Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Isolation and Identification of Common Fungal Spp. from Commercial Broiler Feeds Available in Market of Sylhet District, Bangladesh

MT Islam
Department of Microbiology and Immunology, Sylhet Agricultural University, Sylhet-3100, Bangladesh

MM Rahman
Department of Medicine, Faculty of Veterinary and Animal Science, Sylhet Agricultural University, Sylhet-3100, Bangladesh

M Purkayastha
Department of Microbiology and Immunology, Sylhet Agricultural University, Sylhet-3100, Bangladesh

ATMM Elahi
Department of Microbiology and Immunology, Sylhet Agricultural University, Sylhet-3100, Bangladesh

MK Hossain
Department of Microbiology and Immunology, Sylhet Agricultural University, Sylhet-3100, Bangladesh

Abstract/ Executive Summary:

The present study was designed to investigate the mycological contamination of commercial broiler feeds used in poultry establishments in sylhet, Bangladesh. The feed samples of commercial broiler feed (Starter, Grower and Finisher) were collected from the different areas of Sylhet district. A total of 189 commercial broiler feed samples where 63 Starter, 63 Grower and 63 Finisher were collected from the different areas of local market in Sylhet. The selected areas were Kadamtali, Shibjong, Khadim, Kamal Bazar, Dakshin Surma, Fenchugonj. From the feed samples analyzed for the presence of fungal agents, 144 (76.2%) were found positive for one or more fungal species. Fungal isolates were found among 36 (57%) of the 63 Starter feed samples, 45 (71.4%) of the 63 Grower feed samples and 63 (100%) of the 63 Finisher feed samples. The fungal agents isolated from Broiler Starter Feeds, Aspergillus spp. 51 (70.8%) has the highest frequency of occurrence, followed by Fuserium spp. 12 (16.7%) and least is Rhizopus sp. 9(12.5%). Similarly, in case of Broiler Grower Feeds, Aspergillus spp. 66 (68.8%) has the highest frequency of occurrence, followed by Fuserium spp. 18 (18.7%) and least is Rhizopus sp. 12(12.5%). In case of Broiler Finisher Feeds, Aspergillus spp. 90 (69.8%) has the highest rate of occurrence followed by Fuserium spp. 24 (18.6%) and least is Rhizopus sp. 15 (11.6%) respectively.

Keywords: Commercial Broiler feed, Isolation, Aspergillus spp., Fuserium spp., Rhizopus spp.

Location: laboratory of Microbiology and Immunology, Sylhet Agricultural University, Sylhet

Start Date: 00-07-2013

End Date: 00-12-2013

Program Area: Animal Health and Management

Commodity: Broiler, Feed

Objectives:

The present study is proposed with the objectives of isolation of fungal species from Commercial broiler feed samples and identification of the common fungal species from isolates.

Methodology:

The research work was conducted in the laboratory of Microbiology and Immunology, Sylhet Agricultural University, Sylhet from July to December 2013. The feed samples of commercial broiler feed (Starter, Grower and Finisher) were collected from Kadamtali, Shibjong, Khadim, Kamal Bazar, Dakshin Surma and Fenchugonj, Sylhet. The total study was performed in three (3) steps. The first step included feed sample collection, transportation and preservation. The second step of the experiment was isolation of fungal genera from commercial broiler feed sample and the third step was identification of common fungal spp. from pure culture. The Commercial Broiler feed samples were collected from different farms and dealers for the experimental study. A total number of 189 field samples were aseptically collected into sterile plastic bag from different spots and carried to the laboratory for the isolation and identification of common fungal Species. Firstly,Ten-fold serial dilution of 1g of feed with distilled water then 0.1ml of the dilution was cultured by spread plate technique into Potato dextrose agar (PDA) supplemented with chloramphenicol at 40 µg/ml and Gentamycin at 500 µg/ml and incubated for 5 to 14 days at room temperature. Pure culture of the different colonies (based on morphology) was obtained by sub-culture of the isolates on potato dextrose agar plates and sabouraud’s dextrose agar plates. The fungal isolates were identified to the genus/species level based on macroscopic and microscopic characteristics of the isolates obtained from pure cultures. Nutrient broth was prepared by dissolving 13 gms of dehydrated nutrient broth into 1000 ml of distilled water and was sterilized by autoclaving at 121°C under 15 pounds pressure per square inch for 15 minutes (1kg/cm²). Then the broth was dispensed into tubes (10 ml/tube) and stored at 4°C in the refrigerator until used. For preparation of phosphate buffer saline, 8 grams of sodium chloride (NaCl), 2.89 grams of disodium hydrogen phosphate (Na₂HPO₄.12H₂O), 0.2 grams of potassium chloride (KCl) and 0.2 grams of potassium hydrogen phosphate (KH₂PO₄) were suspended in 1000 ml of distilled. The solution was heated to dissolve completely. The solution was then sterilized by autoclaving at 12l°C maintaining a pressure of l5 pounds per sq. inch for 15 minutes (1kg/cm²) and stored at 4-8°C until used. The pH of the solution was measured by a pH meter and maintained at 7.0-7.2. A total of 64 gms of media were properly mixed with 1000 ml distilled water and boiled to dissolve the medium completely. It was sterilized using autoclaving at 15 lbs pressure (121°C) for 15 minutes (lkg/cm²) and mixed well before dispensing to start aseptically and with proper care so that any contamination be avoided. A total of 65 gms of media were properly mixed with 1000 ml distilled water and boiled to dissolve the medium completely. It was sterilized using autoclaving at 15 lbs pressure (121°C) for 15 minutes (lkg/cm²) and mixed well before dispensing to start aseptically and with proper care so that any contamination be avoided. An inoculum is prepared from the sample and streaked on the SDA media and incubated at room temperature. The different plate media were examined for the fungal growth every day until growth is found after 3 to 4 days of plating. Pure culture was prepared from this initial culture ^[18]. In order to make a pure culture, spores from initial culture was transferred to media containing petridishes by sterilized inoculating loop for avoiding any contamination with other fungus and incubate for 3-4 days at room temperature until the fungal growth is found. From pure culture, fungal colony was taken with the help of an inoculating needle on a fresh glass slide containing two drops of lactophenol cotton blue. The fungal colony was covered with a cover slip and the slides were examined under the microscope. The fungus was identified on the basis of its cultural and morphological characteristics.

Source of Information: International Journal of Natural Sciences (2014), 4(2) 38-41

Article Link (Online Journal): DOI: https://doi.org/10.3329/ijns.v4i2.28603

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From the study it may be concluded that, in starter feed, the company used antifungal inclusions and less used such things in grower and finisher feeds. As fungal diseases are sensitive for commercial broilers that’s why different feed company use high level of fungistat during feed processing. But, this practice is harmful for public health. The use of strong antifungals in every level of feeds (Starter, Grower and Finisher) may reduces the fungal contamination of feeds when storage at dealer and farm levels; On the other hand, the feed mill manufacturing process should be maintained properly and post processing contamination should be strictly avoided. Therefore, the occurrence of fungi in commercial broiler feeds may be due to pre and post processing contamination of feed ingredients, bad manufacturing process, contamination of the feed by handlers in the farm, bad feed storage facilities in the farm among others. Due to this fact, regular microbiological and mycotoxicological analysis should be performed for maintaining quality and safety of poultry feed.

Knowledge Management: Journal