One hundred seventy vegetable samples viz., brinjal, cabbage, hyacinth bean, yard long bean, bitter gourd, snake gourd, pointed gourd, okra and tomato were collected from local market of eight different regions namely Comilla, Gazipur, Jessore, Norsingdi, Tangail, Dhaka, Jamalpur and Rangpur. The weight of the sample of each vegetable was 1 kg. Samples were collected according to regulation made in the ''Guidelines for the control of pesticide residues in foods" (Anonymous, 1996), which incorporate the EU directive (Anonymous, 1979) and Codex recommendations (Anonymous, 1993) regarding sampling. Collected samples were kept in “Chilled box” and carried to the laboratory within the quickest time and stored in refrigerator (-200C) until analysis. The standard for diazinon, malathion, quinalphos, fenitrothion, chlorpyriphos, acephate, fenvalerate and cypermethrin were obtained from Sigma-Aldrich Laborchemikalien, Seelze, Germany via Bangladesh Scientific Pvt. ltd. Dhaka, Bangladesh. Standards of all the insecticides contained >99.6% purity. Chromatograms obtained from standard were used to compare residue of the vegetable samples. Extraction, separation and clean up of vegetable samples- The collected vegetable samples (250 g) were chopped by knife on white board and mixed well. A sub sample of 20g was taken into a wide mouth jar with the help of a spatula then 100 ml of hexane was added to it. Sodium sulphate (Na2SO4) was also added with sample until water was removed from the sample. The mixture was then macerated with high-speed homogenizer (Ultra-turrax, IKA T18 basic, Germany) for 2 minutes. The homogenized material was then poured into 250 ml conical flask and placed into the shaker (Refrigerated Shaker, Rexmed, Sweden) for 12 hrs continuous shaking. After shaking, the slurry was filtered through what man filter paper no.40 and a buchner funnels with suction. The flask and filter cakes were rinsed with 25 ml of hexane each. The filtrate was then transferred into 250 ml round bottom flask and was dried to around 5-7 ml by evaporation using a rotary vacuum evaporator (Laborota-4001, Heidolph, Germany) at 35-40ºC. Then, the concentrate filtrate was collected in a centrifuge tube adjusted at 10 ml volume which was then centrifuged at 16500 rpm for 10 minutes with laboratory refrigerated centrifuges (Sigma-3K30, Germany). After centrifuge, supernatant was collected and cleaned up by Super Phase Extraction (SPE) cartridge. Then the final volume was kept in 10 ml volumetric flask. Before injection, this volume was again cleaned up by High Performance Liquid Chromatograph (HPLC) filter (0.2 PTFE) which was ready for injection in Gas Chromatograph. Detection and quantification of insecticide residue in samples- The concentrated extracts were subjected to analysis by GC-2010 (Shimadzu) with Flame Thirmionic Detector (FTD) for detection of organophosphorus (diazinon, quinalphos, acephate, chlorpyriphos, fenitrothion and malathion) insecticide. For detection of pyrethroid (cypermethrin, fenvalerate) each sample extract was again analyzed with Electron Capture Detector (ECD). The capillary column used was AT-1, length 30 m, ID 0.25mm and film thickness 0.25µm in case of both detectors. Helium was used as carrier and make up gas in FTD and in case of ECD, it was Nitrogen.