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Serum Testosterone Concentration in Surgically Castrated Black Bengal Goats

M Hasan
Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh -2202, Bangladesh

M A H Miah
Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh -2202, Bangladesh

T A Rosy
Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh -2202, Bangladesh

P K Jha
Scientist (S -1),
Nepal Agricultural Research Council (NARC), National Animal Science Research Institute (NASRI), Animal Breeding Division, Khumaltar, Lalitpur, Nepal

N S Juyena
Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh -2202, Bangladesh

Abstract/ Executive Summary:

The experiment was carried out to detect the blood serum testosterone concentration after orchiectomy in Black Bengal bucks (Capra hircus). Twelve apparently healthy bucks of 8 -10 months old were randomly divided into two groups. Group A were castrated surgically through open uncover method and group B remained intact. Local analgesia (2% lidocaine hydrochloride) @ 0.5 mL per spermatic cord and 0.5 mL at the tip of each scrotum were applied initially in all bucks. After aseptic preparation, incision was given at the tip of the scrotum and tunica vaginalis was incised to exteriorize the testis. Anchoring was done at the spermatic cord followed by orchiectomy. The scrotal raphe was incised to reach the other testis and the same procedure was followed. Blood samples were collected from jugular vein before orchiectomy and on day 30. In the castrated bucks, serum testosterone concentration was significantly (P<0.01) decreased from day 0 (6.1 ± 0.2 ng/ mL) to day 30 (0.6 ± 0.0 ng/ mL), which confirmed the efficacy of castration.

Keywords: Black Bengal Bucks, Castrtion, Testosterone

Location: Research Animal Farm Laboratory, Department of Surgery and Obstetrics, BAU, Mymensingh

Start Date: 01-07-2016

End Date: 30-11-2016

Program Area: Animal Health and Management

Commodity: Goat

Objectives:

To determined blood serum testosterone concentrations after castration of Bucks

Methodology:

Ethical statement: Orchiectomy was done aseptically. Local analgesia was done prior to orchiectomy. Painkiller was administered as post -castration pain management. Experimental site: The research was carried out in the Research Animal Farm Laboratory, Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh, from July 2016 to November 2016. Study population:Twelve male Black Bengal goats (bucks) 8 – 10 months old were purchased from local market. The bucks weighed 12 – 15 kg and no visible abnormalities or disease were detected. The bucks were kept in quarantine for 14 days and were supplied with grass, concentrate feed and water. The goat-shed was regularly washed and disinfected with 0.1% potassium permanganate solution. Ivermectin 0.2 mg/ kg was injected subcutaneously to each buck. Tetanus toxoid vaccine and PPR vaccine were administered to each buck before the experiment. Experimental protocol: The bucks were acclimatized and divided into two groups of 6, Group A were castrated, group B were control. ELISA was used to determine the blood serum testosterone concentrations. Observation of clinical parameters: Rectal temperature, heart rate and respiration rate were measured before orchiectomy (day 0) on day 1 to 7, on day 15 and day 30 post -castration. Orchiectomy procedure: Open uncover method was applied. Initially local analgesia was done using 2% lidocaine hydrochloride @ 0.5 mL per spermatic cord and @ 0.5 mL at the tip of the scrotum. The operation site was shaved, scrubbed with soapy water and painted with tincture of iodine. After aseptic preparation, incision was made at the tip of scrotum and tunica vaginalis was incised. The testis was press ed firmly and spermatic cord was exteriorized. Spermatic cord was anchored using chromic catgut no. 1 followed by orchiectomy distal to epididymis. The scrotal raphe was incised to reach the other testis and same procedure was followed. Antibiotics (Procai ne penicillin 30 lac IU and Benzyl penicillin 10 lac IU; Pronapen ®) antihistaminic (Pheniramine maleate @ 1.0 mg/ Kg body weight; Asta -vet ®) and anti -inflammatory drugs (Ketoprofen dosed @ 3.0 mg/ Kg body weight; Ketovet ®) were administered. Hormonal assay of testosterone using ELISA technique: Blood serum collection - Briefly, 2 mL blood samples were collected from left jugular vein following aseptic measures, before orchiectomy and on day 30 using vacuum blood collection tube. Blood samples were centrifuged @ 3000 rpm for 30 minutes. Afterwards, serum were collected in Eppendorf tubes and kept at -20°C. ELISA procedure - Serum samples were equilibrated at room temperature for about 30 minutes. The testosterone ELISA kit was purchased from Nova TecImmunodiagnostica GmbH (Germany). For testosterone assay, biotinylated antibody, enzyme -antigen conjugate and a native antigen containing serum were used as the essential reagents. The biotinylated antibody is the anti -testosterone biotinylated purified rabbit IgG conjugate. Horseradish peroxidase is the enzyme -antigen conjugate. Standard instructions were followed. Testosterone concentration was measured in ng/ mL. Calculations were done using the formula y = a + b × x from a standard curve with seven standards supplied with the testosterone ELISA kit box. At 450 nm, the optical density (OD) values of the samples and stand ards were determined using the spectrophotometer (ELISA reader) Ultra Microplate reader. Statistical analysis: One way analysis of variance (ANOVA) was applied, using the software IBM SPSS Statistics, Version 20. The level of significance was considered at P<0.05.

Source of Information: The Bangladesh Veterinarian (2016) 33(2) : 71 – 77

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Testosterone concentration in blood serum could be used as a major determinant in evaluating the efficacy of castration in Black Bengal bucks.

Knowledge Management: Journal