Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Biochemical Investigation and Seedling Mortality Rate of Some Selected Boro Rice Cultivars at Low Temperature Stress

BasuniaS. C.
Dept.of Agricultural Chemistry, Hajee Mohammad Danesh Science & Technology University, Dinajpur

Sarker, B. C.
Dept.of Agricultural Chemistry, Hajee Mohammad Danesh Science & Technology University, Dinajpur

Md. Omar Kayess
Dept.of Genetics and Plant Breeding, Hajee Mohammad Danesh Science & Technology University, Dinajpur

Md. Imanur Rahman
Dept.of Genetics and Plant Breeding, Hajee Mohammad Danesh Science & Technology University, Dinajpur

Md. Kajal
Dept.of Agroforestry and Environment, Hajee Mohammad Danesh Science & Technology University, Dinajpur, Bangladesh

Md. Rakibul Alam
Dept.of Genetics and Plant Breeding, Hajee Mohammad Danesh Science & Technology University, Dinajpur

Abstract/ Executive Summary:

A field study was conducted for biochemical investigation and seedling mortality rate determination of some selected Boro rice seedlings during the period of December, 2014 to January, 2015. Experiment time was characterized by prevailing low environmental temperature of below 15^oC. Eight rice cultivars (V1: Pariza; V2: BRRI dhan29; V3: Shaita Boro; V4: BRRI dhan 28; V5: Jotapari; V6: BRRI dhan 16; V7: Pashushail; V8: HybridSL-8H) of different genetic constituents were evaluated in randomized complete block design with four replications. Two temperature regime was applied for seedling culture. Ambient temperature (T0) for field investigation and 4^oC temperature (T1) environment was maintained only for physiological parameters. Changes of leaf Proline content, chlorophyll content (a and b), total carotenoid content and mortality rate were investigated. Result showed that V8 (Hybrid SL-8H) and V2 (BRRI dhan 29) seedling synthesized the maximum leaf Proline at low temperature than other cultivars. Highest amount of chlorophyll-a, was in V8T0seedlings (10.03 mg g^-1), while chlorophyll-b (2.063 mg g-1) and total carotenoid (4.0470 mg g^-1) were highest in V3T0. Maximum chlorophyll-a/b ratio (6.729 mg) was recorded in V7T0. Mortality rate was lowest in Hybrid SL-8H. Results of the study revealed that Hybrid SL-8H cultivar showed comparatively better potentiality to survive at low temperature that might be selected for cultivation and popularization in the low temperature region of Bangladesh.

Keywords: Boro rice, Low temperature, Bio chemicals, Mortality, Selection

Location: Research field of the department of Agricultural Chemistry, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh

Start Date: 00-12-2014

End Date: 00-01-2015

Program Area: Risk Management in Agriculture

Commodity: Rice

Objectives:

The present research was carried out to screen suitable cultivars that can be utilized in future breeding programs for the development of cold tolerant Boro rice (Winter rice) varieties.

Methodology:

Plant material: Eighthy brid as we as local rice cultivars were used in this investigation. Seeds of these selected rice cultivars were collected from Bangladesh Agricultural Development Cooperation (BADC). Experimental site, design and seedling: The research work was conducted at the research field of the department of Agricultural Chemistry, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh. The site is located in 25.13^oN latitude and 88.23^oE longitude and at an elevation of 34.5 m above the mean sea level. This site belongs to AEZ-1 (Agro-ecological Zone-1) (UNDP and FAO, 1988). All of eight cultivars are raised in small plots laid out on randomized complete block design with four replications. The whole area was divided into three blocks and each block was divided into five units maintaining plot dimension to 2×2 m². Plotto plot distance was 30 cm. Recommended rice production practice of Bangladesh Agricultural Research Council (BARC) was followed (BARC, 2005). Experimental site is situated in the tropical climatic zone, characterized by heavy rainfall during the months from May to September and minimum rainfall during winter season. Soil of the experimental field was sandy loam in texture with medium high land having soil with soil pH 6.0. The seeds of selected eight rice cultivars were sown in previously prepared seedbed. The sprouted seeds were placed in individual plots without any biasness at the rate of 330 g per plot. Proper irrigation was made as and when needed and weeding was done at 20 and 27 days after sowing (DAS). Proline content of rice seedling: Fresh leaf samples from rice seedlings at different times were used for the determination of Proline. Free Proline content was estimated using the acid Ninhydrin method (Bates, 1973).About 40 to 50 mg of fresh leaf sample by weight was collected in an eppendorf tube containing 0.5 ml 3% sulfosalicylic acid and homogenized well using eppendorfpastle. It was then placed on a vortex mixer for about 10 minutes. Then 0.5 mL of 3% sulfosalicylic acid was added in it again. The eppendorf tube was then centrifuged for 20 minutes at 25^oC temperature with 15000 rpm. Then the supernatant was collected in a test tube carefully with the help of micropipette. Then 1.0 mL of 3% sulfosalicylic acid was added again to the eppendorf tube and centrifuged for 20 minutes at 25^oC temperature with 15000 rpm followed by mixing well using vortex mixer for 10 minutes. The supernatant was collected and added to the previously collected supernatant. Acid ninhydrin solution was made by adding 1.25 g ninhydrin with 30 mL glacial acetic acid and 20 mL of 6 M phosphoric acid and warmed it until it dissolved. Standard proline solution was also prepared by adding 0, 1, 5, 20, 50, 100, 150, 200 and 300 μg per 2 mL of 3% sulfosalicylic acid in test tubes for preparing standard curve. Then 2 mL each of glacial acetic acid and acid ninhydrin solution were added to the test tubes containing sample and standard Proline solution. Test tubes were then heated for 15 minutes in dry block heater maintaining 96-100^oC temperature and the reaction was terminated in an ice bath. Optical densities of the solutions (sample and standard solution) were measured at 520 nm wave length using UV-visible spectrophotometer. Amount of Proline was determined from a standard curve. Chlorophyll content of rice seedling: Chlorophyll content of rice seedling was determined by the method described by (Arnon, 1949). On the other hand, total chlorophyll was determined using the formulae given by (Porra, 2002). Concentration of chlorophyll-a (Chl-a), chlorophyll-b (Chl-b), total chlorophyll and total carotenoid was measured by using formula.

Mortality rate: Germination percentage was determined at field level at the initial stage of the experiment. This was done by counting the number of germinated seed and total number of seed within a marked area. This counting was done after 10 days of first germination (DAG). The percent germination was first calculated using following formula.

Mortality rate =100- {(Number of germinated seeds/Total number of seeds) x 100}

Source of Information: J. Biosci. Agric. Res. 12(01): 976-985

Article Link (Online Journal): DOI: 10.18801/jbar.120117.120

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Hybrid varieties are more tolerant to low temperature stress as they synthesized more biochemical related to stress and also may be an important gene donors for breeding and genetic studies. Therefore, considering the above facts the Hybrid dhan SLY-8H is suitable for cultivation in Boro (winter) season.Further studies are suggested in different locations and agro-climate.

Knowledge Management: Journal