Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Screening of Salinity Tolerance of Rice at Early Seedling Stage

Sejuti Mondal
Dept. of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur 1706

Teresita H. Borromeo
Crop Science Cluster, College of Agriculture, University of the Philippines Los Banos (UPLB), College, Laguna, Philippines

Abstract/ Executive Summary:

The study was conducted at International Rice Research Institute (IRRI) to assess the response of dry season hybrid seeds including parental checks FL478 and NSIC Rc222 to salt tolerance (12 dS/m) at the seedling stage of rice using IRRI screening techniques. The total number of seeds were two hundred thirty-one and irrigated, flood prone, heat tolerance, salinity and problem soils, aerobic and anaerobic germination, rainfed lowland and South Asian samples were used for this experiment. Among them, only 1.73% populations (4 irrigated) were identified as tolerant, 18.18% moderately tolerant, 37.26% sensitive and 46.86% were highly sensitive. Moderately tolerant plants were found from irrigated, flood prone, salinity and problem soils, aerobic germination, anaerobic germination and rainfed lowland and South Asian genotypes. As there was Brown Plant Hopper (BPH) infestation in this experiment, the tolerant genotypes may also be tolerant to BPH and for that reason results were distorted for other genotypes. Therefore, study should be conducted under controlled environment to ascertain the level of salt tolerance of the moderately tolerant populations. Besides, the identified tolerant genotypes (4) should be further tested with 18 dS/m to determine their supremacy to salt tolerance at the seedling and reproductive stages and QTL analysis could be performed to determine the effects of each genomic region of the trait of interest.

Keywords: Salinity tolerance, Seedling stage, IRRI, FL478, NSIC Rc222

Location: Phytotron (glass house) and Green House (NG 02) of the International Rice Research Institute (IRRI), Philippines

Start Date: 00-12-2013

End Date: 00-01-2014

Program Area: Risk Management in Agriculture

Commodity: Rice

Objectives:

The objective of this study was to identify the tolerant and sensitive genotypes from DS hybrid seed samples (231 seeds) which included irrigated, flood prone, anaerobic germination, salinity and problem soils, aerobic, rainfed lowland and South Asian and some other seed materials.

Methodology:

The experiment was conducted at the Phytotron (glass house) and Green House (NG 02) of the International Rice Research Institute (IRRI), Philippines during December 2013 to January 2014. Total two hundred thirty one (231) rice genotypes were screened for salt tolerance at seedling stage in hydroponics system using IRRI standard protocol (Gregorio,1997). In this experiment, FL478 and NSIC Rc222 were used as salt tolerant and sensitive checks, respectively. The screening was done under controlled environmental conditions having 29⁰C/21⁰C day/night temperature, 70% relative humidity and natural daylight inside the Phytotron. But later on, the whole population was transferred to the green house (NG 02) due to shut down of Phytotron for annual management. Firstly, the seeds were disinfected with NaOCl solution (200 ml NaCl in 1 liter water) and incubated for 48 hours to enhance germination. Eleven trays were filled up with tap water and styrofoam seedling floats were placed on the tray. Two pre-germinated seeds were sown in each hole of Styrofoam seedling float and the seedling floats were then covered with a lid for 3 to 4 days to promote germination in the dark. The tap water was replaced with Peter nutrient solution after six days of seeding and was salinized by adding crude salt to obtain EC of 12 dS/m. The volume of the Peter solution was adjusted to the level of touching the seedling float at two days interval. The pH was adjusted to 5 as well as EC was also adjusted with 12 dS/m synchronizing with the Peter solution. Tap water was added to the solution when EC was higher than 12 dS/m and NaCl was added when EC was lower than 12 dS/m. In the similar way, HNO3 was added to the solution when its pH was more than 5.0 and NaOH was added into the solution when pH was less than 5.0. Standard Evaluation System (SES) for salinity tolerance scoring was done when salt sensitive check was almost going to die or already dead. In this experiment, salt sensitive check, NSIC Rc222 was dead after two weeks of salinization. Scoring was done according to the modified standard evaluation system used in rating of the visual symptoms of salt toxicity injury (IRRI, 1997).

Source of Information: J. Biosci. Agric. Res. 10(01): 843-847

Article Link (Online Journal): DOI: 10.18801/jbar.100116.102

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Salinity tolerance seedling stage showed wide variation in phenotypes with salinity scores ranging from 3 to 9. Among 231 DS genotypes, 1.73% populations were found tolerant, 18.18% were moderately tolerant, 37.26% populations were sensitive and 46.86% were highly sensitive to salt stress. Among the genotypes, only four genotypes from irrigated population showed tolerant to salinity at 12 dS/m. As this experiment was affected by BPH , these four genotypes may also be tolerant to both salinity and BPH. Moderately tolerant populations were found from irrigated, flood prone, aerobic germination, salinity and problem soils, anaerobic germination, rainfed low land and South Asian samples. All other populations including heat tolerant samples were sensitive and highly sensitive at 12 dS/m salinity. Therefore, four tolerant genotypes found in this experiment could be further tested with 18 dS/m to determine their supremacy to salt tolerance at the seedling and reproductive stages of rice. In addition, QTL analysis should be performed to determine the effects salinity of each genomic region of the traits.

Knowledge Management: Journal