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High frequency in vitro regeneration of gynura Procumbens (lour.) merr.

Tanjina Akhtar Banu
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

Barna Goswami
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

Shahina Akter
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

Mousona Islam
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

Tammana Tanjin
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

Ahashan Habib
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

Salim Khan
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

Abstract/ Executive Summary:

An efficient rapid in vitro regeneration protocol was described from nodal segment, leaf and petiole explants. MS medium supplemented with 1.0 mg/l BAP and 0.5 mg/l IAA was found best for the multiple shoot formation from nodal segments. In this combination 99% explants produced multiple shoots and the average number of shoots per explants was 20.1 ± 1.96. For petiole and leaf explants best response was observed on MS supplemented with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. Petiole explants produced highest mean number of shoots/explant (22.9 ± 1.728) among the three explants when the explants were cultured on MS with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. The highest frequency of root induction (100%) and mean number of roots/plantlets (11.75) were obtained on MS. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.

Keywords: Gynura procumbens, High frequency, Regeneration

Location: BCSIR research field, Bangladesh

Start Date:

End Date:

Program Area: Farm Mechanization

Commodity: Medicinal Plants

Objectives:

To establish a reproducible in vitro regeneration protocol using different type of explants (nodal, leaf and petiole) for large?scale production of G. procumbens followed by conserving biodiversity and commercial propagation of medicinally important plants Gynura in outdoor field condition.

Methodology:

Three types of explants (Node, young leaf and petiole segments) were used for the present experiment. Explants were collected from field grown plants of BCSIR research field. Surface sterilization procedure of explants was followed according to the protocol described by Khan et al. (2016). Sterilized explants were inoculated and cultured on MS containing BAP, NAA, IAA, Kn, 2,4-D and IBA singly or in combinations for direct and indirect (through callus) in vitro regeneration of shoots. In vitro regenerated shoots were sub-cultured regularly to fresh medium at an interval of 21- 28 days for further multiplication. Elongated shoots were separated and cultured on rooting medium for root formation. About 1 - 3 cm long shoots were separated and cultured on rooting medium containing MS and half strengths of MS alone or with combination of IBA. All in vitro grown cultures were maintained under illumination on a 16 hrs photoperiod at 25 ± 2ºC. The well rooted plantlets were then kept in room temperature for 2 - 3 days and transferred to plastic pot containing garden soil and compost in ratio of 2 : 1 and moist them adequately for proper hardening.

Source of Information: Plant Tissue Cult. & Biotech. 27(2): 207?216, 2017 (December).

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It can be concluded that, the micropropagation protocol reported in the present study was characterized with a rapid proliferation of shoots using various explants (node, leaf and petiole), easy rooting of the micro?shoots and the plantlets were easily acclimatized to the external environment and undergoing normal physiological development. Moreover, there was no report available for the micropropagation of Gynura procumbens using leaf and petiole segments. So, this regeneration protocol is highly advantageous for conservation, large scale production as well as uniform source of Gynura procumbens plants to yield important secondary metabolites for pharmaceutical industries with a range of further biotechnological applications.

Knowledge Management: Journal