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In Vitro Regeneration of Sugarcane Through Somatic Embryogenesis

R.K. Ganapati
Bangladesh Sugarcrop Research Institute, Ishwardi, Pabna-6620, Bangladesh

M.R. Alam
Bangladesh Sugarcrop Research Institute, Ishwardi, Pabna-6620, Bangladesh

M.S.A. Mamun
Bangladesh Sugarcrop Research Institute, Ishwardi, Pabna-6620, Bangladesh

R.C. Kabiraj
Bangladesh Sugarcrop Research Institute, Ishwardi, Pabna-6620, Bangladesh

D R. Rani
Department of Zoology, B M College, Barisal, Bangladesh.

Abstract/ Executive Summary:

A simple and efficient method of plant regeneration from midrib-derived callus was established in sugarcane. Explant was cultured in MS medium supplemented with 2,4-D and BA callus induction. The highest callus induction was found in MS medium supplemented with 2 mgl^-1 2,4-D and 0.2 mgl^-1 BA, where 72% of explants produced callus. Two month calli were subcultured in to same medium which has shown different types and color. The highest frequency of shoot formation was obtained in MS medium supplemented with 1.0 mgl^-1 BA and 0.5 mgl^-1 NAA. Half strength MS supplemented with 3 mgl^-1 NAA performed best for root induction. After acclimatization of in vitro grown plantlet, 85% were survived.

Keywords: Mid rib, Callus induction, Plant regeneration, Sugarcane

Location: Plant Tissu Culture laboratory, Breeding Division, Bangladesh Sugarcrop Research Institute

Start Date: 00-00-2015

End Date: 00-00-2015

Program Area: Variety and Species

Commodity: Sugarcane

Objectives:

To established a protocol for large scale production of sugarcane plantlet through somatic embryogenesis.

Methodology:

The experiment was conducted at Plant Tissue Culture laboratory, Breeding Division, Bangladesh Sugar crop Research Institute during autumn 2015. Clean and healthy tops (shoot) of 8-10 months old of five sugarcane clones Isd 20, Isd 35, I 220-92, Co 65-02 and I 214-11 were collected as plant materials. Immature leaf rolls were collected for explant preparation. Leaf roll was washed thoroughly under running tap water followed by 70% alcohol for 1 hr. The plant material was taken into laminar flow cabinet and surface sterilized with 30% Clorox for 20 minutes and rinsed 3 times with sterile double distilled water. Media were prepared and pH was adjusted to 5.75 and autoclaved at 121ºC having pressure 15 lbs for 20 min. All cultures were incubated at 25±2ºC and kept under 16 h photoperiod of fluorescent tube light. The sterile leaf rolls were sectioned crosswise followed to longitudinal to harvest midrib(explant). Then aseptically cultured on modified MS medium (Murashige and Skoog, 1962) supplemented with different concentration of BA and 2, 4- D for callus induction. After 20 days explant with immersing calli were subculture for maturation. Callus was then transferred in MS supplemented with different concentrations and combinations of cytokinin (BA) and auxin (NAA) for shoot regeneration. The in vitro grown micro-shoots were inoculated in the half strength MS media supplemented with different concentrations of auxin (IBA and NAA) for root initiation. These micropropagated plantlets were hardened and planted in poly bags and kept inside the poly house for acclimation. Completely randomized design was used in experiment and data were statistically analyzed using Statistix 10.

Source of Information: Int. J. Sustain. Crop Prod. 11 (3): August 2016

Article Link (Online Journal): http://ggfjournals.com/e-journals archive

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Callus induction was found suitable in MS medium supplemented with 0.2 mgl^-1 BA and 2 mgl^-1 2,4-D. Soot induction was suitable in 1.5 mgl^-1 BA and 0.5 mgl^-1 NAA supplemented MS medium. Half MS medium supplemented with 3.0 mgl^-1 NAA is the best for rooting.

Knowledge Management: Journal