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Research Detail

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Md. Shakil Islam
Department of Dairy Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Rezwanul Habib
Department of Dairy Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Mohammad Ashiqul Islam
Department of Dairy Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Harun-ur-Rashid
Department of Dairy Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

This experiment was conducted to study the effect of protein rich ingredients supplementation into concentrate diet on body growth and some biochemical blood parameters of crossbred heifers. Six heifers aged above two years were divided into two equal groups: (a) control (farm feeding practices) and (b) protein supplemented. Soybean meal and mustard oil cake were added in concentrate mixture as protein supplement in supplementary group. The heifers of both dietary groups had access to required amount of para and german grass as basal roughage. Growth measurement parameters were studied at fortnightly; blood collection and its analysis for various blood metabolites were assayed at thirty days intervals. It was noticed that heifer dry matter intake was significantly (P=0.001) affected by the diet and found higher in supplemented group than control group. About 16% higher (P=0.149) body weight gain was found in supplemented group compared to control group. Protein supplemented heifer showed 0.12, 0.74 and 0.26 cm higher gains for wither height (P=0.01), body length (P=0.002) and heart girth (P=0.016), respectively. The blood biochemical profile revealed that serum urea and blood urea nitrogen concentrations were higher (P=0.015) in protein supplemented group compared to that of the control group. In conclusion, supplementation of protein rich ingredients in heifer diets might increase the growth measurements and blood serum protein status which would be more advantageous for the development of dairy herd replacement stock.
 

  Soybean meal, Mustard oil cake, Growth, Blood serum, Heifers
  Dairy Farm, Department of Dairy Science, Bangladesh Agricultural University, Mymensingh-2202
  
  
  Quality and Nutrition
  Cattle

The objective of this research was to observe the effect of feeding protein rich ingredients along with concentrate mixture on the growth and nutritional status of the dairy heifers

Animal procedures were conducted at the Dairy Farm, Department of Dairy Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. Six (06) crossbred heifers (50% Sahiwal, ShX; and 50% Holstein Friesian, HFX) whose aged above 2 years were divided into two groups like control and supplemented; and the average live weight of each group was 150±5 kg. The study was conducted for a period of 100 days of which first 10 days were for diet adjustment. Each heifer was kept in a face-out stanchion barn with individual manger and water trough. The basal diet consists of para grass (Brachiaria mutica) and german grass (Echinochloa crus-galli) which were supplied at 50:50 (18 kg/hd/d). Mixed concentrate (1.9kg/hd/d) was offered to the heifers twice daily. Concentrate ingredients in control diet included rice polish, wheat bran, DCP and common salt. In addition to these, mustard oil cake and soybean meal were supplied to the supplemented group. Dry matter was offered @ 3.5% of the live weight. Concentrate was fed prior to the green grass at 08:00 am and at 13:00 pm. Proximate composition of each ingredients were determined as described by AOAC (2000). Estimation of DMI and growth measurement  DM intake (kg) calculated from supplied feed and daily leftover. Body weight, body length, heart-girth and wither height of the experimental heifers were measured through weighing balance, measuring tape and wooden scale, respectively. Data for these variables were recorded on each heifer at fifteen days interval. Collection of blood and serum analysis  About 10 mL blood was collected at 30 days intervals from jugular vein of each experimental heifer and kept in a tube (without anticoagulant). Then collected blood was allowed to clot at room temperature for a period of time (40-45 minutes). Thereafter, centrifugation was done using centrifuge (EBA 21 Hettich Zentrifugen, Germany) at 3000 rpm for 15 min. Following these, serum was harvested and kept in kitchen freezer until assayed. Glucose level in blood serum was measured through glucose oxidase and peroxidase method using enzymatic quantitative colorimetric kit (Vitro Scient, Egypt). Quantitative colorimetric kits albumin (Vivia-Biotech., Spain) and urea-LQ (Chemelex, Spain) were used to measure blood serum albumin and urea concentrations, respectively. The factor 2.14 was used to obtain the concentration of blood urea. URIT-810 chemistry analyser (URIT Medical Electronic Company Limited, China) was used as at 550, 630 and 340 nm wavelengths to measure the serum glucose, albumin and urea, respectively. Repeated Measures Analysis of Variance (RMANOVA) in Completely Randomized Design was executed to scrutinize the impacts of protein rich ingredients feeding to the dairy heifers on DMI, growth performance and blood serum nutrients. In this analysis, experimental heifer’s initial body weight was not thought as a covariate because of non-significant influence on the dependent variables. Again, period of time was treated in the within-subjects factor to measure the parameters recurrently on the experimental units; whereas, dietary groups (independent variable) were subjected in between-subjects factor.
 
 

  J Bangladesh Agril Univ 17(1): 45–49, 2019
  https://doi.org/10.3329/jbau.v17i1.40662
Funding Source:
  

Supplementation of protein rich ingredients (soybean meal and mustard oil cake) in the concentrate mixture of heifers influences dry matter intake and nutritional status. During pre-pubertal period this nutritional status of heifers indicates the impact of such feeding programs for initiating heifer development and replacement. For improving heifer ration quality this findings might have implication in the tropical countries where cattle are often fed rations deficient in protein. It could be concluded that feeding protein rich ingredients with concentrate mixture would effectively improve the growth and nutritional status of the dairy heifers.

  Journal
  


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