M. Oahiduzzaman
Dept.of Agricultural Chemistry, Sher-e-Bangla Agricultural University, Dhaka-1207,
S.C. Shovon
Dept.of Agricultural Botany,Sher-e-Bangla Agricultural University, Dhaka-1207
A. Mahjuba
Dept.of Agricultural Botany,Sher-e-Bangla Agricultural University, Dhaka-1207
H. Mehraj
The United Graduate School of Agricultural Science, Ehime University, Ehime, Japan
A. F. M.Jamal Uddin
Dept.of Horticulture, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh
BRRI dhan 33, Zinc, Yield, Nutrient content
Farm of Sher-e-Bangla Agricultural University, Dhaka, Bangladesh
Crop-Soil-Water Management
Fertilizer, Rice
The experiment was conducted at the Farm of Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during the period from June to November 2012 to study the effect of zinc on growth, yield and nutrient content of transplanted (T.) aman rice BRRI dhan33. The experiment consisted four levels of zinc viz. Z0: 0 kg Zn ha-1 (Control), Z1: 2.0 kg Zn ha-1, Z2: 3.0 kg Zn ha-1, Z3: 4.0 kg Zn ha-1 followed by Randomized Complete Block Design with three replications, where the experimental area was divided into three blocks representing the replications to reduce soil heterogenetic effects. The unit plot size was 3.0 m × 2.0 m and was separated from each other by 0.5 m ails. The distance maintained between two blocks and two plots were 1.0 m and 0.5 m respectively. Cowdung (5 tha-1), urea (150 kgha-1), TSP (150 kgha-1), MP (120 kgha-1), Gypsum (100 kgha-1), Borax (10 kgha-1) as recommended by BRRI, 2011 (Adunik Dhaner Chash). Entire amount of the all fertilizers and 1/3 of the urea were applied as a basal dose. Rest amount of the urea were applied in two installment, 1st installment (tillering stage)and 2nd installment (panicle initiation stages). Different doses of zinc (zinc sulphate form) were applied during final land preparation.Thirty days old seedlings of BRRI dhan33 were carefully uprooted and transplanted on 03 July, 2012 in well puddled plot. Three seedlings hill-1 were used following a spacing of 20 cm × 20 cm. After one week of transplanting all plots were checked for any missing hill, which was filled up with extra seedlings whenever required. Leaf roller (Chaphalocrosis medinalis) was observed in the field and used Malathion @ 1.12 L ha-1 to control. The crop was harvested at full maturity when 80-90% of the grains were turned into straw colored. The crop was cut at the ground level and plot wise crop was bundled separately and brought to the threshing floor. Data were collected on plant height, effective tillers hill-1, non-effective tillers hill-1, total tillers hill-1, filled grains panicle-1, unfilled grains panicle-1, total grains panicle-1, weight of 1000 seeds, grain yield, straw yield, biological yield, harvest index also content of N, P, K, S, Zn on grain samples. Data were recorded as the average of 10 plants selected at random from the inner rows of each plot. Grains and straw obtained from each unit plot were sun-dried and weighed carefully. The dry weight of grains and straw of central 1 m2 area and five sample plants were added to the respective unit plot yield to record the final grain yield plot-1 and finally converted to ton hectare-1 (tha-1). The summation of grain and straw yield were considered as biological yield. Harvest index was calculated from the grain and straw yield of rice for each plot and expressed in percentage. Chemical analysis of grain samples Determination of N:0.2 g oven dry ground sample were taken in a micro Kjeldahl flask. 1.1 g catalyst mixture (K2SO4: CuSO4.5H2O: Se in the ratio of 100: 10: 1), and 5 ml conc. H2SO4 were added. The flasks were heating at 1200C and added 2.5 ml 30% H2O2 then heated was continued at 1800C until the digests became clear and colorless. After cooling, the content was taken into a 100 ml volumetric flask and the volume was made up to the mark with de-ionized water. A reagent blank was prepared in a similar manner. Nitrogen in the digest was estimated by distilling the digest with 10 N NaOH followed by titration of the distillate trapped in H3BO3 with indicator solution and titrated with 0.01N H2SO4. Digestion of grain samples with nitric-perchloric acid for P, K, S and Zn determination: A sub sample weighing 0.5 g was transferred into a dry, clean 100 ml digestion vessel. 10ml of di-acid (HNO3: HClO4 in the ratio 2:1) mixture was added to the flask. After leaving for a while, the flasks were heated at a temperature slowly raised to 2000C. Heating were stopped when the dense white fumes of HClO4 occurred. The content of the flask were boiled until they were became clean and colorless. After cooling, the content was taken into a 100 ml volumetric flask and the volume was made up to the mark with de-ionized water. P, K, S and Zn were determined from this digest Determination of P: Phosphorus in the digest was determined by using 1 ml for grain sample from 100 ml extract was then determined by developing blue color with reduction of phosphomolybdate complex using ascorbic acid and the color intensity were measured colorimetrically at 660 nm wavelength and readings were calibrated with the standard P curve. Determination of K: Five ml of digest sample for the grain were taken and diluted 50 ml volume to make desired concentration so that the emission of K of sample were measured within the range of standard solutions. The K was determined by flame photometer. Determination of S: Sulphur content was determined from the digest of the grain samples (with BaCl2 solution as described by Page et al. 1982. The digested S was determined by developing turbidity by adding BaCl2 seed solution. The intensity of turbidity was measured by spectrophotometer at 420 nm wavelengths (Hunter, 1984). Determination of Zinc: Zinc content was determined from the digest of the grain samples (with BaCl2 solution as described by Page et al. 1982. The digested Zn was determined by developing turbidity by adding BaCl2 seed solution. The intensity of turbidity was measured by spectrophotometer at 420 nm wavelengths (Hunter, 1984). Collected data were statistically analyzed using MSTAT-C program. The significance of the differences among the treatment means were estimated by the Duncan’s Multiple Range Test (DMRT) at 1% level of probability (Gomez and Gomez, 1984).
J. Biosci. Agric. Res. 09(02): 820-826
Journal