Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Morpho-physiology and Anatomical Responses of Sorghum Seedlings as Affected by Salinity in Hydroponic Culture

M. N. Sarkar
Dept. of Crop Botany, Bangladesh Agricultural University, Mymensingh, Bangladesh

A. K. M. Z. Hossain
Dept. of Crop Botany, Bangladesh Agricultural University, Mymensingh, Bangladesh

S. N. Islam
Bangladesh Jute Research Institute, Monirumpur, Jessore, Bangladesh

S. Shahanaz
Department of Agricultural Extension, Monirumpur, Jessore, Bangladesh

M. Z. Tareq
Bangladesh Jute Research Institute, Jagir, Manikganj, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted at the growth chamber of Department of Crop Botany in the Bangladesh Agricultural University, Mymensingh, Bangladesh during March 2018 to observe morpho-physiology and anatomical response of sorghum. Six genotypes were grown in hydroponics with a full nutrient solution (NH₄NO₃-500 μM; Ca(NO₃)₂-500 μM; MgSO₄-200 μM; KH₂P0₄ -100 μM; FeC1₃-2μM; H₂B0₃-11 μM; MnC1₂-2μM; ZnC1₂-0.35μM; CuCl₂-0.2μM; (NH₄)₆Mo₇0₄-0.1 μM) and 100 mM salinity was imposed on 14 days seedlings. Data on morpho-physiological and anatomical parameters from seedlings were collected after 21 days and stress tolerant indexes of shoot and root were analyzed. Anatomical parameters like metaxylem and protoxylem thickness were also investigated. The results indicated that all the parameters viz. root length, shoot length, fresh and dry weight of shoot and root, stress tolerance index of root, and shoot, dry weight of shoot and root, relative chlorophyll content, photochemical efficiency (Fv/Fm), proline concentration, total root area, vascular cylinder area and root diameter were decreased with increasing salinity levels except leaf proline content. Genotypes BD 750 and BD 686 showed better performance considering tolerant indicators while the poor performance was exhibited by BD 747 and BD 753. Thus, based on overall observation BD 750 and BD 686 might be salt tolerant.

Keywords: Sorghum, Salinity, Hydroponic culture, Morpho-physiology, Anatomy

Location: Department of Crop Botany, Bangladesh Agricultural University, Mymensingh

Start Date: 00-00-2018

End Date: 00-00-2018

Program Area: Crop-Soil-Water Management

Non-commodity: Sorghum

Objectives:

The study was designed to investigate the morphophysiology and anatomical responses of sorghum seedlings to salinity in hydroponic culture.

Methodology:

Plant growth, environment, and treatments The experiment was conducted at the growth chamber of Department of Crop Botany in Bangladesh Agricultural University, Mymensingh, Bangladesh during March 2018. The experiment was conducted using six sorghum genotypes namely BD 686, BD 687, BD 693, BD 753, BD 750 and BD 747 to test their morpho-physiology and anatomical attributes against salinity (0 and 100 mM) at osmotic and ionic phases. The experiment was designed by using Completely Randomized Design (CRD) having three replications. Seeds were collected from Bangladesh Agricultural Research Institute (BARI), Gazipur. The composition of the full strength nutrient solution was prepared according to Pitann et al. (2009). Seeds were surface sterilized with 5% sodium hypochlorite for 30 min and washed three to four times with distilled water. Then the seeds were soaked overnight with water for imbibition. For salinization, NaCl was dissolved with nutrient solution to reach 100mM salinity. The salinity level was measured through EC using Sens ION EC-5 meter (Hach, Loveland, Colorado, US). At the age of 14 days of sorghum seedlings, saline treatments were imposed for next 8 days. Chlorophyll determination- An index of the relative leaf chlorophyll content was measured by a chlorophyll meter (SPAD- 502, Konica Minolta, Japan). Readings were taken along the middle of the four leaves of one plant and the mean value was used for analysis. The measurements were made on ten plants from each treatment. Chlorophyll fluorescence was measured with Pocket-PEA (Hansatech, Norfolk, UK). The maximal photo-chemical efficiency of PSII, Fv/Fm= (Fm− Fo)/Fm (Genty et al., 1989) was measured in dark-adapted (30 min) leaves at 8^th day. Well-developed 4^th leaves were selected for measurement. Proline determination-The proline content of the supernatant was determined according to the method described by Zhang and Huang (2013). In brief, for the determination of proline content 0.50 g fresh leaves were collected from the 120-day old sorghum plants. The leaves were homogenized on a mortal-pastel with 4.0 mL of 3% sulfosalicylic acid and centrifuged at 11,500 × g for 15 min. The supernatant was used for the proline estimation. Anatomical data collection-The stressed plant roots were collected 14 days after salinization. For root anatomical studies, 1 cm root segments were collected after discarding 2 cm from root tip. Both well-developed 4^th leaf and root segments were kept in ethanol till transverse sections were made to observe the differences in anatomical structure. Root and leaf anatomy of stressed and controlled plants were observed by light microscope (Axioscop; Carl Zeiss, Oberkochen, Germany). Here 10x and 40x of images were observed and captured. Images were processed for taking various anatomical parameters using the ZEISS ZEN digital imaging software for light microscopy. For root, metaxylem thickness and protoxylem thickness were measured from the same xylem strand. Vascular cylinder area and total root diameter were also measured. In each cell three positions were selected to take thickness values and then the average was calculated. Statistical analysis-The collected data were subjected to statistical analysis following ANOVA technique. Differences among treatment means were adjusted by Duncan’s Multiple Range Test with the help of a computer based statistical package program MSTAT-C (Gomez and Gomez, 1984).

Source of Information: The Agriculturists 2019; 17(1-2) 52-65

Article Link (Online Journal): DOI: https://doi.org/10.3329/agric.v17i1-2.44696

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It could be concluded based on relative value at hydroponic condition, BD 759 and BD 686 can be expressed as tolerant and BD 747 and BD 753 as susceptible genotypes to salt stress. Moreover, further study is needed to evaluate the genotypes in the field condition, especially in the coastal areas in Bangladesh for their adaptability to grow.

Knowledge Management: Journal