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Effect of Indole Butyric Acid on Raising Plants from Stem Cuttings of Tea (camellia Sinensis L.) in the Nursery

Mohammed Enamul Hoque
Bangladesh Tea Board, Regional Office, Soalok, Bandarban, Bangladesh

Abstract/ Executive Summary:

A field experiment was conducted at the central nursery of tea extension center of Bangladesh Tea Board in Bandarban during September to November, 2015 to determine the precise concentration of Indole Butyric Acid (IBA) on propagation through stem cuttings of tea. The experiment was laid out in a randomized block design with four treatments of IBA concentrations: 0 ppm (Control), 2000 ppm, 4000 ppm and 6000 ppm and each treatment was replicated four times. Basal part of tea (BT-2 variety) cuttings were dipped in different IBA concentrations for five seconds. Then these cuttings were dibbled into prepared soil mixture as rooting media. Cuttings were exposed to the rooting media for 90 days and then they all were removed carefully to assess the variation of establishment of cuttings. IBA treated cuttings showed better effect on the rooting rate, root length, root number, shoot length and survival rate compared to the control. It was noted that cuttings treated with 4000 ppm IBA produced the highest rooting rate 79.85%, the highest survival rate 57.60% and the highest root length of 4.85 cm followed by treatment 6000 ppm IBA. In case of all parameters, effects of the treatments 4000 ppm and 6000 ppm of IBA were statistically similar. Positive and strong correlation was observed both in rooting rate versus survival rate and root length versus shoot length in IBA treated cuttings. Thus, IBA at 4000 ppm concentration was recommended for establishment of stem cuttings of tea (BT-2 variety) in the nursery.

Keywords: Tea, Stem cuttings, IBA concentration

Location: Bangladesh Tea Board, Bandarban district

Start Date: 00-09-2015

End Date: 00-12-2015

Program Area: Variety and Species

Commodity: Tea

Objectives:

To determine the effect of IBA and to select the optimal concentration of IBA application on tea stem cuttings for raising tea plants in the nursery.

Methodology:

The experiment was carried out at the central nursery of tea extension center of Bangladesh Tea Board, Bandarban district during September to December of 2015. Sandy loam soil was collected and mixed with decomposed cow dung (one-fourth of total soil), nematicide and TSP before 20 days of cuttings dibbling. Thereafter the mixture was sieved through mesh net (8 No.) for the preparation of rooting materials. The 5x7 inches sized polythene sleeves were filled with soil mixture and arranged on bed under agro shed net tunnel which protect direct sun light. Suitable shoots were collected from healthy tea bushes of clone BT-2 variety from different garden of Bandarban. Uniform single node cuttings of 2.5-3.0 cm long from new shoots were used for the experiment. Typically three, sometimes four cuttings were made from each shoot, depending on the length of the internodes of the shoot. Prepared cutting were soaked in a disinfecting solution of fungicide Cupravit for 2–3 minutes just prior to application of growth regulators. Indole Butyric Acid (IBA) at the rate of 0, 2000, 4000, 6000 ppm were applied as treatments for experiment. The solutions were prepared by dissolving 0, 200, 400, 600 mg, respectively of IBA pure crystal in drops of (1.N) NaOH and diluted with water to make 100 mL of each concentration. The base portion (1.0-1.5 cm) of cuttings were soaked carefully in solution for 5 seconds. Then these treated cuttings were planted into polythene sleeves containing prepared soil mixture as rooting media at evening. The Randomized Block Design (RBD) was followed in the experiment in an arrangement with four replications having 20 cuttings in each replication under every treatments. The soil filled polythene sleeves with cuttings were lightly irrigated every day for first week of dibbling and then maintained one day interval for irrigation up to experimental period and besides this weeding was performed weekly. After 90 days of cuttings dibbling, various growth parameters were evaluated against the treatments. For that purpose, cuttings were carefully uprooted and medium particles adhered to roots were removed carefully by hand. Data were collected on growth parameters i.e., rooted cutting, root number per cutting, length of root and shoot, survival rate of cutting and simple arithmetic calculation was performed to get consolidated figure on blocks and treatments for each parameters. The data were analyzed using General Linear Model of SPSS software (version 15) and analysis of variance (ANOVA) was performed to determine the effect of different concentration of IBA on dependent variables. Means on selected parameters were compared using Tukey’s test at 5% level of significance.

Source of Information: The Agriculturists 14 (2):124-129 (2016

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The results indicated that IBA treated cuttings demonstrated better results as compared to control in all agronomic parameters. The vegetative propagation of tea through stem cuttings with IBA treatment can be applied on commercial scale for rapid multiplication. IBA application in tea cutting at 4000 ppm produced the highest rooting rate (79.85%), survival rate (57.60%) and root length (4.85 cm) and also at 6000 ppm produced the highest root number per cutting (8.33) and highest shoot length (12.98 cm). Results to the context of all parameters of tea cutting establishment were found statistically similar in case of 4000 ppm and 6000 ppm IBA concentration. Hence from the results, it can be inferred that IBA had a positive effect on the establishment of stem cuttings of tea (BT-2 variety) and 4000 ppm IBA can be applied for raising better plants from stem cuttings in the tea nursery.

Knowledge Management: Journal