Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Propagation of Two Strawberry (fragaria Ananassa Duch.) Cultivars of Bangladesh

Tapati Roy
Agrotechnology Discipline, Khulna University, Khulna-9208, Bangladesh

S.M. Abdullah Al Mamun
Agrotechnology Discipline, Khulna University, Khulna-9208, Bangladesh

Md. Monirul Islam
Agrotechnology Discipline, Khulna University, Khulna-9208, Bangladesh

Abstract/ Executive Summary:

An experiment on in vitro propagation of strawberry was carried out at Plant Breeding and Biotechnology Laboratory of Agrotechnology Discipline, Khulna University, Khulna, Bangladesh during the period of February to November, 2015. In the experiment, runners of two strawberry cultivars viz. BARI Strawberry^-1 and Modern Strawberry-5 (Festival) were cultured on MS media supplemented with different concentrations of BAP (1.0, 1.5 and 2.0 mgL^-1) and Kin (0.1, 0.5 and 1.0 mgL^-1) for multiple shoot regeneration. The experiment was laid out in Completely Randomized Design (CRD) with three replications. The highest number of shoots (10.41) was obtained from Modern Strawberry-5 and maximum shoot length (13.62 mm) obtained from BARI Strawberry-1. Maximum number of shoots (13.33) and shoot length (24.29 mm) were recorded from MS media containing 1.0 mgL^-1 BAP and 1.0 mgL^-1 Kin. Then all the regenerated plantlets were cultured on MS media containing 2.0 mgL^-1IAA for root initiation. Greater reduction in vigor was observed in Modern Strawberry-5 (Festival) when they were transferred to root induction media. Ex vitro survivability of the complete plantlets varied from 36.67 to 6.67%. Maximum survivability of both varieties 36.67% (BARI Strawberry^-1) and 26.67% (Festival) were recorded from MS media supplemented with 1.0 mgL^-1 BAP and 1.0 mgL^-1 Kin. For in vitro micropropagation, BARI Strawberry-1 may be better and MS media supplemented with 1.0 mgL^-1 BAP and 1.0 mgL^-1 Kin may be better.

Keywords: Strawberry, Benzyl adinopurine, Kinetin, Micropropagation, In vitro Propagation

Location: Plant Breeding and Biotechnology Laboratory, Agrotechnology Discipline, Khulna University, Khulna, Bangladesh

Start Date: 00-02-2015

End Date: 00-11-2015

Program Area: Variety and Species

Commodity: Strawberry

Objectives:

To optimizing the concentration of growth regulators for in vitro clonal propagation of two strawberry cultivars of Bangladesh.

Methodology:

The experiment was carried out at Plant Breeding and Biotechnology Laboratory, Agrotechnology Discipline, Khulna University, Khulna, Bangladesh from February to November, 2015. For the research, two cultivars of strawberry viz. BARI (Bangladesh Agricultural Research Institute) Strawberry-1 and Modern Strawberry-5 (Festival) were selected and MS media supplemented with nine treatments consisting of different concentrations and combinations of BAP and Kin were as follows: 1) 1.0 mgL^-1 BAP and 0.1 mgL^-1 Kin, 2) 1.5 mgL^-1 BAP and 0.1 mgL^-1 Kin, 3) 2.0 mgL^-1 BAP and 0.1 mgL^-1 Kin, 4) 1.0 mgL^-1 BAP and 0.5 mgL^-1 Kin, 5) 1.5 mgL^-1 BAP and 0.5 mgL^-1Kin, 6) 2.0 mgL^-1 BAP and 0.5 mgL^-1Kin, 7) 1.0 mgL^-1 BAP and 1.0 mgL^-1 Kin, 8) 1.5 mgL^-1 BAP and 1.0 mgL^-1 Kin, 9) 2.0 mgL^-1 BAP and 1.0 mgL^-1 Kin. The experiment was laid out in Completely Randomozed Design (CRD) with three replications. There were total 18 treatment combinations in each replications. BARI Strawberry-1 was collected from Ankur nursery, Jessore and Modern Strawberry-5 (Festival) was collected from Modern Horticultural Center, Nator. These collected plant materials (Germplasm) were grown and conserved in the field Laboratory (BARI Strawberry-1) of Agrotechnology Discipline and in the field of Plant Breeding and Biotechnology Laboratory (Modern Strawberry-5 (Festival)) of Agrotechnology Discipline, Khulna University, Khulna. Shoot (runner) tips of the both varieties were used as explant. Full strength MS (Murashige and Skoog, 1962) media supplimented with BAP (1.0, 1.5 and 2.0 mgL^-1) and Kin ( 0.1, 0.5 and 1.0 mgL^-1) were used for in vitro shoot multiplication. MS media supplemented with 2.0 mgL^-1 IAA was used for rooting of the in vitro grown shoots. Fresh runners from healthy field grown strawberry plants were collected from the fields of Plant Breeding and Biotechnology Laboratory, Agrotechnology Discipline, Khulna University, Khulna. The runner tips (about 2.0 cm) were separated by scissors and washed for five times throughly under running tap water to remove the dusts. The tips were then treated with with few drops of Tween-20 for 10 minutes followed by shaking with 0.05 gL^-1 NADCC at 37 °C for three hours in shaking incubator at 70 pv (shake/minute). The runner tips were then transferred to laminar airflow cabinet and washed with autoclave distilled water for 3 times. They were then sterilized with 70 % ethanol for 30 seconds followed by 0.2 % HgCl₂ for 8 minutes. Thereaf- ter, they were washed with autoclave distilled water for 3 times to wash out the traces of HgCl₂. About 1.5 to 2.0 cm from shoot tips after surface sterilization, were cultured on MS medium with the help of sterilized forceps into the cultured bootle each containing 30 ml semi-solid medium under laminar airflow hood. After the inoculation, the cultures were kept under fluorescent light in growth chamber with controlled temperature (25±1°C) under 16 hours photoperiod with light intensity of 2000-3000 lux and relative humidity 80±2 % for three weeks to allow multi shoot regenaration. All cultures were checked daily and any culture showing symptoms of contamination were discarded immediately. In the present experiment, data were collected for evaluation on the following parameters: 1) Number of shoot culture-1, 2) Length of shoot (mm) and 3) Survivability (%). Here, two sub-cultures were done, each at 30 days interval in agarifird MS medium supplemented with same concentrations of BAP and Kin. Data on shoot number and shoot length per culture were encored. After 90 days of first culture, the regenerated multiple shoots were cultured in MS medium containing IAA at the rate of 2.0 mgL^-1 for root initiation. The regenerated plants were then transferred to small pots containing well prepared soil. They were kept for observation. Data on survivability was then recorded by using the following formula: Survivability (%) = No. of plants survived after 3 weeks/Total no. plants transferred to pots x 100. The obtained data of the experiment were analyzed for varience (ANOVA) with the help of computer package program MSTAT-C in computer. Differences among the treatment means were compared by New Duncans Multiple Range Test (DMRT) (Gomez and Gomez, 1984).

Source of Information: Archives of Agriculture and Environmental Science 3(4): 382-387 (2018)

Article Link (Online Journal): DOI: 10.26832/24566632.2018.030409

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

For in vitro clonal propagation of strawberry, the maximum number shoots at 90 DAC was recorded 10.41 from Modern Straw- berry-5 (Festival) and 10.33 from BARI Strawberry-1. The highest number of shoot regeneration was obtained from MS medium supplemented with growth regulators 1.0 mgL^-1 BAP + 1.0 mgL^-1 Kin 90 DAC (13.33). The survivability of the regenerated plants varied from 10.00% to 36.67% in case of BARI Strawberry-1 and 6.67% to 26.67% for Modern Strawberry-5. In in vitro clonal propagation of strawberry, MS medium supplemented with growth regulators combination of 1.0 mgL^-1 BAP and 1.0 mgL^-1 Kin may be the best for shoot multiplication and between the two varieties, BARI Strawberry-1 may perform better in respect of

Knowledge Management: Journal