Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Shoot Multiplication of Gerbera Callus Grown from Flower Buds Under Different Growth Regulators Composition

S. Parvin
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh

N. Sabrin
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh

M. R. Jahan
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh

H. Ahmad
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh

A. F. M. Jamal Uddin
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted in at Advanced Seed Research and Biotech Center (ASRBC), ACI Limited;Dhaka, Bangladesh during the period of September to October, 2014 to evaluate different growth regulator composition towards shooting from callus produced using the flower bud of selected mother plant of gerbera. Callus grown from flower buds were subjected to six growth regulator composition viz.T0: 0 mg/l BAP + 0 mg/l NAA, T1: 1 mg/l BAP + 0.01 mg/l NAA, T2:2 mg/l BAP + 0.01 mg/l NAA, T3: 3 mg/l BAP + 0.01 mg/l NAA, T4:4 mg/l BAP + 0.01 mg/l NAA, T5:5 mg/l BAP + 0.01 mg/l NAA. Number of responsive explants (34.3), Percent (%) of responsive explants (85.8%), Number of days taken for shoot initiation (16 days), Number of shoots per clump (8.0), Number of leaves per shoot (7.70), Shoot length (25.4 mm) were found best in T3 whereas the lowest was observed from T1 and T5. Thus, combination of 3 mg/l BAP + 0.01 mg/l NAA can be considered as the best composition of growth regulators for shoot multiplication of gerbera callus grown from flower buds.

Keywords: Shoot multiplication, BAP, NAA, Protocol development

Location: At Advanced Seed Research and Biotech Center (ASRBC), ACI

Start Date: 00-09-2014

End Date: 00-10-2014

Program Area: Variety and Species

Commodity: Gerbera

Objectives:

To find out the best growth regulator composition for shoot multiplication of calluses grown from flower bud.

Methodology:

The experiment was carried out at Advanced Seed Research and Biotech Center (ASRBC), ACI during the period from September to October 2014 to evaluate different growth regulator concentration towards shooting from callus produced using the flower bud of selected mother plant of gerbera. It has been already found from a previous experiment that flower bud is the most promising explant for Gerbera in producing callus (Parvin et al. 2017). The present experiment is going to be the next step in development of the standard tissue culture protocol of gerbera for Bangladesh. A gerbera cultivar producing double flower with magenta ray floret and black yellow disc floret was selected as mother plant. Immature inflorescences of 0.5 to 0.7 cm in diameter were collected from the plants. After sterilization, the buds were cut into two halves. The explant washed under running tap water with few drops of Tween twenty for 15 –20 minutes. Then they were immersed in 70% ethanol for 1 minute and washed thoroughly with distilled water. They were later immersed in 0.1 per cent HgCl2 for 7 minutes and finally washed with double glass distilled water for 3-4 times in laminar air flow cabinet to remove any traces of HgCl2. Each explant was inoculated separately on solid medium and temperature of the culture room was maintained within 25±1ºC by an air conditioner and 16 hour photoperiod was maintained along with light intensity of 3000 lux for proper growth and development of culture. The developing cultures are then subjected to the following growth regulator concentrations to screen out the best one for shoot multiplication. Growth regulators:T0: 0 mg/l BAP + 0 mg/l NAA, T1: 1 mg/l BAP + 0.01 mg/l NAA, T2: 2 mg/l BAP + 0.01 mg/l NAA, T3: 3 mg/l BAP + 0.01 mg/l NAA, T4: 4 mg/l BAP + 0.01 mg/l NAA, T5: 5 mg/l BAP + 0.01 mg/l NAA. Data on number of responsive explants, % of responsive explants, number of days taken for shoot initiation, number of shoots per clump, number of leaves per shoot and shoot length (mm) were collected and were statistically analyzed using MSTAT-C computer package program. Mean for every treatments were calculated and analysis of variance for each one of characters was performed by F–test (Variance Ratio). Difference between treatments was assessed by Least Significant Difference (LSD) test at 5% level of significance (Gomez and Gomez, 1984).

Source of Information: J. Biosci. Agric. Res. 17(02): 1442-1447

Article Link (Online Journal): https://doi.org/10.18801/jbar.170218.179

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The findings of the present experiment shows that the combination of 3 mg/l BAP + 0.01 mg /l NAA performs best in case of shoot multiplication from the proliferating clumps of gerbera callus grown from flower buds. Rooting performance of these will be the next endeavor in formulating the protocol.

Knowledge Management: Journal