Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Prevalence of Poultry Diseases in Winter Season in Chittagong and Efficacy of Challenge Vaccine Against Newcastle Disease

Md. Saiful Bari
Department of Dairy and Poultry Science, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4225, Bangladesh

Eaftekhar Ahmed Rana
Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4225, Bangladesh

Md. Harisul Abid
Department of Microbiology, Jhenaidah Government Veterinary College, Jhenaidah- 7300, Bangladesh

Nasima Akter
Department of Dairy and Poultry Science, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4225, Bangladesh

Md. Abu Sayeed
Department of Medicine and Surgery, Jhenaidah Government Veterinary College, Jhenaidah- 7300, Bangladesh

Abstract/ Executive Summary:

A study was undertaken to determine the prevalence of poultry diseases as well as the efficacy of challenge vaccine [ND killed (Lasota)] against Newcastle disease (ND) in commercial broiler and layer farms of Chittagong Metropolitan Area (CMA) during the period from December 2016 to February, 2017. A total of 134 birds were examined from 52 commercial broiler farms and 15 layer farms (two from each farm). The diseases of 67 farms were diagnosed based on the history, clinical signs and post-mortem findings and the efficacy of the ND killed (Lasota) vaccine was determined on the basis of hemagglutination inhibition (HI) test before and after vaccination. Sixty blood samples (20 from each of 3 broiler farms) and 48 eggs (16 from each of 3 layer farms) were collected and HI tests were performed. Among the 67 farms, the prevalence of colibacillosis, salmonellosis, necrotic enteritis, Newcastle disease, infectious bursal disease, mycoplasmosis, brooder pneumonia and mixed infections of colibacillosis and coccidiosis were 11.94%, 4.48%, 7.46%, 8.95%, 16.42%, 7.46%, 5.95% and 7.46%, respectively. Due to vaccination with ND killed (Lasota) vaccine at initial stage of infection the mortality rate reduced to zero and HI titre of the broiler farms increased as 5.40 to 7.55, 4.6 to 7.25, 4.65 to 7.70 and in the layer farms as 4.56 to 7.13, 5.0 to 7.81, 4.93 to 8.13. Due to ND challenge vaccine the initial HI titre level significantly (p<0.05) varies from post HI titer level within both broiler and layer farms.

Keywords: Blood, Eggs, HI titre, Vaccination, Newcastle disease

Location: Chittagong Metropolitan Area, Chittagong, Bangladesh

Start Date: 00-12-2016

End Date: 00-02-2017

Program Area: Animal Health and Management

Commodity: Poultry, Becteriocides

Objectives:

To reveal the prevalence of poultry diseases in winter seasons and efficacy of challenge vaccine against Newcastle disease in Chittagong region of Bangladesh.

Methodology:

The study was conducted at Chittagong Metropolitan Area (CMA) of Chittagong, Bangladesh. The laboratory work was done in Pathology laboratory, CVASU and Chittagong veterinary laboratory, Khulshi, Chittagong. A total of 134 birds brought from different farms of CMA to pathology laboratory, CVASU were examined from 52 commercial broiler farms and 15 layer farms (two from each farm). Postmortem examination of birds was done for diagnosis (Charlton, 2000) and treatment of poultry diseases. The study was conducted in winter season (December, 2016 to February, 2017). Poultry diseases were diagnosed on the basis of clinical history, clinical signs and postmortem findings. A total of 60 blood samples were collected from 3 commercial broiler farms; 20 samples from each of the farms. The serum was separated from the blood. HI titre was taken twice for the birds of each farm; one at the time of initial stage of ND infection when birds were vaccinated using ND killed vaccine (Lasota) and after 7 days of the vaccination. A total of 48 egg samples were collected from 3 commercial layer farms; 16 samples from each of the farms. The egg samples were kept in refrigerator before the test. HI test was performed two times for the birds of each farm; one at the time of vaccination (at initial stage of ND infection)) and another titer was taken after 7 days of the challenge (ND killed, Lasota) vaccination. The serum antibody titer and egg yolk titer was estimated by microtiter method using 4 hemagglutination units of antigens as recommended by the World Organization for Animal Health (OIE, 2012). All the laboratory and field data were recorded in Microsoft office excel 2007 data sheet. The data were then cleaned and transported to STATA 11.0 statistical software. Finally Summary statistics were done along with some descriptive analysis.

Source of Information: Res. Agric. Livest. Fish. Vol. 5, No. 2, August 2018 : 201-208

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Control of highly prevalent poultry disease like ND, IBD and coccidiosis in winter season should be emphasized. However, an excellent biosecurity as well as vaccination strategy can be reduced the current prevalence of poultry diseases. In case of ND in poultry flock, administration of challenge vaccine raised the protective antibody titer level in the flock immediately against ND when the flock was previously vaccinated with any short of ND vaccine. Ultimately, it reduced the mortality rate by establishing immunological defense against ND. So, further details and strategic study is recommended on this type of challenge vaccination.

Knowledge Management: Journal