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Effects of Shoti (curcuma Zedoaria) on Growth Performances and Hemato-biochemical Parameters of Broiler Chicks

M. J. Alam
Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. S. Islam
Department of Physiology & Pharmacology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh

M. M. Rahman
Department of Physiology & Pharmacology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh

S. Mazumder
Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

N. Ahmad
Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The research was conducted to observe the effect of shoti meal on weight gain and some selected hematological values such as total erythrocyte count (TEC), hemoglobin (Hb) and erythrocyte sedimentation rate (ESR) and biochemical parameters such as aspartate transaminase (AST), alanine transaminase (ALT), uric acid and creatinine in broilers. Fresh rhizomes were washed, minced and dried and pulverized in a flour meal. A total of 20 broilers (16 day old) were randomly divided into four equal groups. Group A was treated as control and groups B, C and D were fed with 5%, 10% and 15% shoti respectively for 22 days. The result showed that body weight of broilers fed 10% shoti meal (group C) was higher than that of other treated groups. After having completed the feeding trial, birds were sacrificed to collect blood samples for hematological and serum biochemical analysis. Total erythrocyte count (TEC) in group D varied significantly (p<0.05) from all other groups. The highest hemoglobin content was recorded in group B and lowest in group A (Control). Significant (p<0.05) differences were observed among the groups compared to control group. The highest erythrocyte sedimentation rate (ESR) was in group B and lowest was in group A (control) and group C (shoti 10%). Serum AST within the treated groups 15% shoti, (Group D) was significantly (p<0.05) higher to that of 5% shoti (Group B), and 10% shoti (group C). The ALT value in group A was significantly higher (p<0.05) than the other groups. Uric acid concentration within the treated groups, group C (10% shoti) showed significantly higher (p< 0.05) than the group B (5% shoti) and group D (15% shoti). Creatinine concentration decreased significantly (P<0.05) in control (group A) to that of others group. Biochemical parameters analyzed in present research is the first report in broilers.

Keywords: Shoti, Shoti, Broiler hemato-biochemical, Broiler, hematology

Location: Department of Physiology of Bangladesh Agricultural University (BAU), Mymensingh

Start Date:

End Date:

Program Area: Animal Health and Management

Non-commodity: Performance

Objectives:

To study the effects of shoti based diet on growth performance, hematology and some serum biochemical parameters in broiler chicks.

Methodology:

Experimental design- The experiment was conducted in the Department of Physiology of Bangladesh Agricultural University (BAU), Mymensingh, where birds were reared in the poultry rearing facilities of the Department in an ambient environment. Twenty Cobb-500 broilers broiler chicks aging 16 days were collected from local commercial broiler farm and randomly divided into four equal groups. Group A was considered as control and groups B, C and D were considered as treated groups. Shoti meal was prepared at 5%, 10% and 15% fed to group B, C and D respectively with feed throughout the experimental period. Body weight of individual bird was recorded at the beginning and at every three day up to the end of experiment for 35 days. The birds were sacrificed to collect blood sample for hematological and biochemical analysis. Shoti meal preparation- Curcuma zedoaria (shoti) was collected from BAU campus and sorted on the basis of white color of the rhizome. Then they were washed, chopped into pieces, sun dried and pulverized in a flour meal. The procedure followed was essentially as that was Latif et al. (1979). Management of the chiks- The commercial management procedures were followed during the whole experimental period. Fresh and dried rice husk was used as a litter at a depth of 2 cm. The litter was stirred three times in a week to prevent cake formation. Once in the whole experimental period old litter material was changed using new rice husk to prevent fungal or coccidial infection. Each pen was 2.5 ft x 2 ft and was allocated for five birds. Therefore, the space given for each bird was 1 square ft. The birds were always exposed to continuous lighting of 12 hours a day. Electric bulbs were used to provide necessary light. In order to maintain required temperature (105⁰F) and humidity inside the pens, all the windows of the laboratory were kept open during day and during night a 100 watt bulb was provided as a source of heat. One trough feeder and one round waterer were provided for each pen. Feeders were cleaned everyday at morning and afternoon and fresh clean drinking water was supplied for all the times. Proper hygienic and sanitation programs like foot bath of the shed, not to the entrance of wild bird to the shed, apron and gloves use during feeding of the bird etc. were followed during the experimental period. Measurement of body weight- The body of each birds was measured with the help of electric balance on the day 16 of age (0 day of experiment) and subsequently at every third days interval up to slaughter. Total weight gain in % = Average final weight - Average initial weight/Average initial weight. Collection of blood- Blood from each bird was collected at slaughter. A number of sterile test tubes containing anticoagulant (3.8% Trisodium citrate solution) at a ratio of 1:10 were taken. About 5.0 ml of blood was collected for hematological studies. The hematological studies were performed within two hours of collection. Preparation of serum- Three ml of blood was collected from wing veins in the sterile glass test tube. The blood containing tubes were placed in slanting position at room temperature for clotting. Then the tubes were incubated overnight in refrigerator (4⁰C) and the serum was collected. The sample was centrifuged at 1000 rpm for 15 minutes to have a more clear serum. The serum samples were separated and stored at -20⁰C till analysis. The erythrocyte sedimentation rate (ESR) was determined in a period of one hour using Wintrobe hematocrit tube. The result was expressed in mm/in 1^st hour. The hemoglobin content and packed cell volume were determined as per method by Lamberg and Rothstein (1977). The result was expressed in gm percentage (gm %) and percentage (%) respectively. AST and ALT measurement was performed using automatic blood analyzer (Stat Fax 3300, Drawen 1679, and Palm City, FL 34991, USA). The result was expressed in U/L. Colorimetric reaction (Jaffe reaction) of creatinine with alkanine picrate measured kinetically at 490 nm (490-510), without any pretreatment step. This reaction has been improved (specificity, speed, and adaptability) by the development of an initial rate method. Colorimetric reaction of NH₃ with salicylate and hypochlorite measured kinetically at 580 nm, without any pretreatment step. This reaction has been improved (specificity, speed and adaptability) by the development of an initial rate method. Statistical analysis- A randomized complete block (RCB) design with more than one observation per cell was applied. The data were collected and the Mean ± SE were calculated by using descriptive statistics. An analysis of variance (ANOVA) table had been constructed with the help of computer package MSTAT for identifying any statistically significant difference among the groups. The mean difference among the treatments was determined as per Duncan's multiple range test (Gomez and Gomez, 1984).

Source of Information: Bangl. J. Vet. Med. (2016). 14 (1): 15-19

Article Link (Online Journal): DOI: https://doi.org/10.3329/bjvm.v14i1.28817

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The highest erythrocyte sedimentation rate (ESR) was in group B and lowest was in group A (control) and group C (shoti 10%). Serum AST within the treated groups 15% shoti, (Group D) was significantly (p<0.05) higher to that of 5% shoti (Group B), and 10% shoti (group C). The ALT value in group A was significantly higher (p<0.05) than the other groups. Uric acid concentration within the treated groups, group C (10% shoti) showed significantly higher (p< 0.05) than the group B (5% shoti) and group D (15% shoti). Creatinine concentration decreased significantly (P<0.05) in control (group A) to that of others group. Biochemical parameters analyzed in present research is the first report in broilers.

Knowledge Management: Journal