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Effect of Dietary Vitamin C on the Growth and Survival Rate of Rohu (labeo Rohita)

Meraz Ahmed
Dept. of Aquaculture, Bangladesh Agricultural University, Mymensingh

Zannatul Ferdous
Dept. of Aquaculture, Bangladesh Agricultural University, Mymensingh

Mritunjoy Paul
Freshwater Station, Bangladesh Fisheries Research Institute, Mymensingh

Md. Harunor Rashid
Freshwater Station, Bangladesh Fisheries Research Institute, Mymensingh

Md. Sazzad Hossain
Dept. of Aquaculture, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

The experiment was carried out to evaluate the effect of dietary vitamin C on the growth and survival rate of rohu (Labeo rohita) for a period of 49 days from 5^th April to 24^th May, 2016 in 12 experimental aquaria in Wet Laboratory of the Department of Aquaculture, Bangladesh Agricultural University, Mymensingh. Fingerlings of initial weight of 6.02±0.00 g was released at the same stocking density (12 fingerlings per aquaria). Four different treatments (T1, T2, T3 and T4) each with three replications were used having different amount of vitamin C, T1 (0 mg vitamin C/kg feed), T2(200 mg vitamin C/kg feed), T3 (400 mg vitamin C/kg feed) and T4 (600 mg vitamin C/kg feed). All four diets having a constant inclusion level of the following ingredients: fish meal 40%, rice bran 25%, wheat bran 25%, molasses 5%, soybean oil 4% and vitamin and mineral premix 1%. Final weight (g), weight gain (g), percent weight gain (%), specific growth rate (%/day), and protein efficiency ratio, varied from 7.31±0.14 to 7.83±0.15, 1.29±0.14 to 1.8±0.15, 21.48±2.37 to 30.12±2.54, 0.52±0.22 to 1.21±0.17 and 0.46±0.42 to 0.60±0.04, respectively. Highest FCR (7.81±0.22) was found in T1 and the lowest FCR (5.78±0.36) was found in T4. The highest PER (0.60±0.04) was found in T4 and the lowest PER (0.46±0.42) was found in T1. The highest survival rate (63.89±4.81) was found in T4 and lowest survival rate (47.22±9.62) was found in T1. Best growth performance was found in T4 followed by T3, T2 and T1. The present research findings suggested that the best growth performance and survival rate of Labeo rohita was obtained from supplementation of 600 mg vitamin C per kg feed containing diet which could be chosen by the fish feed manufacturer and fish farmers.

Keywords: Vitamin C, Labeo rohita, Growth, Survivality.

Location: Wet Laboratory, adjacent to the Faculty of Fisheries, Bangladesh Agricultural University (BAU), Mymensingh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Rohu

Objectives:

The present study was designed to evaluate the growth performances of rohu by feeding the feed formulated containing vitamin C which would be helpful the rural people in uplifting their nutrition, livelihood and additional income.

Methodology:

Experimental site: The proposed research work were undertaken in 12 glass aquaria (each contains 60L) at the Wet Laboratory, adjacent to the Faculty of Fisheries, Bangladesh Agricultural University (BAU), Mymensingh. Stored ground water was used for rearing the fish. Aeration was provided from a 1.0-HP compressor to all the experimental tanks. Experimental Design: The experiment was conducted in 12 glass aquaria considering four treatments [Treatment 1 (T₁), Treatment 2 (T₂) and Treatment 3 (T₃), Treatment 4 (T4),] each having three replications. In this experiment Diet 1: 0 mg vitamin C, Diet 2:200 mg vitamin C; Diet 3:400 mg vitamin C, Diet 4:600 mg vitamin C were used for the treatment 1, 2, 3 and 4, respectively. The Labeo rohitafry was randomly distributed at a rate of 12 fish per aquaria. All the aquaria were kept on 1 m high wooden table to facilitate better observation and accessibility. Sample collection and acclimation: Fingerlings of Labeorohita were collected from the fish seed production hatchery,adjacent area of Mymensingh district. Rohu fry hatched from single egg mass were used in this experiment and acclimated to laboratory condition in two glass aquarium (120 L capacity each) for 2 weeks by feeding a commercial carp diet. Feed ingredients collection and feed formulation: The selected ingredients for this experiment-fish meal, rice bran, wheat bran, molasses, Vitamin C; mineral and vitamin premix were purchased from local market. Four graded levels of vitamin C (L-ascorbic acid, AA) at 0, 200, 400, and 600 mg/Kg diets were included in the basal diet (AA will be supplemented separately to the basal diet at the expense of wheat flour). The ingredients were grinded, milled, weighed, mixed and pelleted with meat mincer through a 0.8 mm diameter. After pelleting, the feeds were air dried and put in an air-tight container. All diets were stored at -20°C until fed. experiment procedure: The experiment was conducted for 70 days. Each treatment had three replications. The fingerlings of Labeorohita initial were of 6.02 gm weight. The fingerlings were randomly distributed at a rate of 12 fish per aquaria. Feeding was done twice daily at 9.00 am and 5.00 pm. Partial change of water from each aquarium was done daily during the removal of feces and uneaten feed. Feeding rates: Fingerlings were fed with experimental diets twice daily in the morning at 9.00 am and in the afternoon at 5.00 pm throughout the study period. Fingerlings in each aquarium were fed daily at the rate of 10% of their body weight; the amount was fixed after observing that it was not interested to take more than that amount of feed.Sampling procedure: Initial and final weight of fish in each aquarium was recorded. Fish were bulk weighed at every seven (7) days interval to keep record of fish weight. Fish were netted by using a fine mesh scoop net and excess water was then removed from fish body gently by using a blotting paper before weighing to the digital balance. After weighing of the fingerlings, those were released in the aquarium.Analytical methods: Proximate composition of prepared feeds and individual ingredients were analyzed in the Nutrition Laboratory of the Department of Aquaculture, BAU following Association of Official Analytical Chemists (AOAC, 2000) methods. Data analysis: The collected data were statistically analyzed by one way ANOVA with the help of SPSS to see whether the influence of different treatments on these parameters were significant or not. The means of different treatment were compared by Duncan’s New Multiple Range Test (Duncan, 1955) to test the significance of variation between the treatment means. Standard error (SE) of the treatment means was calculated from the residual mean square in the analysis of variance. All statistical analyses were carried out by MS Excel 2000 (version 7.0).

Source of Information: J. Biosci. Agric. Res. 16(02): 1375-1385

Article Link (Online Journal): https://doi.org/10.18801/jbar.160218.170

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The experiment on “Effect of dietary vitamin C on the growth and survival rate of rohu (Labeorohita)” fingerlings reared in aquarium was conducted in which feeding was done twice a day with different vitamin C levels. The best growth rate was obtained at supplementation of 600 mg vitamin C per kg feed which was the highest in vitamin C level content. The experiment revealed that the best growth performance and survival rate of rohu (Labeorohita) fingerlings were obtained with supplementation of 600 mg vitamin C per kg feed. Further research may be conducted by increasing vitamin C levels than supplementation of 600 mg vitamin C/kg feed to observe the growth and survival of rohu fingerlings to find out the optimum level of vitamin C per kg feed in future which could be chosen by the fish farmers and feed manufacturers.

Knowledge Management: Journal