Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Micropropagation of Jasminum Grandiflorum L.

Md. S. Rahman*
BCSIR Laboratories Chittagong, Chittagong-4220, Bangladesh

N. J. Mouri
BCSIR Laboratories Chittagong, Chittagong-4220, Bangladesh

N. C. Nandi
BCSIR Laboratories Chittagong, Chittagong-4220, Bangladesh

S. Akter
BCSIR Laboratories Dhaka, Dhaka-1205, Bangladesh

Md. S. Khan
BCSIR Laboratories Dhaka, Dhaka-1205, Bangladesh

Abstract/ Executive Summary:

An efficient protocol was developed for in vitro plant regeneration of Jasminum grandiflorum L. The highest elongated shoots (60%) were achieved from axillary meristems using MS (Murashige and Skoog ) basal medium supplemented with 1 mg/l 6-benzyladenine (BAP) and 60 mg/l coconut water. After adding 1.0 mg/l BAP and 45 mg/l coconut water in the culture medium, the highest rate of shoot proliferation was exhibited after 4 weeks of culture. Rooting was found within 14-23 days after the cut end of shoots was soaked in 1.5 mg/l IBA solution for 3-7 minutes. The regenerated healthy rooted plantlets were transferred to small plastic pot containing garden soil and compost in a ratio of 2:1. Maximum (75%) in vitro rooted plants were survived in the shade house and finally survived naturally in soil condition. The successful protocol for in vitro regeneration was developed which will facilitate the conservation and propagation of the important medicinal plant.

Keywords: In vitro regeneration; Mass propagation; Jasminum grandiﬂorum, Explant

Location: BCSIR Laboratories Chittagong, Chittagong-4220, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Jesmine

Objectives:

The present study was to investigate and to develop a suitable protocol for micropropagation of J. grandiflorum. This finding may be helpful for the establishment of micro-propagation techniques to produce rapid and clean clones of J. grandiflorum. It may be of great value for the future research studies.

Methodology:

Plant material Elongated healthy shoots (8-10 cm long) of J. grandiflorum were collected from Bangladesh Council of Scientific and Industrial Research (BCSIR) Laboratories, Chittagong campus and used as sources of explants for this experiment. Shoot tips and stem nodes with a single axillary bud were used. The explants were surface sterilized with soft detergent for three times followed by washing with a few drops of Tween 20 and thoroughly washed in running tap water for 20-25 minutes. To remove surface contamination the stem segments were put in a 0.1% (m/v) aqueous mercuric chloride solution for 12 min. Thereafter, stem segments were washed 4-5 times with autoclaved distilled water to remove all traces of HgCl₂ and finally cut into smaller segments (about 1.3 cm long) each with one node. Shoot regeneration The sterilized fresh nodal explants were transferred to MS medium (Murashige and Skoog, 1962) supplemented with various concentrations of cytokinins, i.e. 6-benzylaminopurine (BAP: 0.0, 0.5, 1.0 and 1.5 mg/l), kinetin (Kn: 0.0, 0.5, 1.0 and 1.5 mg/l) and coconut water (30, 45 and 60 ml/l for shoot regeneration. The pH of the medium was adjusted to 5.8 before autoclaving. One excised stem segment was cultured in each tube (25 × 150 mm) with about 20 ml of the culture medium. The cultures were maintained at 16 hrs photoperiod at 25 ± 2°C temperature with white fluorescent lamps having a light intensity of 55 µE/m2/s. For shoot regeneration 20 replicates per treatment were used in each experiment. The percentages of shoots formation from segments were examined periodically up to 4 weeks of culture and the work was repeated three times. Subculture was also carried out in a 2-week interval. Root regeneration and acclimatization After development, elongated shoots (5-6 cm long) were excised and separated and the cut ends of separated shoot were soaked into different concentration (5, 10 and 15 mg/l) of IBA solution for 3-7 minutes. With the help of sterilized blotting paper extra IBA solution attached onto each shoot was removed and finally the treated shoots were transferred into previously autoclaved plastic pot containing garden soil and compost in a ratio of 2:1 and moistened them adequately for proper hardening. At one week interval, 2-3 ml MS solution was supplied in each pot. After 4 weeks of culture, the number of roots per shoot and the percentage of shoots forming roots were examined. Finally the well rooted plantlets were transfered into the field.

Source of Information: Bangladesh J. Sci. Ind. Res. 53(4), 277-282, 2018

Article Link (Online Journal): DOI: http://dx.doi.org/10.3329/bjsir.v53i4.39191

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In conclusion, it can be said that, successfully production of shoots and in vitro root induction of J. grandiflorum were dependent on growth regulators and the culture conditions. This study might open a new concept for mass propagation and germplasm conservation of J. grandiflorum.

Knowledge Management: Journal