ratio of irradiated male fly to unirradiated one could produce significant suppression of peach fruit fly. However, further works with larger volume will be required to generate a data more suitable for field applications of peach fruit fly SIT.

Knowledge Management: Journal