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Comparative Study on Farm Materials and Quality Parameters of Pangus (pangasianodon Hypophthalamus) of Different Farms in Trishal Area

M. I. Hossain
Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

F. H. Shikha*
Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

T. Chakrabarty
Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The present study was conducted to carry out a comparative study on farm materials (water, soil, feed) and the quality parameters of pangus (Pangasianodon hypophthalamus) of five different farms in Trishal area under Mymensingh district. The study was carried out during May-June, 2016. Most of the pangus farmers used homemade or farm made supplementary feeds for pangus culture The percent moisture, protein, lipid and ash content in fresh fish ranged from 77.05 to 78.79, 14.21 to14.84, 3.55 to 4.49 and 2.03 to 2.67 whereas after 24 hrs of death of fishes, these percent values changed as 77.05 to 78.79, 14.21 to14.84, 3.55 to 4.49 and 2.03 to 2.67. Slight changes observed in different quality parameters in the fish samples 24 hrs after death as dead fishes were preserved at chilling temperature in a homestead refrigerator till analysis. Therefore, the study could be concluded that- though the values for different parameters of farm materials (water, soil, feed) and quality parameters of fish samples differed but was mostly not significant. The differences observed in the parameters might be due to the variations in management techniques of each farm.

Keywords: Comparative study, Farm material, Pangus, Quality parameter

Location: Trishal area under Mymensingh district

Start Date: 00-05-2016

End Date: 00-06-2016

Program Area: Animal Health and Management

Commodity: Pangus

Objectives:

The present experiment was designed to carry out a comparative study on farm materials and fish samples of randomly selected five farms of Trishal area under Mymesingh district which might produce necessary information for pangus aquaculture.

Methodology:

Period of the study The study was carried out during May-June, 2016. Within this period the sampling of pangus was done and the biochemical and microbiological analysis of waters, soil (sediment), feed and fish samples were done in the laboratories of Fish Processing and Fish Microbiology, Department of Fisheries Technology and Central Laboratory, Bangladesh Agricultural University. Sampling of farm The study was conducted on a total of 5 pangus aquaculture farms of Mathbari, Dhanikhola and Sakhua village in Trishal upazila under Mymensingh district. The sampling was done in random manners. Collection of water samples Water samples were collected from three different places of each selective pond (where the depth of water was of 20 cm from surface). Water samples were collected in clean plastic bottles marked with respective farm numbers and transferred to Professor Muhammed Hussain Central Laboratory, Bangladesh Agricultural University, Mymensingh to determine the PH and TDS (Total Dissolved Solids) in water. Collection of soil samples Small plastic bags marked with the farm numbers were used for soil samples collection. Bottom soil samples from three different places of each selected ponds were collected, brought to Fish Processing Laboratory, Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh and kept for drying in the air. After completion of drying, the soil samples were ground and sieved by fine mesh sieve for analysis. pH and TDS (Total Dissolved Solids) of soil samples were analyzed in Professor Muhammed Hussain Central Laboratory, BAU, Mymensingh. Collection of feed samples Feed samples from five different farms were collected in plastic bags and marked. The samples were then brought to Fish Processing Laboratory, Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh for proximate composition analysis. Before proximate composition analysis the feed samples were ground and sieved by fine mesh. AOAC (1990) method was followed for proximate composition analysis. Collection of pangus fish s amples and biochemical analysis of quality parameters of sample fishes Ten live freshwater pangus (Pangasianodon hypophthalamus) were collected from five farms of Trishal, Mymensingh during harvesting and brought to the laboratory of Fisheries Technology and kept in different buckets containing tap water. At first five live fishes collected from different farms were sacrificed for biochemical analysis. The other five fishes were kept in separate buckets until they die and pass in rigor stage. After 24 hrs of death of the fishes another set of biochemical analysis was done. The samples collected from dorsal, ventral and caudal part of fish body. Proximate composition AOAC (1990) method was followed for bio-chemical analysis (percent moisture, ash, protein, lipid of feed samples and fish samples). Homogeneity of the samples was maintained by using a blender. For each analysis of proximate triplicate samples were used. TVB-N (Total volatile base nitrogen) Total Volatile Base Nitrogen (TVB-N) of fish samples was determined according to the methods given in AOAC (1990) with certain modification. Muscle pH For the determination of fish muscle pH 10g of sample was taken and homogenized with 50ml of distilled water in a blender. After adjusting the temperature, the electrode of the pH meter was put on the tube and the pH was measured by the pH meter (Lutron pH - 222) in duplicate. pH of water and soil pH of collected water and soil samples from five selected farms of Trishal area was measured in Professor Muhammed Hussain Central Laboratory Bangladesh Agricultural University, Mymensingh using a pH meter (Meltler Toledo EL-20). TDS (Total dissolved solids) in water and soil TDS (total dissolved solids) of collected water and soil samples from five selected farms of Trishal area was determined in Professor Muhammed Hussain Central Laboratory, Bangladesh Agricultural University, Mymensingh using a TDS machine (Hanna Instrument Model-Hl 98194). Microbiological Analysis Water sample Appropriate dilutions of water samples (three samples from each pond) were made (10-1 to 10-6) with sterile physiological saline in deionized water. Aliquots of 0.1 ml of the serial dilutions were inoculated onto nutrient media in duplicate using the spread plate method. The samples pipetted were spread by L-shaped glass rods and put in incubator. Soil sample Five gram of wet sediment sample from each pond was weighted and dissolved in 200 ml physiological saline to make stock solution. One milliliter of the homogenate was serially diluted (10-1 to 10-6) and treated in the same way as the water samples. Microbial study of sediment samples was done in similar manner as done for water sample. Fish sample 10 gm of fish sample was taken out aseptically with sterile knife from the body of fish of each pond and then homogenized with 90ml of sterile saline solution (0.85% NaCl) in a sterile blender to obtain 1:10 dilution. One ml of diluted samples was transferred with a sterile pipette to a test tube containing 9.0ml of 0.85% physiological saline and the test tube was shaken thoroughly for mixing. Using similar process several dilutions were made up to the desired level. For microbiological analysis of fish sample also same procedure followed and calculation was done using the formula given below.

Source of Information: J. Environ. Sci. & Natural Resources, 11(1&2):109- 119, 2018 ISSN 1999-7361

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Achievement/Findings:

From the obtained results the study could be concluded that- differences observed for various parameters of farm materials (water, soil, feed) and fish samples among randomly selected five farms was mostly not significant and the little differences occurred might be due to the variation in the management techniques of each farm.

Knowledge Management: Journal