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Fact Findings About the Frozen Buck Semen Characters Freezing with Eyc and Tris Extender and Productivity of Black Bengal Does as the Potential Genetic Resource in Bangladesh

Md. Fazlul Karim
Govt. Poultry Farm, Rangpur-5402, Bangladesh

M. A. M. Yahia Khandoker
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Syed Sakhawat Husain
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The research work was conducted at the Artificial Insemination Center under the Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh to compare the efficacy between Egg Yolk Citrate (EYC) and Tris diluter for frozen semen production in Black Bengal buck. The parameters of semen characteristics included volume per ejaculate (ml), percentage of individual motility (progressive), normal and live sperm and sperm concentration (billion/ml). After dilution with EYC extender, It revealed from statistical analysis of frozen semen that individual bucks had significant effect (p<0.05) on sperm motility and but insignificant on motility after cooling with and without glycerol. In the same way, with Tris diluter, it was insignificant (p>0.05) on diluted semen motility and motility after cooling with and without glycerol. Motility and morphology of the sperm after equilibration and thawing showed insignificant difference among the bucks using EYC diluter. On the contrary, variation in the motility after equilibration and thawing was found significant (p<0.05) using Tris diluter and insignificant on normal and live sperm percentages. After insemination with frozen buck semen, productivity or conception rate was found significantly (p<0.01) higher (60.37%) in Tris than that of EYC diluter (43.75%). On the other hand, the productivity when compared among bucks within the diluter, the variation was not found significant (p>0.05).The productivity found both in Tris and EYC diluter is almost similar to abundantly used Triladyl diluter (58.25). It is concluded that tris diluter might be used as the alternative to the Tryladil diluter though further study is to be needed for more confirmation.

Keywords: EYC, Black bengal buck, Genetic resource, Productivity, TRIS

Location: Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Animal

Objectives:

To find out the the Frozen Buck Semen Characters Freezing with Eyc and Tris Extender and Productivity of Black Bengal Does as the Potential Genetic Resource in Bangladesh.

Methodology:

The study was conducted at the Artificial Insemination (AI) Center under the Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh. A total of six adult Black Bengal bucks were selected from Nucleus breeding flock (NBF) based on body weight, libido, reaction time, scrotal circumference (SC), volume and also their ability to produce semen having greater than 80% morphologically normal spermatozoa with satisfactory motility, live spermatozoa and concentration. The age, body weight and scrotal circumference (SC) of bucks were 15 to 28 months, 19.0 to 25.0 kg and 17.0 to 22.0 cm respectively. The bucks were reared in individual pen (4×2.5 sq.ft) and they were fed with Napier, Ipil-Ipil and/or German grass twice daily as per requirement. The feed was supplemented with commercial concentrate in pellet form (crude protein content: 120g/kg DM and energy content: 10.4 MJ ME/kg DM) in the morning and again in the afternoon at the rate of 100 gm/ buck. They were allowed for grazing and exercise for 1 to 2 hours daily. The breeding bucks were also supplied with germinated gram (20gm/buck/day) and clean and safe water. Egg yolk-citrate diluter was used for the extension of semen. Before use, the egg yolk-citrate diluter was prepared according to Herman and Madden (1963). A stock solution for tris-glucose egg yolk diluents was prepared by dissolving tris-glucose and citrate in 85 ml distilled water. Collection of semen was done with artificial vagina maintaining optimum temperature about 41-43^oC. Semen was collected twice a week within 8.30 AM from each buck after cleaning the prepuce with antiseptic solution (Savlon). The individual ejaculate was evaluated immediately using the method by Herman and Madden (1963). One part of the splitted semen sample was diluted with egg yolk-citrate and another part with Tris diluter in a way so that 100 million motile spermatozoa per insemination dose were prevailed. Then the diluted semen was evaluated for motility (%), normal and abnormal sperm count (%), live and dead spermatozoa. For the production of frozen semen, semen samples were diluted with egg-yolk-citrate and tris diluter. After evaluation, the semen was diluted on the basis of sperm concentration per ejaculate with the egg yolk-citrate, Tris and Triladyl based cryodiluent (diluter + cryoprotectant) to obtain a final concentration of 100 million spermatozoa per insemination dose. The motility (%) of the diluted semen was observed and recorded. Extended semen was then filled manually into 0.50-ml straws and the laboratory ends of the straws were sealed with Polyvinyl chloride alcohol complexes (PVC) powder. After sealing and filling, the straws were placed in the refrigerator at 4-5°C for 3 hours for equilibration. The motility of the equilibrated sperm was checked and only samples with more than 60-70% motility were used for freezing. After cooling the diluted sperm was placed in controlled rate freezer and initiated freezing. Conception rate was recorded as the percentage of does that had not returned to estrus within 42 days (two cycles) after AI. This conception rate was also ensured by taking the history from the owner. Then conception rate was calculated by using the following formula: Conception rate (CR) = (Number of does conceived ) / (Number of does inseminated) x 100. The data generated from this experiment were entered in Microsoft Excel worksheet, organized and processed for further analysis. Analysis was performed with the help of Statistical Analysis System Computer Package (SAS, 1998).

Source of Information: Res. Agric. Livest. Fish. Vol. 5, No. 3, December 2018 : 341-350

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In case of fresh diluted semen with EYC diluter, individual bucks had significant effect (p<0.05) on diluted semen motility and but insignificant on motility after cooling with and without glycerol. On the other hand, it was insignificant (p>0.05) on diluted semen motility and motility after cooling with and without glycerol with Tris diluter. It revealed from statistical analysis that the motility after equilibration and live sperm percentages differed significantly (p<0.05) when EYC and Tris diluter were considered for frozen semen production. There was also significant variation (p<0.01) between EYC and Tris on motility after thawing. No significant variation was found on normal sperm percentages. Significant variations of semen parameters were observed between diluter to diluter and buck to buck. No significant variation was found on normal sperm percentages. The efficacy of tris extender is better than that of EYC diluter taken into considerations all semen attributes. The productivity with EYC was 43.75% but with frozen semen made with Tris was 55.90%. When the conception rate of semen diluted with EYC and Tris was considered separately, no significant difference (p>0.05) of conception rate by the bucks was observed. But when the conception rate was compared between the two kinds of frozen semen (diluted with EYC and Tris) it was revealed that the kind of semen had no significant effect (p>0.05) on conception rate. Considering the conception rate, it was reported that the highest conception rate was obtained by the semen of buck 30 and lowest by buck 3, though further study is to be needed for more economic confirmation.

Knowledge Management: Journal