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Genetic Diversity for Reproductive Traits of Cms Restorer Lines of Rice (oryza Sativa L.)

Rubaiya Jesmin
Dept.of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur 1706, Bangladesh

M. Abdul Khaleque Mian
Dept.of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur 1706, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted to study genetic divergence in 29 CMS restorer lines of rice. The study was carried out at the experimental farm, Dept. of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during January, 2014 to June, 2014. Genetic divergence was estimated using D2 and principal component analysis. PCA showed 71.2 % variation against first Eigen values. From the scattered diagram it was revealed that the genotypes Acc-7386/ SHAKTI-1R, Acc-7394/ ACI-1R, Acc-7407/ CHINA-2R, Acc-7404/ HB-8R and Acc-7409/ 1R-509R were distantly located. Nonhierarchical clustering using co-variance matrix grouped 29 CMS restorer lines of rice into five different clusters. PCO showed that the highest inter genotype distance (2.422) was observed between the genotypes Acc-7386/ SHAKTI-1R and Acc-7394/ ACI-1R. The lowest distance (0.458) was observed between the genotypes Acc-7396/ LP-106R and Acc-7397/ LP-108R. The genotypes of cluster II exhibited the highest mean value for 11 out of 21 characters studied followed by cluster V exhibited the highest mean value for 5 out of 21 characters studied. The genotypes of cluster I exhibited the lowest mean value for 8 out of 21 characters studied followed by cluster IV exhibited the lowest mean value for 6 out of 21 characters studied. There were marked variations in intra-cluster distances, which ranged from 0.000 to 1.066. Considering magnitude of genetic distance, contribution of different characters towards the total divergence, magnitude of cluster means for different characters and performance of the genotypes Acc-7386/ SHAKTI-1R, Acc-7394/ ACI-1R, Acc-7407/ CHINA-2R, Acc-7404/ HB-8R and Acc-7409/ 1R-509R might be selected for further hybrid breeding program.

Keywords: Eigen value, Cluster means, Divergence, Non-hierarchical clustering, D2 analysis

Location: Experimental field of the Dept. of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur-1706

Start Date: 00-01-2014

End Date: 00-06-2014

Program Area: Variety and Species

Commodity: Rice

Objectives:

The present investigation was undertaken with the following objectives, to study the genetic diversity of reproductive traits among the CMS restorer lines and to identify the superior R line by comparing the reproductive traits.

Methodology:

Experimental site and design: The experiment was conducted at the experimental field of the Dept. of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur-1706, during January, 2014 to June, 2014. The experimental site is located at the center of Madhupur Tract (24°09' N latitude and 90°26' E longitude) having an elevation of 8.4m from sea level. The soil type of experimental field belongs to the shallow Red Brown Terrace type under Salna Series of Madhupur Tract (Brammer, 1971). The field is under Agro Ecological Zone (AEZ) 28 which is characterized by silly clay with pH value of 6.5. The climate of the experimental site is subtropical characterized by heavy rainfall during May to September and scanty during the rest of the year. The experiment was laid out in a Randomized Complete Block Design (RCBD) with three replications. The whole experimental area was divided into three blocks, representing three replications. Twenty nine treatments were distributed in the experimental unit of each replication through randomization by using the IRRISTAT program. Genotypes: The experiment was conducted using 29 CMS restorer lines. The seeds of all varieties were collected from genetic resources center (GRC), Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU). Collection of data: Data on phenology such as days to 10% flowering (DFF), days to 50% flowering (DF), days to maturity (DM), plant height (PH), effective tillers per plant (ETP), total tillers per plant (TTP), Panicle exertion rate (%) (PER) were recorded in time. At heading stage anther length (AL), anther breadth (AB), filament length (FL), Pollen sterility (%) (PS), Pollen fertility (%) (PF) were recorded. After maturity the selected ten hills of each genotype of each plot were harvested to record yield and yield component like Panicle length (PL), number of primary branches per panicle (NPBP), number of secondary branches per panicle (NSBP), filled grains per panicle (FGP), unfilled grains per panicle (UGP), Grain length-breadth ratio, 1000 grain weight (GW) and grain yield per plant (GYP). Statistical analysis: Genetic diversity was analyzed using GENSTAT 5.13 software program (copyright 1987, Lawes agricultural Trust, Rothamasted Experimental Station, UK). Genetic diversity analysis involves several steps, i.e., estimation of distance between the varieties clustering and analysis of inter-cluster distances. Therefore, more than one multivariate technique are required to represent the results more clearly and it is obvious from the results of many researches.

Source of Information: J. Biosci. Agric. Res. 09(01): 759-767

Article Link (Online Journal): DOI: 10.18801/jbar.090116.91

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Considering genetic diversity for reproductive traits considerable variability was observed among the 29 CMS restorer lines of rice. As per cluster analysis based on 21 characters the 29 CMS restorer lines of rice were grouped into5 clusters. The genotypes of cluster II exhibited the highest mean value for 11 out of 21 characters studied. The genotypes of cluster I exhibited the lowest mean value for 8 out of 21 characters studied. The scattered diagram for the rice genotype revealed that the genotype number Acc-7386/ SHAKTI-1R, Acc-7394/ ACI-1R, Acc-7407/ CHINA-2R, Acc-7404/ HB-8R and Acc-7409/ 1R-509R were distantly located which suggested that the genotype were more diverged from rest of the genotypes. The highest intra-cluster distances was computed for cluster III (1.066) composed of 12 genotypes. However the lowest value (0.000) of intra-cluster distance in cluster II indicated only one genotype (Acc-7386/ SHAKTI-1R) constituted this cluster might have diverged characters, which contributed to the formation of this cluster. Genotypically distant parents are usually able to produce higher heterosis. Considering magnitude of genetic distance, contribution of different characters towards the total divergence, magnitude of cluster means for different characters and performance of the genotypes Acc-7386/ SHAKTI-1R, Acc-7394/ ACI-1R, Acc-7407/ CHINA-2R, Acc-7404/ HB-8R and Acc-7409/ 1R-509R may be considered to perform better if used in hybrid breeding program. For getting precise idea about diversity molecular approach is recommended.

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