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Cardioprotective Molecule and Bioactive Compounds of Some Selected Vegetables Available in Bangladesh

Md. Arif Sakil
Department of Biochemistry & Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Mohammed Arif Sadik Polash
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Tahjib-Ul- Arif
Department of Biochemistry & Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Afzal Hossain
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Alamgir Hossain
Department of Crop Botany, Bangladesh Agricultural University,

Abstract/ Executive Summary:

Cardiovascular disease (CVD) is one of the leading causes of death and morbidity as well as imposing a huge economic burden at both country and household level. Upto 90% cardiovascular disease may be preventable if established risk factors are avoided. In this context, dietary nitrate from vegetables was highlighted as a potential candidate for cardio protection. Hence, by supplying dietary nitrate and other bio-active compounds to ease the risk of cardiovascular disease, present research work was carried out. Among the tested vegetables, the highest nitrate content (745 mg/100g) was determined in Indian spinach. In addition, Indian spinach also possesses more chlorophyll (150mg/100g), beta carotene (40mg/100g) and lycopene (34mg/100g) than other tested vegetables in the current study. In case of anti-oxidant content, Indian spinach showed the highest (103mg/100g) vitamin C content. Taken all together, Indian spinach may be a good and cheapest source of cardio protective molecule (nitrate) bioactive compounds to avoid risk of cardiovascular disease.

Keywords: Cardio protective molecule, Bioactive compounds, Antioxidant, Diphenylamine, Dietary nitrate

Location: Food Biochemistry laboratory, Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh

Start Date: 00-08-2017

End Date: 00-10-2017

Program Area: Quality and Nutrition

Commodity: Vegetables

Objectives:

The current research work was designed to determine the content of nitrate, bioactive compound and antioxidant of selected vegetables available in Bangladesh.

Methodology:

Mustard, leaf mustard, radish, cabbage, lettuce, Indian spinach, spinach, coriander leaf, water spinach, Amaranth, jute leaf, gourd leaf and turnip were the experimental materials of this study which were collected at 30 days after sowing (DAS) from different cultivated area of Mymensingh, Bangladesh. Details of experimentation The experiment was conducted in Food Biochemistry laboratory, Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh from August 2017 to October 2017. Among the listed vegetables six vegetables (Leaf mustard, cabbage, coriander leaf, lettuce, Indian spinach and spinach) were selected by performing Diphenylamine (DPA) field kit test (Roberts, 1994) based on intensity of blue color. Then selected vegetables were analyzed for nitrate (NO_3–), bio-active compounds, anti-oxidant (Vitamin C). Each sample was analyzed three times. Nitrate (NO₃_-) content: Nitrate (NO₃_-) content from fresh vegetables was measured by “Improved Diphenylamine-based Spectrophotometric Method” (Omar et al., 2013). Nitrate calibration curve was prepared by using potassium nitrate (KNO₃) as standard. Content of bioactive compounds: The leaves of selected vegetables (1 g) were extracted in 10 ml of chilled acetone solution in dark. After centrifugation at 4000 rpm for 10 minutes at room temperature the absorbance of supernatants was taken at 453, 505, 645 and 663 nm wave length. Contents were calculated according to the equation used in Barros et al., (2010). Ascorbic acid content: Ascorbic acid was determined following a procedure previously described by Xaio et al. (2012), with 2.6-dichloroindophenl and measuring the content by titrimetric method. The results were expressed as mg of ascorbic acid per 100g of fresh weight. Data analysis The collected data were statistically analyzed by using Minitab 17 statistical Computer Package Programmer in accordance with the principles of Completely Randomized Design. Duncan’s Multiple Range Test (DMRT) was used to compare variations among the treatments

Source of Information: J Bangladesh Agril Univ 16(1): 82–87, 2018 ISSN 1810-3030 (Print) 2408-8684 (Online)

Article Link (Online Journal): doi: 10.3329/jbau.v16i1.36485

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Based on results, Indian spinach contained the highest amount of nitrate which may acts as a vasodilator and helps in reduction of blood pressure and cardiovascular diseases after bioconversion of nitrate as NO in our body. Further research is needed to clarify the issue by using human subjects and/or lab animals

Knowledge Management: Journal