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Comparative Efficacy of Two Extenders on Post-thaw Sperm Characteristics of Cryopreserved Black Bengal Buck Semen

Md. Fazlul Karim
Upazilla Livestock Officer, Sundorganj, Gaibandha-5700

M. A. M. Yahia Khandaker
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Syed Sakhawat Husain
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The research work was conducted at the Artificial Insemination (AI) Center under the Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh to compare the efficacy between Egg Yolk Citrate (EYC) and Tris extenders for cryopre served semen production in Black Bengal buck. The parameters of semen characteristics included percentage of individual motility (progressive), normal and live sperm and sperm concentration (billion/ml). Statistical analysis of fresh semen of individual buck effect was found to be significant (p<0.01) on normal sperm percentage but insignificant on individual motility, sperm concentration and live sperm percentage. After dilution with EYC extender, individual bucks had significant effect (p<0.05) on sperm motility and but insignificant on motility after cooling with and without glycerol. In the same way with Tris extender, it was insignificant (p>0.05) on diluted semen motility and motility after cooling with and without glycerol. Motility and morphology of the sperm after equilibration and thawing showed insignificant difference among the bucks using EYC extender. On the other hand, variation in the motility after equilibration and thawing was found significant (p<0.05) using Tris extender and insignificant on normal and live sperm percentages.

Keywords: Comparative efficacy, Motility, EYC, TRIS, Black Bengal buck

Location: Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Deer

Objectives:

To test the efficacy of different extender in the preservation of buck semen.

Methodology:

The study was conducted at the Artificial Insemination (AI) Center under the Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh. A total of six adult Black Bengal bucks were selected from Nucleus breeding flock (NBF) based on body weight, libido, reaction time, scrotal circumference (SC), volume and also their ability to produce semen having greater than 80% morphologically normal spermatozoa with satisfactory motility, live spermatozoa and concentration. The age, body weight and scrotal circumference (SC) of bucks were 15 to 28 months, 19.0 to 25.0 kg and 17.0 to 22.0 cm respectively. The bucks were reared in individual pen (4×2.5 sq.ft) and they were fed with Napier, Ipil-Ipil and/or German grass twice daily as per requirement. The feed was supplemented with commercial concentrate in pellet form (crude protein content: 120g kg^-1 (DM) and energy content: 10.4 MJ ME kg^-1 (DM) in the morning and again in the afternoon at the rate of 100 g buck^-1. They were allowed for grazing and exercise for 1 to 2 hours daily. Clean and safe water was also made available at all the time. The breeding bucks were also supplied with germinated gram (20g buck^-1 day^-1). Egg yolk-citrate extender was used for the extension of semen. Before use, the egg yolk-citrate extender was prepared according to Herman and Madden (1963). A stock solution for tris-glucose egg yolk diluents was prepared by dissolving tris-glucose and citrate in 85 ml distilled water. Collection of semen was done with artificial vagina maintaining optimum temperature about 41-43^oC. After complete mounting over the teaser male (with seeking movement of the penis), the artificial vagina was touched with the glans penis. Semen was collected twice a week within 8.30 AM from each buck after cleaning the prepuce with antiseptic solution (Savlon). The individual ejaculate was evaluated immediately after collection using the method described by Herman and Madden (1963). One part of the splitted semen sample was diluted with egg yolk-citrate and another part with Tris diluter in a way so that 100 million motile spermatozoa per insemination dose were prevailed. Then the diluted semen was evaluated for motility (%), normal and abnormal sperm count (%), live and dead. The data generated from this experiment were entered in Microsoft Excel worksheet, organized and processed for further analysis. Analysis was performed with the help of Statistical Analysis System Computer Package (SAS, 1998, version 6.03. SAS Institute Inc. Cary NC, 25-109 USA).

Source of Information: Res. Agric. Livest. Fish. Vol. 6, No. 1, April 2019 : 119-125.

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In case of diluted semen with EYC extender, individual bucks had significant effect (p<0.05) on diluted semen motility and but insignificant on motility after cooling with and without glycerol. On the other hand, it was insignificant (p>0.05) on diluted semen motility and motility after cooling with and without glycerol with Tris extender. It revealed from statistical analysis that the motility after equilibration and live sperm percentages differed significantly (p<0.05) when EYC and Tris diluter were considered for frozen semen production. There was also significant variation (p<0.01) between EYC and Tris on motility after thawing. No significant variation was found on normal sperm percentages. Significant variations of semen parameters were observed between extender to extender and buck to buck. No significant variation was found on normal sperm percentages. The efficacy of tris extender is better than that of EYC extender taken into considerations all semen attributes though further study is to be needed for more economic confirmations.

Knowledge Management: Journal