Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Phytoextraction Efficiency of Lead by Arum (colocasia Esculental.) Grown in Soil

Md. Shoffikul Islam
Department of Soil Science, University of Chittagong, 4331 Chittagong, Bangladesh

Md. Abul Kashem
Department of Soil Science, University of Chittagong, 4331 Chittagong, Bangladesh

Khan Towhid Osman
Department of Soil Science, University of Chittagong, 4331 Chittagong, Bangladesh

Abstract/ Executive Summary:

Background and Objective: Lead (Pb) contamination of agricultural soil and water via anthropogenic sources have resulted serious problems in the food chain and consequently the health of organisms, including man. Phytoremediation has been touted as a promising alternative for the generally expensive and disruptive conventional remediation techniques to clean up Pb contaminated soil. Therefore, the aim of this study was to investigate the Pb tolerance and phytoextraction efficiency of arum (Pani Kachu; Colocasia esculenta L.) grown in soil spiked with different levels of Pb. Materials and Methods: The plant was grown for 105 days in soils spiked with Pb at the levels of 0, 300, 600 and 1200 mg kg^–1. The plant parts were digested with HNO₃-HClO₄ (3:1) acid mixture. Lead in the digests was measured using atomic absorption spectrophotometer. Results: Dry weight of plant parts was not affected by any levels of Pb concentration while concentration of Pb increased in plant parts with the increase of Pb levels in soil. In shoots, concentration of Pb was 7748 mg kg^–1 at the highest level of Pb concentration (1200 mg kg^–1) in soil. This concentration (1200 mg kg^–1) did not cause any growth retardation of arum plant which indicated that arum was a metal hyperaccumulator plant. However, translocations of Pb in arum parts at this high level was 62% of total Pb which indicated that a major portion of metal was translocated from roots to shoots. Transfer Factor (TF) greater than one as found in the present experiment confirmed the hyperaccumulation characteristics of arum for Pb in soil media. Conclusion: This study indicated that the growth of arum was unaffected by application of any levels of Pb in soil. The Pb concentration in the shoots of arum without growth retardation and TF of Pb in arum indicated that this plant was a suitable candidate for the phytoremediation of Pb contaminated soil.

Keywords: Metal, Contamination, Hyperaccumulator, Arum, Phytoextraction, Toxicity, Phytoremediation, Soil

Location: Department of Soil Science of the University of Chittagong, Bangladesh

Start Date:

End Date:

Program Area: Risk Management in Agriculture

Commodity: Aroids/Arum

Objectives:

To investigate the Pb tolerance and phytoextraction efficiency of arum grown in soil spiked with different levels of Pb were investigated.

Methodology:

A surface (0-15 cm) sandy loam soil was collected from the crop field near the net house of the Department of Soil Science of the University of Chittagong, Bangladesh. The collected soil was air dried, ground and passed through a 4 mm sieve for using in pot experiment. For laboratory analysis, a sub sample was passed through a 2 mm sieve and stored. Soil pH of 5.0 was measured in a 1:2.5 soil/water suspension. Soil Organic Carbon (OC) of 0.4% was determined by wet oxidation method. Cation Exchange Capacity (CEC) of 4.1 cmol kg^–1 was determined by extraction with 1 M NH₄OAc (pH 7.0). The hydrometer method was used for the particle size distribution. The soil had 67.9% sand, 12.7% silt and 19.4% clay. The pot experiment was conducted under natural light condition in a net house of the Department of Soil Science at the University of Chittagong, Bangladesh. Moist soil equivalent to 7 kg dry mass was placed in a 12 L plastic pot after mixing 0, 300, 600 and 1200 mg kg^–1 of Pb as Pb(NO₃)₂ (ACS grade, Sigma-Aldrich Co.). In each pot, N (as (NH₄)₂ SO₄), P (as KH₂PO₄) and K (as KCl) was added as 100 mg kg^–1 soil as recommended by SRDI Report of Land and Soil Resource Use. Three replications of the treatment were set out in a complete randomized block design. Two healthy and uniform size plantlets of arum (Colocasia esculenta L.) were transplanted in each pot. After one month, one plantlet was left in each pot. From 40 days of growth, water was added daily approximately up to the field capacity during the growth period. Plants were harvested after 90 days of growth following transplanting. Plant roots were carefully removed from each pot and washed thoroughly to get rid of adhering soil particles followed by quick wash of deionized water. The fresh weights of plants were measured. Then the plants were separated into leaves, stems, stolons, rhizomes and roots and their fresh weights were measured. After drying in an oven at 68°C for 72 h, their dry weights were also measured. The plant parts were grounded using a stainless steel grinder and were then digested with HNO₃-HClO₄ (3:1) acid mixture. Lead in the digests was measured using atomic absorption spectrophotometer (Agilent Technologies, 420 AA, Australia). Reagent blanks were processed to ensure Pb was not added during sample preparation. The TF of Pb was measured by dividing the concentration of Pb in shoots to those in roots of arum grown in soil culture. All results are presented on Dry Weight (DW) basis. The results obtained were subjected to one way analysis of variance using Minitab program.

Source of Information: International Journal of Soil Science, 11: 130-136. 2016

Article Link (Online Journal): URL: https://scialert.net/abstract/?doi=ijss.2016.130.136, DOI: 10.3923/ijss.2016.130.136

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The results of this study indicate that the growth of arum is unaffected by application of any levels of Pb in soil. The concentrations of Pb found in shoot tissue and the TF of Pb in arum plant indicates that this plant has an excellent potential for Pb phytoextraction. This implies that arum is a potential candidate for the reclamation of Pb contaminated soil and water.

Knowledge Management: Journal