Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Efficacy of Some Botanicals Against Pulse Beetle, Callosobruchus Maculatus(fab.) on Stored Greengram, Vigna Radiata

M. A. MIAH
Department of Entomology, Patuakhali Scienceand Technology University, Dumki, Patuakhali-8602;

M. R. ALI
Department of Entomology, Sher-e-Bangla Agricultural University, Dhaka-1207

A. HUSNA
Department of Biotechnology, Patuakhali Scienceand Technology University, Dumki, Patuakhali-8602;

M. M. I. MOLLAH
Department of Entomology, Patuakhali Scienceand Technology University, Dumki, Patuakhali-8602;

Abstract/ Executive Summary:

Insecticidal efficacy of some botanicals, Neem (Azadiracta indica) oil, Castor (Ricinus communis) oil, Camphor (Cinamomum camphora), Neem leaf powder,Castor leaf powder and Bankolmi (Ipomoea sepiara) leaf powder were tested on green gram (Vigna radiata) against pulse beetle, Callosobruchus maculatusinthe laboratory. Among the botanicals, Camphor @ 2 gm kg^-1 seeds, Neem oil @8 ml kg^-1seeds and Castor oil @ 8 ml kg^-1seeds performed best in respect of per cent reduction of oviposition, egg hatching, larval and pupal development,grain damage and grain content loss while partial reduction occurred by Neem leaf powder, Castor leaf powder and Bankolmi leaf powder. The order of effectiveness among the botanicals was Camphor > Neem oil > Castor oil >Neem leaf powder > Castor leaf powder > Bankolmi leaf powder. Considering the germination of seeds, there was no significant effect of Camphor, Neem oil and Castor oil treated seeds, while considerable germination reduced in Neem,Castor and Bankolmi leaf powder at 5% w/w treated seeds. It indicated that leaf powder of different botanicals was less effective against pulse beetle development compared to the different oils of botanicals.

Keywords: Efficacy, Botanicals, Callosobruchus maculatusand, Green gram

Location: Dept. of Entomology, Sher-e-Bangla Agricultural University, Dhaka

Start Date: 00-03-2008

End Date: 00-10-2008

Program Area: Pest Management

Commodity: Insects, Extract (plant, seed)

Objectives:

The present study was undertaken to screen some botanicals with a view to explore the insecticidal properties against pulse beetles in stored green gram

Methodology:

The efficacy of some botanicals was evaluated in storage condition in the laboratory under the Dept. of Entomology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during the period from March to October, 2008. Test insects (Pulse beetle, Callosobruchus maculatus) used in the present investigation were obtained from stock cultures in the laboratory of the Department of Entomology, Sher-e-Bangla Agricultural University. Stock culture of C. maculatus was maintained on green gram seeds in plastic jars covered with cloth in the same laboratory. One hundred pairs of C. maculatus were introduced into a plastic container (12 cm dia. 14 cm height) containing 200 gm of deinfested seeds. The mouth of the containers was covered with perforated plastic lids. The insects were allowed to mate and lay eggs for 24 hours, after which sieving was done to separate the adult beetles. The green gram seeds with eggs left on the sieve were kept for adult emergence. One-day-old (0-24 hours) adults were used for study. The rearing procedure was repeated indifferent batches to ensure continuous supply of the adults with required eggs. Male and female beetles were sorted out under simple microscope by their antennal characteristics, size and shape of the body. Seeds of green gram (Vigna radiata Walp), variety Barisal Local (Local variety) were used for the present study as most susceptible hosts t oC. maculatusi.e. as food of pulse beetle. Seeds were cleaned and disinfested by keeping the seeds at 0^oC for 14 days prior to use. Six botanicals were used as test insecticides. Among them, Neem (Azadirachta indica) oil and Castor (Ricinus communis) oil were applied @ 8 ml/kg, leaf powder of Neem, Castor and Bankolmi (Ipomoea sepiara) were applied @ 5% w/w and Camphor (Cinamomum camphora) was used @ 2 g/kg. All the botanicals were tested against C. maculatuson the seeds of Barisal local variety as the most susceptible host. Among the tested botanicals, Neem oil, Castor oil and Camphor were bought from the local market whereas the leaves of the tested botanicals were collected from University campus. Collected leaves and seeds were dried under ambient room temperature (27°C-34°C) and then ground separately by a hand grinder and sieved through a 60-mesh sieve to get fine powder. Two hundred gram(200 g) of green gram seeds (Barisal local) were taken separately for each treatment in a round plastic pot (12 cm dia. 14 cm height) and specific botanicals were mixed thoroughly by hand shaking. Ten pairs (male and female) of one day old healthy adults of C. maculatus were released in each of the plastic pots. The plastic pot was covered with their lids and kept at room temperature (27°C-34°C)in the laboratory for 45 days. The developmental stages were also observed and the data were recorded. The weight of the grains was recorded after 50 days of release of pulse beetles. Germination tests were also done with randomly taken 100 green gram seeds from each of the treatment at the initial and after 50 days of treatment application to evaluate the treatment effects on the viability of the seeds and the tests were replicated three times. The seeds were placed in Petri-dish having water soaked blotting paper at the bottom. Then the Petri-dish containing green gram seeds were incubated at room temperature and germination was estimated by direct count. For recording data, 100 seeds for each treatment were collected randomly from each treated pot. From the experiment, data were calculated on different parameters. The experimental data were analyzed by ANOVA-1 in Completely Randomized Design (CRD) with three replications and the means were separated by DMRT.

Source of Information: Bangladesh j. entomol. (2013) 23(1), 11-20 ISSN 1021-1004

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From these findings it was revealed that Camphor @ 2 gm/kg seeds, Neem oil @8 ml/kg seeds and Castor oil @ 8 ml/kg treated seeds performed best in considering the percent reduction of egg deposition, egg hatching, larval and pupal development, damaged seed and grain content loss applied against C. maculatusinfesting Barisal local variety of green gram.The effectiveness of the treatments was found in the following order: Camphor > Neem oil) > Castor oil > Neem leafpowder > Castor leaf powder > Bankolmi leaf powder > Untreated control. From the findings it was revealed that there were no significant effects of Camphor, Neem oil and Castor oil in reducing the viability of green gram seeds.

Knowledge Management: Journal