Agricultural Research Management Information System

  • Home
  • Research Summary
    • All
    • Government Organization
      • Agriculture Training Institute, Ishwardi, Pabna
      • Bangabandhu academy for poverty alleviation and rural development (BAPARD)
      • Bangabandhu Sheikh Mujibur Rahman Science & Technology University
      • Bangladesh Bureau of Statistics
      • Bangladesh Institute of Health Sciences
      • Bangladesh Institute of Tropical & Infections Diseases (BITID)
      • Bangladesh Meteorological Department
      • Bangladesh National Herbarium
      • Bangladesh Space Research and Remote Sensing Organization
      • Bangladesh Technical Educational Board
      • Barind Multipurpose Development Authority
      • Central Cattle Breeding Station
      • Department of Agriculture Extension
      • Department of Fisheries
      • Department of Livestock Services
      • Department of Youth Development
      • Dhaka Medical College
      • Geological Survey of Bangladesh
      • Institute of Epidemiology, Disease Control & Research
      • Jatiya Kabi Kazi Nazrul Islam University
      • Khulna Govt. Women College
      • Livestock Training Institute
      • Local Government Engineering Department
      • Ministry of Agriculture
      • Ministry of Environment and forest
      • Ministry of Fisheries and Livestock
      • Ministry of Labour & Employement
      • Ministry of Land
      • Ministry of Public Administration
      • Ministry of Textiles and Jute
      • Ministry of Water Resources
      • Ministry of Youth and Sports
      • National Agricultural Training Academy
      • National institute of preventive and social medicine
      • National Mushroom Development and Extension Centre
      • Pabna University of Science and Technology
      • Seed Certification Agency
      • Shaheed Suhrawardy Medical College
      • Sheikh Hasina University
      • University Grants Commission
      • Youth Training Centre
    • Autonomous/Semi-gov Org
      • Bangladesh Academy for Rural Development
      • Bangladesh Agricultural Development Corporation
      • Bangladesh Atomic Energy Commission
      • Bangladesh Council of Scientific and Industrial Research
      • Bangladesh Fisheries Development Corporation
      • Bangladesh Institute of Development Studies
      • Bangladesh Institute of Management
      • Bangladesh Milk Producers Cooperative Union Limited
      • Bangladesh Water Development Board
      • BIRDEM
      • Center for Environmental and Geographic Information Services
      • Hortex Foundation
      • Institute of Water Modeling
      • National Institute of Biotechnology
      • River Research Institute
      • Rural Development Academy
    • NARS
      • Bangladesh Agricultural Research Council
      • Bangladesh Agricultural Research Institute
      • Bangladesh Fisheries Research Institute
      • Bangladesh Forest Research Institute
      • Bangladesh Institute of Nuclear Agriculture
      • Bangladesh Jute Research Institute
      • Bangladesh Livestock Research Institute
      • Bangladesh Rice Research Institute
      • Bangladesh Sericulture Research and Training Institute
      • Bangladesh Sugarcrop Research Institute
      • Bangladesh Tea Research Institute
      • Bangladesh Wheat and Maize Research Institute
      • Cotton Development Board
      • Soil Resource Development Institute
    • Public University
      • Ahsanullah University of Science and Technology
      • Bangabandhu Sheikh Mujibur Rahman Agricultural University
      • Bangamata Sheikh Fojilatunnesa Mujib Science and Technology University
      • Bangladesh Agricultural University
      • Bangladesh Open University
      • Bangladesh University of Engineering and Technology
      • Bangladesh University of Professionals
      • Bangladesh University of Textiles
      • Barisal Government Veterinary College
      • Begum Rokeya University
      • Chittagong University of Engineering and Technology
      • Chittagong Veterinary and Animal Science University
      • Comilla University
      • Dhaka University of Engineering & Technology
      • Dinajpur Government Veterinary College, Dinajpur
      • Gono Bishwabidyalay
      • Hajee Mohammad Danesh Science and Technology University
      • Islamic University, Kushtia
      • Jagannath University
      • Jahangirnagar University
      • Jessore University of Science and Technology
      • Jhenaidha Government Veterinary College
      • Khulna Agricultural University
      • Khulna University
      • Khulna University of Engineering & Technology
      • Mawlana Bhashani Science and Technology University
      • Millitary Institute of Science and Technology
      • National University
      • Noakhali Science and Technology University
      • Patuakhali Science and Technology University
      • Rajshahi University of Engineering and Technology
      • Shahjalal University of Science & Technology
      • Sher-e-Bangla Agricultural University
      • Sylhet Agricultural University
      • Sylhet Government Veterinary College
      • University of Barisal
      • University of Chittagong
      • University of Dhaka
      • University of Rajshahi
    • Private University
      • Asian University of Bangladesh
      • Atish Dipankar University of Science and Technology
      • BGC Trust University Bangladesh
      • BGMEA University of Fashion & Technology (BUFT)
      • BRAC University
      • City University
      • Daffodil International University
      • East West University
      • Exim Bank Agricultural University
      • Gana Bishwabiddalaya
      • Hamdard University
      • Independent University, Bangladesh
      • International Islamic University Chittagong
      • International University of Business Agriculture and Technology
      • Islamic University of Technology
      • Leading University, Sylhet
      • North South University
      • Premier University
      • Primeasia University
      • Private University
      • SOAS, University of London
      • Southeast University
      • Stamford University
      • State University of Bangladesh
      • The Millenium University
      • University of Asia Pacific
      • University of Development Alternative
      • University of Information Technology and Sciences
      • University of Liberal Arts Bangladesh
      • University of Science and Technology, Chittagong
      • World University
    • INGO/IO/NGO/Private Org
      • ACI Limited
      • Agricultural Advisory Society (AAS)
      • Apex Organic Industries Limited
      • Arannayk Foundation
      • Bangladesh Academy of Sciences
      • Bangladesh Centre for Advanced Studies
      • Bangladesh Institute of Social Research
      • Bangladesh Science Foundation
      • Bangladesh Unnayan Parishad
      • BAPA
      • BRAC
      • CARE Bangladesh
      • CARITAS
      • Centre for Environmental Geographical Information System
      • Centre for Policy Dialogue (CPD)
      • Creative Conservation Alliance
      • Dhaka Ahsania Mission
      • Dwip Unnayan Sangstha
      • EMBASSY OF DENMARK, BANGLADESH
      • Energypac Limited Bangladesh
      • FAO- Bangladesh
      • FIVDB
      • ICDDRB, Mohakhali, Dhaka-1212
      • iDE Bangladesh
      • Innovision Consulting Private Ltd.
      • International Center for Climate Change and Development
      • International Centre for Integrated Mountain Development
      • International Development Research Centre
      • International Fertilizer Development Center, Bangladesh
      • International Food Policy Research Institute
      • International Maize and Wheat Improvement Centre
      • International Potato Center
      • IRRI- Bangladesh
      • IRRI-Philippines
      • Ispahani Agro LTD
      • IUCN, Bangladesh
      • Krishi Gobeshina Foundation
      • Lal Teer
      • Mennonite Central Committee
      • Metal (Pvt.) Ltd
      • Modern Herbal Group
      • Palli Karma-Sahayak Foundation
      • Practical Action Bangladesh
      • Proshika
      • RDRS Bangladesh
      • RIRI-Philippines
      • Rothamsted Research
      • SAARC Agricultural Centre
      • SAARC Meteorological Research Centre
      • Social Upliftment Society
      • South Asia Enterprise Development Facility
      • Square Pharmaceuticals Ltd.
      • Supreme Seed
      • Transparency International Bangladesh
      • Unnayan Onneshan
      • USAID
      • Water Resources Planning Organization
      • Winrock International
      • World Bank
      • World Food Program
      • World Vegetable Center
      • WorldFish Centre, Bangladesh
    • Foreign University
      • Asian Institute of Technology
      • Auckland University of Technology
      • Australian National University
      • Bidhan Chandra Krishi Viswavidyalaya
      • BOKU-University of Natural Resources and Applied Life Sciences
      • Cranfield University
      • Curtin University
      • Foreign University/ Institute
      • Hiroshima University
      • Hokkaido University
      • Huazhong Agricultural University
      • International Islamic University, Malaysia
      • Kagawa University
      • Kangwon National University
      • Kochi University
      • Kyoto University
      • Kyushu University
      • Ladoke Akintola University of Technology
      • Murdoch University
      • Nagoya University
      • NOAA-CREST, CCNY
      • Royal Veterinary and Agricultural University
      • San Diego State University
      • Shinshu University
      • Tottori University
      • United Nations University
      • University Malaysia Kelantan
      • University Malaysia Pahang
      • University Nova de Lisboa
      • University of Alberta
      • University of Bremen
      • University of Bremen
      • University of Calgary
      • University of california
      • University of Greenwich
      • University of Hamburg, Hamburg
      • University of Hannover
      • University of Hawaii
      • University of Helsinki, Finland
      • University of Kalyani
      • University of Leeds
      • University of Liverpool
      • University of Malaya
      • University of Milan
      • University of New England
      • University of Philippines
      • University of Plymouth
      • University of Queensland
      • University of Reading
      • University of Southampton
      • University of Texas
      • University of the Punjab
      • University of Tokyo
      • University of Toronto
      • University of Wales
      • University of Washington
      • University of Wollongong
      • University Putra Malaysia
      • University Sains Malaysia
  • Search
    • Search by Keyword
    • Search by Organization
    • Search by Program Area
    • Search by Commodity/Non-commodity
    • Search by Funding Source
    • Search by Researcher
    • Custom Search
    • On-going Research
  • About Us
    • ARMIS
    • Brochure
  • Contact Us
    • BARC Personnel
    • ARMIS Personnel
    • Feedback
  • Report
    • All
    • By Organization
      • Bangladesh Agricultural Research Council
      • Bangladesh Agricultural Research Institute
      • Bangladesh Fisheries Research Institute
      • Bangladesh Forest Research Institute
      • Bangladesh Institute of Nuclear Agriculture
      • Bangladesh Jute Research Institute
      • Bangladesh Livestock Research Institute
      • Bangladesh Rice Research Institute
      • Bangladesh Sericulture Research and Training Institute
      • Bangladesh Sugarcrop Research Institute
      • Bangladesh Tea Research Institute
      • Bangladesh Wheat and Maize Research Institute
      • Cotton Development Board
      • Soil Resource Development Institute
    • Research Trend Analysis
  • User Request
  • Data Input
  • Help
    • Operation Manual
      • PDF
      • Video
    • Program Area & Commodity
  • We have reached 37600 number of research entries at this moment.
    • Logout

Research Detail

  1. Home
  2. Research
  3. Detail
Fatema Ahmmed
Fisheries and Marine Resources Technology, School of life science, Khulna University, Bangladesh

Mirja Kaizer Ahmmed
Feculty of Fisheries, Chittagong Veterinary and Animal Sciences University, Bangladesh

Md. Saifuddin Shah
Fisheries and Marine Resources Technology, School of life science, Khulna University, Bangladesh

Ghausiatur Reza Banu
Fisheries and Marine Resources Technology, School of life science, Khulna University, Bangladesh

The present study was conducted to study in-vitro the antagonistic effect of Lactobacillus spp. against the pathogenic bacterial vibrio harveyi on shrimp. For this purpose, shrimp samples were collected from three different Ghers at Batiaghata upazilla, Khulna. Gills and intestines were taken out from the samples to identify the load of Lactobacillus spp. and Vibrio spp. The results revealed that the load of Lactobacillus spp. was found more than Vibrio spp. both in gills and intestines; the gills also contained higher load of Vibrio spp. than in the intestines. V. harveyi was separated from the isolated Vibrio spp. with different types of biochemical tests: Gram stain, Motility test, Indole test, VP test, MR test, Arginine dihydrolase, Salt tolerance test, growth at different temperature ranges and colony color on TCBS agar media. The isolated V. harveyi was subjected for in-vitro test. In in-vitro challenge test, the potential antagonistic effect of Lactobacillus spp. against V. harveyi was gradually obtained at 0, 4th, 8th, 12th hour of treatments. Interesting finding was that, with the time,  the load of V. harveyi was reduced gradually and the lowest load was obtained after 12 hours of probiotic inoculation. The present study revealed an excellent in-vitro antagonistic probiotic effect of Lactobacillus spp. on V. harveyi. Therefore the result suggested that probiotic treatment might be an effective alternative to the use of antibiotics in treatments of bacterial diseases in shrimp aquaculture.

  Lactobacillus, Antagonistic effect, Aquaculture
  Batiaghata upazilla, Khulna, Bangladesh
  
  
  Crop-Soil-Water Management
  Shrimp, Virus

To find out the beneficial bacteria (Lactobacillus spp.) from shrimp for possible use as probiotics on V. harveyi infected shrimp.

The present study was conducted on three ghers of Batiaghata upazilla, Khulna. The ghers were randomly selected for collecting shrimp samples. Nine (9) specimens of shrimps, Paeneus monodon (8.10cm- 12.4cm in length and 13.12g-22.6g in weight) were collected from three ghers of the study area. The target organs (gill and intestine) of the samples were separated aseptically and weighed in electric balance. Organs (gills weight: 0.22g-0.34g and weights of intestinal tracts: 0.13g-0.25g.) were taken into eppendorf tubes with peptone water  for isolating Lactobacillus spp. and alkaline saline peptone water was used for isolating Vibrio spp. Then they were homogenized using tissue homogenizer. Then homogenized solutions were centrifuged at 3000 rpm for 3 minutes. After centrifugation the supernatant liquid portion was collected with a micropipette and taken into eppendorf tube and preserved in deep freeze. Lactobacillus spp. was isolated from gills and intestinal tracts of the collected shrimp samples. The stock solution  was diluted (tenfold dilution) with peptone water.  0.1 ml suitable dilution  of each stock solution was inoculated in MRS Lactobacillus agar media and incubated at 370C temperature for 24-48 hours. After incubation at 370C temperature for 24–48 h, cream-colored colonies with yellow halos were collected and preserved for further experiment. Vibrio spp. was isolated from the gills and intestinal tracts of the collected shrimp samples from study area. ISO method was followed for isolating Vibrio spp. 0.1ml stock solution of each gill and intestinal tract was taken and mixed with 0.9ml alkaline saline peptone water (ASPW) in sterilized test tubes. Then the mixture was incubated at 37 °C for 6 ±1 hr. After that, whole culture of each test tube obtained in first selective enrichment was taken and transferred into other test tubes each containing 10 ml ASPW. Then the solution was incubated at 41.5 °C for 18 ± 1 hr. This was the second selective enrichment. Then serial dilution (tenfold) was done and 0.1ml suitable dilution of each culture was inoculated in thiosulfate citrate bile and sucrose (TCBS) agar plates. Then the inoculated TCBS agar plates were incubated at 37 °C. After 24 h ± 3 h of incubation, the plates were examined for the presence of typical colonies of presumptive Vibrio spp. (ISO/TS 21872-1, 2007). The identification of the Vibrio harveyi colonies was done by performing various biochemical tests viz, motility test, methyl red test, VP test, arginine dihydrolase, indole ring test, Salt tolerance test (0%, 1%, 3%, 5%, 7%, and 10%) and growth at ( 4°C, 28°C, 37°C, 55°C). The isolates were identified at the species level with the use of biochemical key. Gram stain was done to identify the gram positive and gram negative bacteria (Cowan and Steel’s, 1993). The Indole test was performed in a 48h culture in peptone water adding about 1ml ether; shake; run 0.5 ml Ehrlich’s reagent down the side of the tube. A red color in the solvent indicates positive reaction. The VP test was performed after completion of the methyl red test adding 0.6ml 5% α-naphthol solution and 0.2 ml 40% KOH aqueous solution; shake well, slope the tube, and examine after 15 min & 1 h. A positive reaction is indicated by a strong red color. The MR test inoculated the isolated bacteria with buffered glucose broth & incubated at 37°C for 48h. After incubation adding a few drops of methyl red solution to the culture, read immediately. A red color represents a positive test. The motility test stabbed inoculates tubes of motility medium to a depth of about 5 mm. Incubate at or below the optimum growth temperature. Motile organisms migrate throughout the medium, which becomes turbid; growth of non-motile organisms is confined to the stab inoculum. This test was done on nutrient agar media supplemented with varying amounts of NaCl (0%, 1%, 3%, 6%, 8%, and 10%). This was performed to study the salt tolerance range of the isolated species and the optimum concentration, once determined was supplemented in various media required to test their biochemical properties. The isolated Vibrio spp. was kept in incubator after inoculation at subsequent interval at temperature 40C, 280C, 370C, 550C and checked for their survivability and colony formation obtained at 280C and 370C. After identification of Vibrio harveyi by biochemical test one colony of V. harveyi was taken into eppendorf tube by isolating loop into 0.9ml peptone water. Prepared stock solution of V. harveyi (ISO/TS 21872-1, 2007). The stock solution was diluted (tenfold dilution) with peptone water (James and Hirsch, 1960) and prepared test solution of V. harveyi. Then 0.5 ml isolated probiotic solution was separately mixed with 0.5ml of test solution (V. harveyi). 0.1ml suitable dilution of the mixer solution was inoculated in TCBS agar media after at subsequent intervals of 4 hour up to 12hour. This procedure was done for 2 times. Test solution of V. harveyi ; without probiotic was also inoculated in TCBS agar media at 0 hour, 4th hour, 8th hour and 12th hour subsequently. All the inoculated TCBS agar plates were incubated at 370 C for 24± 3 hours. Standard plate count was done after incubation. Collected data were stored, explored and analyzed using Microsoft Excel (Microsoft office, 2007) and Statistical Package for the Social Sciences (SPSS version 16.0; SPSS, Inc., Chicago, IL). Independent sample t-test was applied to address the differences between the treatment and control at 1% significance level using SPSS (version 16.0). 

  Res. Agric. Livest. Fish. Vol. 5, No. 1, April 2018 : 127-135.
  
Funding Source:
1.   Budget:  
  

From this in-vitro challenge test with Lactobacillus spp. it was found that it significantly reduced the V. harveyi load of the selected shrimp samples. From this in-vitro test it was proved that Lactobacillus spp., had the inhibitory property of a biocontrol agent for use in control of shrimp pathogens and might be useful for replacing the commercial antibiotics. The present investigation also clearly demonstrated that, putative probionts isolated from infected shrimp possesses an excellent antibacterial effect and could be applied in aquaculture operation as an effective tool of treatment to prevent and cure shrimp diseases caused by V. harveyi. On the basis of the results obtained in this work, it could be concluded that Probiotics, as ‘bio-friendly agents’ such as Lactobacillus spp., could be introduced into the culture environment to control and compete with pathogenic bacteria as well as to promote the growth of the cultured organisms.

  Journal
  


Copyright © 2025. Bangladesh Agricultural Research Council.