Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Autolyzed Saccharomyces Cerevisiae as a Single Cell Protein for Broiler Diet

Moniruzzaman M
Poultry Biotechnology and Genomics Laboratory, Department of Poultry Science, Faculty of Animal Husbandry, Bangladesh Agricultural University, Mymensingh 2202

MBR Mollah
Poultry Biotechnology and Genomics Laboratory, Department of Poultry Science, Faculty of Animal Husbandry, Bangladesh Agricultural University, Mymensingh 2202

Abstract/ Executive Summary:

Microbial protein often called as single cell proteins (SCP) are becoming a potential alternative to conventional protein rich ingredients in poultry diet. An experiment was conducted to know the possibility of using Saccharomyces cerevisiae derived SCP in broiler diet. A total of 96 male Ross-308 day old chicks were assigned to four diet comprising control (0% replacement), 25% replacement, 50% replacement and 100% replacement of protein concentrate with autolyzed S. cerevisiae. Each group had 6 replicates of 4 chicks in each. Body weight, body weight gain, daily weight gain, feed intake and feed conversion ratio were not affected (P>0.05) in case of 25% and 50% replacements. However, 100% replacement had resulted numerically negative effect as compared to control group. Dressing percentage and other carcass characteristics were not affected (P>0.05) in the replacement groups. The results of this study indicated that autolyzed S. cerevisiae might be used to replace protein concentrate at 25% to 50% level in broiler diet without affecting growth and carcass quality.

Keywords: Saccharomyces cerevisiae; Protein concentrate; Broiler; Single cell protein

Location: Bangladesh Agricultural University Poultry Farm, Mymensingh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Broiler

Objectives:

To replace commercial protein concentrate with SCP derived from autolyzed S. cerevisiae in broiler diet.

Methodology:

Production and autolysis of S. cerevisiae biomass- Molasses based media was used as the carbon source for mass production of S. cerevisiae. Sugarcane molasses was collected from the local market and 15% media was prepared with normal tap water and the Brix value was adjusted to 11°. The pH of the media was adjusted to 5.26. Commercial grade urea was added 6gL^-1 to increase nitrogen content of the media. 1.8L of previously prepared S. cerevisiae pure stock was inoculated to 350L growth medium following autoclave at 121°C and 15 PSI for 30 minutes and cooling. The fermentation process was carried out within a customized bioreactor at 28- 30°C for 72 hours. During the culturing process, 15 litre/minute purified air was supplied to the media to maintain aerobic condition. S. cerevisiae was harvested by gravitational method. Then the cells were washed with distilled water and autolyzed at 55°C for 36 hours. After autolysis, the autolyzed S. cerevisiae was kept at 80°C for 30 mins to stop the autolysis process as described by Tangüler and Erten (2009). Then the autolysate was dried at 60°C to remove moisture. Feeding trial- Oven dried autolyzed S. cerevisiae was used to replace the protein concentrate at 25%, 50% and 100% in feed. Experimental diets were formulated to meet up the nutrients requirement of ROSS-308 using high quality commonly available feed ingredients. The diets were formulated for starter and grower phases and presented to the birds in mash form. The chicks were randomly assigned to different dietary groups in a completely randomized design (CRD). The duration of the experiment was 35 days. Management of experimental broilers- Day old chicks were collected from local commercial hatchery and individually identified by wing banding and randomly assigned into four dietary groups; control group (0%), three replacement groups (25%, 50% and 100%) with 24 chicks per group. The experiment was conducted at the open sided house of Bangladesh Agricultural University Poultry Farm. 5 cm deep rice husk was used as litter material. One chick feeder and drinker were provided in each pen. The feeders and drinkers were fixed in such a way that the broilers were able to eat and drink conveniently. Drinkers were washed and cleaned everyday while feeder were cleaned once in a week. Feed and fresh drinking water was supplied ad-libitum basis. Birds were vaccinated against Infectious Bronchitis (IB), New castle Disease (ND) and Infectious Bursal Diseases (IBD). Standard biosecurity measures were followed throughout the rearing period. Data collection and record keeping- Weekly body weights of individual bird and weight of feed given and left over were measured and recorded. Live body weight (LBW), body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR) were calculated for each growth phases. At the end of the feeding trial, one broiler per replication having similar body weight was sacrificed and used for recording different carcass parameters. The birds were sacrificed and allowed to bleed completely and scalded by immersing in hot water (51-55°C) for 2 min. The feathers were removed by hand pinning. Finally, records were kept on weight of dressed broilers, feather, blood, shank, liver, heart, gizzard, spleen, head, neck and intestine. The contents of gizzard were expelled to record its weight. Statistical analysis- Collected data were analyzed using a linear model implemented in R (R Core Team, 2016). Effect of diets, individual and replication were treated as fixed effects. Diet effects on different body weight, growth and carcass parameters were compared with control diet by Dunnett’s test.

Source of Information: Bang. J. Anim. Sci. 2019. 48 (1): 1-8

Article Link (Online Journal): DOI: https://doi.org/10.3329/bjas.v48i1.44551

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The findings of this study suggest that autolyzed S. cerevisiae might be used to replace protein concentrate at a level of 25% and 50% in broiler diet without compromising growth and carcass quality. However, further study with female chicks might help to recommend more precise inclusion level of autolyzed S. cerevisiae as a source of SCP in the broiler diet.

Knowledge Management: Journal