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Effect of Sunflower Oil Supplementation in Feed on Body Weight and Hematobiochemical Parameters in Mice

Basak A
Department of Physiology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

Banu LA
Department of Physiology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

Ahmad N
Department of Physiology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

Rafiq K
Department of Pharmacology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

Abstract/ Executive Summary:

Sunflower oil contain high concentrations of essential polyunsaturated fat with antioxidant vitamin E. The study was carried out on Swiss Albino healthy mice to evaluate the effects of additional supplementation of sunflower oil in feed on body weight, hematological parameters (total erythrocyte count and hemoglobin concentration) and biochemical parameters (total serum cholesterol, triglycerides, high density lipoproteins, low density lipoprotein and serum creatine). A total of 20, three-weeks old mice were randomly divided into two equal groups ( n=10) as A and B. Group A was considered as control (standard poultry pellet) and group B was supplemented with sunflower oil (5 mL/kg feed) respectively in addition to standard poultry pellet for 50 days. At the end of experimental trial, the mice were sacrificed for analysis of hemato-biochemical parameters. Body weight was significantly (P<0.05) higher in sunflower oil supplemented group compared to control. The total erythrocyte count and hemoglobin concentration were increased significantly (P<0.05) in group B compared to control group A. The total serum cholesterol, HDL and LDL values increased significantly (P<0.05) whereas the triglycerides concentration decreased significantly (P<0.05) in sunflower oil treated group compared to control group. On the other hand serum creatinine value showed non-significant trend to increase (P<0.2). These results suggest that sunflower oil may have beneficial effects to maintain healthy life style. Although further studies are needed to find out the molecular mechanism and its toxicological aspect if any.

Keywords: Sunflower oil, Dietary supplement, Hematobiochemical parameters, Mice, HDL, LDL, Physiology

Location: Department of Physiology, Bangladesh Agricultural University (BAU), Mymensingh

Start Date: 00-01-2016

End Date: 00-05-2016

Program Area: Animal Health and Management

Commodity: Sunflower

Objectives:

To estimate the effects of sunflower oil on body weight, haematological parameters (total erythrocyte countand hemoglobin concentration) and lipid profiles i.e. triglyceride, total cholesterol, high density lipoprotein (HDL) and low density lipoprotein (LDL) and serum creatinine in mice.

Methodology:

The experiment was conducted in the Department of Physiology, Bangladesh Agricultural University (BAU), Mymensingh over a period of January to May 2016, to study the effects of sunflower oil on hemato- biochemical parameters in mice. Experimental mice: The mice were purchased from Department of Pharmacy, Jahangirnagar University, Savar, Dhaka and adapted for 10 days in order to acclimatize them with the new environment. All mice were housed in a compartmentalized square wooden cages wrapped with wire mesh. The cages were kept in well ventilated room at 28± 2°C and a relative humidity of 70-80% with natural day and light. Experimental design: A total of 20, the male Swiss Albino mice having three weeks of age were used in this experiment. The mice were randomly divided into two equal groups (n=10 in each group). Group A was considered Control and fed with standard poultry pellet only and Group B was fed with sunflower oil (5 mL/kg feed) in poultry pellet. All groups were supplied with standard poultry pellet and fresh drinking water adlibitum. The several parameters measured that includes body weight, hematological and biochemical tests. The mice were sacrificed to collect blood sample for hematological study and biochemical parameters such as the lipid profile i.e. triglyceride, total cholesterol, high density lipoprotein (HDL) and low density lipoprotein (LDL) and serum creatinine. Blood was collected directly from the heart of the mice using general anaesthesia. Management practices: The cages were kept on a well-ventilated room. In order to prevent spoilage feeds were kept in poly packed sac. The feed and fresh drinking water were supplied daily to the mice. Mice cages were cleaned regularly and proper hygienic and sanitary measures were also allowed during the experimental period. Measurement of body weight: The body weight of each mice was measured with the help of electric balance at 7 days interval up to the end of the experiment. Collection of blood: The mice were placed inside the air tight container one by one containing cotton soaked with chloroform. After anesthetized they were taken out from the container for blood collection. The abdominal cavity and thoracic cavity were opened surgically and blood was collected directly from the heart with the sterile syringe and needle. About 1 mL of blood from the syringe was taken in the test tube containing anticoagulant (3.8% Sodium citrate solution) for hematological studies. The remaining amount of blood of syringe was used for collection of serum. Preparation of serum: Two mL of blood was collected in the sterile glass test tube. The blood containing tubes were placed in slanting position at room temperature for 6 hours. The tubes were then incubated overnight in the refrigerator (4°C). The serum samples were separated and centrifuged at 1500 at rpm for 15 minutes to get rid of unwanted blood cells if necessary. Serum samples were stored at -20°C for biochemical analysis. Biochemical analysis: The serum biochemical parameters like the lipid profile i.e. triglyceride, total cholesterol, high density lipoprotein (HDL) and low density lipoprotein (LDL), serum creatinine were performed using auto analyzer in the Central Laboratory of Bangladesh Agricultural University, Mymensingh. Statistical analysis: The data were calculated and analyzed using statistical SPSS program. The study was expressed as Mean ±SE. The data were analysed using “student t” test and P<0.05 or less was considered as statistically significant. (SPSS., Chicago, IL, U/L).

Source of Information: Progressive Agriculture 28 (1): 36-41, 2017

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present study suggested that sunflower oil supplementation have significant beneficial effect on hemato-biochemical parameters in mice. For the establishment of beneficiary effect of such oil, further study is necessary to observe any adverse effect in relation to histopathology and biochemistry before making any definite conclusion regarding the beneficial effect in mice as well as human being

Knowledge Management: Journal