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Effect of Polyethylene Glycol Induced Water Stress on Germination and Seedling Growth of Wheat (triticum Aestivum)

M.S. Rana
Department of Crop Physiology and Ecology Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.

M.A. Hasan
Department of Crop Physiology and Ecology Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.

M.M. Bahadur
Department of Crop Physiology and Ecology Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.

M.R. Islam
Department of Crop Physiology and Ecology Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.

Abstract/ Executive Summary:

The performance of twenty wheat genotypes under Polyethylene Glycol (PEG) induced water stress during germination and early seedling growth stages were tested under three levels of water potential i) Control (Tap water), ii) -2 bars and iii) - 4 bar at the Crop Physiology and Ecology Laboratory of Hajee Mohammad Danesh Science and Technology University, Dinajpur during September 2014 to October 2014. Rate of germination and vigor index of all wheat genotypes were delayed with the increment of water stress induced by PEG. Shoot and root lengths and seedling dry weight of 10 days old seedlings were found to be reduced due to the increment of water stress. However, the degree of reduction of these parameters with the increment of water stress was not similar for all wheat genotypes. Stress tolerance index (STI) based on seedling dry weight indicated a wide difference in stress tolerance among the wheat genotypes. At moderate water deficit stress, BARI Gom 25, E 34, E 28 and BAW 1170 showed more stress tolerance and the wheat genotypes- Sourav, E 23 and BAW 1140 showed greater stress sensitivity than the other wheat genotypes. At higher water deficit stress, BARI Gom 25, BARI Gom 28, E 28 and BAW 1170 showed more stress tolerance and the wheat genotypes- Satabdi, Sourav, BARI Gom 26, E 23, E 38, E 24, BAW 1163, BAW 1140 and BAW 1151 showed greater stress sensitivity than the others. Considering both moderate and high water deficit stress, BARI Gom 25, E 28 and BAW 1170 were found as tolerant and Sourav, E 23 and BAW 1140 were found as water deficit stress sensitive wheat genotypes.

Keywords: Stress tolerance index, Germination, Water Stress, Seedling Growth, Wheat

Location: Crop Physiology and Ecology Laboratory, Hajee Mohammad Danesh Science and Technology University, Dinajpur

Start Date: 00-09-2014

End Date: 00-11-2014

Program Area: Crop-Soil-Water Management

Commodity: Wheat

Objectives:

To evaluate germination characters and seedling growth of wheat as screening criteria against drought stress.

Methodology:

Location and duration The experiment was conducted at Crop Physiology and Ecology Laboratory, Hajee Mohammad Danesh Science and Technology University, Dinajpur during September 2014 to October 2014. Experimental design and treatments- The experiment was carried out in completely randomized design (factorial) with three replications. One factor consisted of three levels of water potential- Control (Tap water), moderate stress (-2 bars induced PEG 6000) and higher stress (-4 bars induced by PEG 6000) and the other factor consisted of twenty wheat genotypes including advance lines and popular varieties. Collection and placement of seed- Seeds of twenty wheat genotypes collected from Wheat Research Centre of BARI, Dinajpur, Bangladesh were surface sterilized by dipping the seeds in 0.1% mercuric chloride solution for 2 minutes and rinsed thoroughly with sterilized water. Required amount of PEG 6000 was dissolved in tap water as described by (Michel, 1983) to develop -2 bars and -4 bar water potential. Thirty seeds of each genotype were placed for germination in 11 cm petridish on sand bed irrigated with respective treatments. The petridishes were irrigated daily with required amount (5 ml) of respective solution. Each treatment was replicated thrice. Seedlings were allowed to grow up to 7 days after placement for germination. Data recorded and analysis- Germination was counted at 24-hour interval and continued upto 7^th day (168 hrs). A seed was considered germinated as plumule and radicle came out and were >2 mm long. The rate of germination was calculated using the formula as described by Krishnasamy and Seshu (1990) and co-efficient of germination and vigor index were calculated using the formulae as described by Copeland (1976). At 7^th day after placement for germination, all other seedlings were removed from each petridish keeping 10 healthy seedlings and allowed to grow up to 10 days by adding required amount (5 ml) of respective solution daily. At 10 days after placement for germination, five seedlings from each petridish were sampled. Shoot and root length of individual seedling were recorded manually with scale. Then the seedlings were dried separately at 70⁰C for 72 h in an electric oven (Model- E28 # 03-54639, Binder, Germany) and weight were recorded with an electrical balance (Model- EK- 300 i). The mean length (cm) and dry weight were recorded for each treatment combination. Stress tolerance index was calculated as Goudarzi and Pakniyat (2008). The data were analyzed by partitioning the total variance with the help of computer using MSTAT program.

Source of Information: The Agriculturists 15(1): 81-91 (2017)

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Based on seedling dry weight, under both moderate and high water deficit stress BARI Gom 25, E 28 and BAW 1170 were found as tolerant and Sourav, E 23 and BAW 1140 were found as water deficit stress sensitive wheat genotypes during germination.

Knowledge Management: Journal